Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients

ObjectivesLittle is known about how various treatments impact the progression of interstitial lung disease (ILD) in rheumatoid arthritis (RA) patients. Here, we compared ILD progression in RA patients treated with Janus kinase inhibitors (JAKi) or biological disease-modifying anti-rheumatic drugs (b...

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Main Authors: Shota Kurushima, Tomohiro Koga, Masataka Umeda, Naoki Iwamoto, Ritsuko Miyashita, Takatomo Tokito, Daisuke Okuno, Hirokazu Yura, Hiroshi Ishimoto, Takashi Kido, Noriho Sakamoto, Yukitaka Ueki, Hiroshi Mukae, Atsushi Kawakami
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Immunology
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Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1501146/full
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author Shota Kurushima
Tomohiro Koga
Masataka Umeda
Naoki Iwamoto
Ritsuko Miyashita
Takatomo Tokito
Daisuke Okuno
Hirokazu Yura
Hiroshi Ishimoto
Takashi Kido
Noriho Sakamoto
Yukitaka Ueki
Hiroshi Mukae
Atsushi Kawakami
author_facet Shota Kurushima
Tomohiro Koga
Masataka Umeda
Naoki Iwamoto
Ritsuko Miyashita
Takatomo Tokito
Daisuke Okuno
Hirokazu Yura
Hiroshi Ishimoto
Takashi Kido
Noriho Sakamoto
Yukitaka Ueki
Hiroshi Mukae
Atsushi Kawakami
author_sort Shota Kurushima
collection DOAJ
description ObjectivesLittle is known about how various treatments impact the progression of interstitial lung disease (ILD) in rheumatoid arthritis (RA) patients. Here, we compared ILD progression in RA patients treated with Janus kinase inhibitors (JAKi) or biological disease-modifying anti-rheumatic drugs (bDMARDs). In vitro experiments were also performed to evaluate the potential effects of the drugs on epithelial–mesenchymal transition (EMT), a key event in pulmonary fibrosis.MethodsThis retrospective study included 93 RA-ILD patients who initiated treatment with JAKi, tumour necrosis factor inhibitors (TNFi), or abatacept between 2017 and 2020. Worsening ILD was quantified by changes in chest computed tomography (CT) scans between baseline and follow-up (mean 14 months, range 6–51 months). Response to treatment was evaluated using Disease Activity Score-28 with erythrocyte sedimentation rate (DAS28-ESR). Expression of the EMT marker N-cadherin in A549 lung cells was assessed by western blotting.Results and discussionWorsening ILD was detected in 19.4% (7/36), 16.7% (5/30), and 22.2% (6/27) of patients treated with JAKi, abatacept, and TNFi, respectively. Multivariate analysis identified female gender (P=0.043) and >10% fibrotic lesions (P=0.015) as significant predictors of worsening ILD. DAS28-ESR-based non-responder status was also significantly associated with worsening ILD (P=0.0085). In vitro, combination treatment with methotrexate and baricitinib significantly impeded EMT progression. Worsening ILD was associated with more extensive fibrotic lesions at baseline and female gender in RA patients treated with JAKi or bDMARDs. JAKi and methotrexate co-treatment may prove beneficial in modifying key events underlying the pathogenesis of RA-ILD.
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spelling doaj-art-4c7df43ecc684344a1dba6eae5a191882025-08-20T02:35:53ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-12-011510.3389/fimmu.2024.15011461501146Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patientsShota Kurushima0Tomohiro Koga1Masataka Umeda2Naoki Iwamoto3Ritsuko Miyashita4Takatomo Tokito5Daisuke Okuno6Hirokazu Yura7Hiroshi Ishimoto8Takashi Kido9Noriho Sakamoto10Yukitaka Ueki11Hiroshi Mukae12Atsushi Kawakami13Department of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanRheumatic Disease Centre, Sasebo Chuo Hospital, Sasebo, JapanDepartment of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanObjectivesLittle is known about how various treatments impact the progression of interstitial lung disease (ILD) in rheumatoid arthritis (RA) patients. Here, we compared ILD progression in RA patients treated with Janus kinase inhibitors (JAKi) or biological disease-modifying anti-rheumatic drugs (bDMARDs). In vitro experiments were also performed to evaluate the potential effects of the drugs on epithelial–mesenchymal transition (EMT), a key event in pulmonary fibrosis.MethodsThis retrospective study included 93 RA-ILD patients who initiated treatment with JAKi, tumour necrosis factor inhibitors (TNFi), or abatacept between 2017 and 2020. Worsening ILD was quantified by changes in chest computed tomography (CT) scans between baseline and follow-up (mean 14 months, range 6–51 months). Response to treatment was evaluated using Disease Activity Score-28 with erythrocyte sedimentation rate (DAS28-ESR). Expression of the EMT marker N-cadherin in A549 lung cells was assessed by western blotting.Results and discussionWorsening ILD was detected in 19.4% (7/36), 16.7% (5/30), and 22.2% (6/27) of patients treated with JAKi, abatacept, and TNFi, respectively. Multivariate analysis identified female gender (P=0.043) and >10% fibrotic lesions (P=0.015) as significant predictors of worsening ILD. DAS28-ESR-based non-responder status was also significantly associated with worsening ILD (P=0.0085). In vitro, combination treatment with methotrexate and baricitinib significantly impeded EMT progression. Worsening ILD was associated with more extensive fibrotic lesions at baseline and female gender in RA patients treated with JAKi or bDMARDs. JAKi and methotrexate co-treatment may prove beneficial in modifying key events underlying the pathogenesis of RA-ILD.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1501146/fullrheumatoid arthritisinterstitial lung diseaseRA-ILDJAK inhibitorsmethotrexateepithelial-mesenchymal transition
spellingShingle Shota Kurushima
Tomohiro Koga
Masataka Umeda
Naoki Iwamoto
Ritsuko Miyashita
Takatomo Tokito
Daisuke Okuno
Hirokazu Yura
Hiroshi Ishimoto
Takashi Kido
Noriho Sakamoto
Yukitaka Ueki
Hiroshi Mukae
Atsushi Kawakami
Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
Frontiers in Immunology
rheumatoid arthritis
interstitial lung disease
RA-ILD
JAK inhibitors
methotrexate
epithelial-mesenchymal transition
title Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
title_full Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
title_fullStr Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
title_full_unstemmed Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
title_short Impact of Janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
title_sort impact of janus kinase inhibitors and methotrexate on interstitial lung disease in rheumatoid arthritis patients
topic rheumatoid arthritis
interstitial lung disease
RA-ILD
JAK inhibitors
methotrexate
epithelial-mesenchymal transition
url https://www.frontiersin.org/articles/10.3389/fimmu.2024.1501146/full
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