Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production
The global shift towards renewable energy has heightened the importance of bioethanol as a sustainable alternative to fossil fuels, addressing environmental concerns and reducing greenhouse gas emissions. Sustainable chemistry offers innovative solutions for converting agricultural residues into val...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Sustainable Chemistry for the Environment |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949839225000343 |
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| author | Sudarshan Sahu Urbi Bansal Gursharan Singh Shailendra Kumar Arya |
| author_facet | Sudarshan Sahu Urbi Bansal Gursharan Singh Shailendra Kumar Arya |
| author_sort | Sudarshan Sahu |
| collection | DOAJ |
| description | The global shift towards renewable energy has heightened the importance of bioethanol as a sustainable alternative to fossil fuels, addressing environmental concerns and reducing greenhouse gas emissions. Sustainable chemistry offers innovative solutions for converting agricultural residues into valuable biofuels, yet challenges in optimizing pretreatment and enzymatic processes persist. This study addresses these gaps by systematically comparing physical, chemical, physicochemical, and enzymatic pretreatments to enhance ethanol yields from paddy straw. Methods included alkali, sonication, and alkali-assisted sonication treatments to modify substrate composition, followed by enzymatic hydrolysis using cellulase, xylanase, and mannanase. Results revealed that alkali-assisted sonication yielded the highest reducing sugar concentrations (30 ± 0.8 mg/mL) and ethanol productivity (0.41 g/L/h), with a saccharification percentage of 89 % and ethanol yield of 0.58 g/L. In contrast, xylanase exhibited a saccharification percentage of 83 % with an ethanol productivity of 0.28 g/L/h, while cellulase achieved 85 % saccharification and 0.35 g/L/h ethanol productivity. Mannanase showed the lowest performance with 79 % saccharification and 0.21 g/L/h ethanol productivity. A synergistic enzyme cocktail maximized substrate breakdown and sugar release. This research underscores the critical role of pretreatment and enzyme selection in advancing bioethanol production, offering a sustainable pathway to valorize agricultural waste into clean energy. |
| format | Article |
| id | doaj-art-4c7f8a7ab5624c82ae881b0c724d5aaa |
| institution | OA Journals |
| issn | 2949-8392 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sustainable Chemistry for the Environment |
| spelling | doaj-art-4c7f8a7ab5624c82ae881b0c724d5aaa2025-08-20T02:08:38ZengElsevierSustainable Chemistry for the Environment2949-83922025-06-011010023910.1016/j.scenv.2025.100239Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol productionSudarshan Sahu0Urbi Bansal1Gursharan Singh2Shailendra Kumar Arya3Department of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, IndiaDepartment of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, IndiaDepartment of Medical Laboratory Sciences, Lovely Professional University, Phagwara, Punjab 144411, IndiaDepartment of Biotechnology, University Institute of Engineering and Technology, Panjab University, Chandigarh, India; Corresponding author.The global shift towards renewable energy has heightened the importance of bioethanol as a sustainable alternative to fossil fuels, addressing environmental concerns and reducing greenhouse gas emissions. Sustainable chemistry offers innovative solutions for converting agricultural residues into valuable biofuels, yet challenges in optimizing pretreatment and enzymatic processes persist. This study addresses these gaps by systematically comparing physical, chemical, physicochemical, and enzymatic pretreatments to enhance ethanol yields from paddy straw. Methods included alkali, sonication, and alkali-assisted sonication treatments to modify substrate composition, followed by enzymatic hydrolysis using cellulase, xylanase, and mannanase. Results revealed that alkali-assisted sonication yielded the highest reducing sugar concentrations (30 ± 0.8 mg/mL) and ethanol productivity (0.41 g/L/h), with a saccharification percentage of 89 % and ethanol yield of 0.58 g/L. In contrast, xylanase exhibited a saccharification percentage of 83 % with an ethanol productivity of 0.28 g/L/h, while cellulase achieved 85 % saccharification and 0.35 g/L/h ethanol productivity. Mannanase showed the lowest performance with 79 % saccharification and 0.21 g/L/h ethanol productivity. A synergistic enzyme cocktail maximized substrate breakdown and sugar release. This research underscores the critical role of pretreatment and enzyme selection in advancing bioethanol production, offering a sustainable pathway to valorize agricultural waste into clean energy.http://www.sciencedirect.com/science/article/pii/S2949839225000343BioethanolFermentationPaddy strawPretreatmentLignocellulosic biomassEnzymatic hydrolysis |
| spellingShingle | Sudarshan Sahu Urbi Bansal Gursharan Singh Shailendra Kumar Arya Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production Sustainable Chemistry for the Environment Bioethanol Fermentation Paddy straw Pretreatment Lignocellulosic biomass Enzymatic hydrolysis |
| title | Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production |
| title_full | Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production |
| title_fullStr | Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production |
| title_full_unstemmed | Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production |
| title_short | Comparison of physical, chemical, physico-chemical, and enzymatic pretreatment of paddy straw for ethanol production |
| title_sort | comparison of physical chemical physico chemical and enzymatic pretreatment of paddy straw for ethanol production |
| topic | Bioethanol Fermentation Paddy straw Pretreatment Lignocellulosic biomass Enzymatic hydrolysis |
| url | http://www.sciencedirect.com/science/article/pii/S2949839225000343 |
| work_keys_str_mv | AT sudarshansahu comparisonofphysicalchemicalphysicochemicalandenzymaticpretreatmentofpaddystrawforethanolproduction AT urbibansal comparisonofphysicalchemicalphysicochemicalandenzymaticpretreatmentofpaddystrawforethanolproduction AT gursharansingh comparisonofphysicalchemicalphysicochemicalandenzymaticpretreatmentofpaddystrawforethanolproduction AT shailendrakumararya comparisonofphysicalchemicalphysicochemicalandenzymaticpretreatmentofpaddystrawforethanolproduction |