The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly
A complex composed of Pba3-Pba4 and subunits α5, α6, and α7 is identified as an early intermediate in proteasome core particle assembly in wild-type <i>Saccharomyces cerevisiae</i> cell<i>s</i>. The same complex can be reconstituted from recombinantly produced components in v...
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MDPI AG
2025-05-01
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| Series: | Biomolecules |
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| author | Ana C. Matias Margarida N. Tiago Jessica Zimmermann R. Jürgen Dohmen Paula C. Ramos |
| author_facet | Ana C. Matias Margarida N. Tiago Jessica Zimmermann R. Jürgen Dohmen Paula C. Ramos |
| author_sort | Ana C. Matias |
| collection | DOAJ |
| description | A complex composed of Pba3-Pba4 and subunits α5, α6, and α7 is identified as an early intermediate in proteasome core particle assembly in wild-type <i>Saccharomyces cerevisiae</i> cell<i>s</i>. The same complex can be reconstituted from recombinantly produced components in vitro. Assembly of α6 and α7 with Pba3-Pba4 depends on the presence of the α5 subunit, the binding of which apparently initiates the formation of this intermediate. Our data suggest the following order of events: first, Pba3-Pba4 binds α5, then α6 is incorporated, and at the end α7. In the absence of the chaperones Pba1-Pba2 or Ump1, alternative Pba4-containing complexes are detected, the formation of which depends on the Blm10/PA200 protein. Overexpression of Pba1-Pba2 abolishes the formation of these complexes containing Pba4 and Blm10, suggesting that Blm10 may replace Pba1-Pba2 as an alternative assembly factor. |
| format | Article |
| id | doaj-art-4c7911df25d6445c8f9a3e44da10bc84 |
| institution | DOAJ |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-4c7911df25d6445c8f9a3e44da10bc842025-08-20T03:14:45ZengMDPI AGBiomolecules2218-273X2025-05-0115568310.3390/biom15050683The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle AssemblyAna C. Matias0Margarida N. Tiago1Jessica Zimmermann2R. Jürgen Dohmen3Paula C. Ramos4Center of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, GermanyCenter of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, GermanyCenter of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, GermanyCenter of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, GermanyCenter of Molecular Biosciences, Institute for Genetics, Department of Biology, Faculty of Natural Sciences and Mathematics, University of Cologne, 50674 Cologne, GermanyA complex composed of Pba3-Pba4 and subunits α5, α6, and α7 is identified as an early intermediate in proteasome core particle assembly in wild-type <i>Saccharomyces cerevisiae</i> cell<i>s</i>. The same complex can be reconstituted from recombinantly produced components in vitro. Assembly of α6 and α7 with Pba3-Pba4 depends on the presence of the α5 subunit, the binding of which apparently initiates the formation of this intermediate. Our data suggest the following order of events: first, Pba3-Pba4 binds α5, then α6 is incorporated, and at the end α7. In the absence of the chaperones Pba1-Pba2 or Ump1, alternative Pba4-containing complexes are detected, the formation of which depends on the Blm10/PA200 protein. Overexpression of Pba1-Pba2 abolishes the formation of these complexes containing Pba4 and Blm10, suggesting that Blm10 may replace Pba1-Pba2 as an alternative assembly factor.https://www.mdpi.com/2218-273X/15/5/683proteasome core particleassemblychaperonesPba3-Pba4Blm10 |
| spellingShingle | Ana C. Matias Margarida N. Tiago Jessica Zimmermann R. Jürgen Dohmen Paula C. Ramos The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly Biomolecules proteasome core particle assembly chaperones Pba3-Pba4 Blm10 |
| title | The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly |
| title_full | The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly |
| title_fullStr | The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly |
| title_full_unstemmed | The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly |
| title_short | The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly |
| title_sort | α5 α6 α7 pba3 pba4 complex a starting unit in proteasome core particle assembly |
| topic | proteasome core particle assembly chaperones Pba3-Pba4 Blm10 |
| url | https://www.mdpi.com/2218-273X/15/5/683 |
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