Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model

Chikungunya virus (CHIKV) is an alphavirus transmitted by mosquitos that poses a threat to global public health and for which there is an urgent need for widespread access to globally licensed vaccines. Here, we demonstrate that an inactivated CHIKV vaccine (BBV87) protects against systemic infectio...

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Main Authors: Sarah L. Kempster, Deborah Ferguson, Claire Ham, Joanna Hall, Adrian Jenkins, Elaine Giles, Simon L. Priestnall, Alejandro Suarez-Bonnet, Pierre Roques, Roger Le Grand, Sumathy Kandaswamy, Sushant Sahastrabuddhe, Libia Milena Hernandez, Sunee Chuasuwan, Hyeon Seon Ahn, Deok Ryun Kim, Anh Wartel, Raphaël M. Zellweger, Neil Berry, Neil Almond
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Language:English
Published: MDPI AG 2025-04-01
Series:Viruses
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Online Access:https://www.mdpi.com/1999-4915/17/4/550
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author Sarah L. Kempster
Deborah Ferguson
Claire Ham
Joanna Hall
Adrian Jenkins
Elaine Giles
Simon L. Priestnall
Alejandro Suarez-Bonnet
Pierre Roques
Roger Le Grand
Sumathy Kandaswamy
Sushant Sahastrabuddhe
Libia Milena Hernandez
Sunee Chuasuwan
Hyeon Seon Ahn
Deok Ryun Kim
Anh Wartel
Raphaël M. Zellweger
Neil Berry
Neil Almond
author_facet Sarah L. Kempster
Deborah Ferguson
Claire Ham
Joanna Hall
Adrian Jenkins
Elaine Giles
Simon L. Priestnall
Alejandro Suarez-Bonnet
Pierre Roques
Roger Le Grand
Sumathy Kandaswamy
Sushant Sahastrabuddhe
Libia Milena Hernandez
Sunee Chuasuwan
Hyeon Seon Ahn
Deok Ryun Kim
Anh Wartel
Raphaël M. Zellweger
Neil Berry
Neil Almond
author_sort Sarah L. Kempster
collection DOAJ
description Chikungunya virus (CHIKV) is an alphavirus transmitted by mosquitos that poses a threat to global public health and for which there is an urgent need for widespread access to globally licensed vaccines. Here, we demonstrate that an inactivated CHIKV vaccine (BBV87) protects against systemic infection with CHIKV in a non-human primate (NHP) challenge model. Groups of five cynomolgus macaques received two doses of 20 µg BBV87 vaccine or saline alone (28 days apart). Twenty-eight days after the second immunisation, all animals were challenged with CHIKV. All controls were productively infected with detectable viremia and pathological responses following challenge, including altered thermoregulation, haematological and cytokine changes. Critically, the histopathological analysis of finger joints identified areas of inflammation in the synovium. By contrast vaccinated macaques had no detectable viremia and none of the pathological changes were reported in control animals. This study demonstrates that a 20 µg dose of BBV87 vaccine confers robust protection in vivo, both on the acquisition of infection and pathology.
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spelling doaj-art-4c78d0fc7568447985e7c80ce5cf02f02025-08-20T02:18:11ZengMDPI AGViruses1999-49152025-04-0117455010.3390/v17040550Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate ModelSarah L. Kempster0Deborah Ferguson1Claire Ham2Joanna Hall3Adrian Jenkins4Elaine Giles5Simon L. Priestnall6Alejandro Suarez-Bonnet7Pierre Roques8Roger Le Grand9Sumathy Kandaswamy10Sushant Sahastrabuddhe11Libia Milena Hernandez12Sunee Chuasuwan13Hyeon Seon Ahn14Deok Ryun Kim15Anh Wartel16Raphaël M. Zellweger17Neil Berry18Neil Almond19Science and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKDepartment Pathobiology & Population Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield AL9 7TA, Hertfordshire, UKDepartment Pathobiology & Population Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield AL9 7TA, Hertfordshire, UKINSERM, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB/IDMIT), Université Paris-Saclay, 92260 Fontenay-aux-Roses, FranceINSERM, CEA, Center for Immunology of Viral, Auto-Immune, Hematological and Bacterial Diseases (IMVA-HB/IDMIT), Université Paris-Saclay, 92260 Fontenay-aux-Roses, FranceBharat Biotech International Ltd., Hyderabad 500078, IndiaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaInternational Vaccine Institute (IVI), Seoul 08800, Republic of KoreaScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKScience and Research, Diagnostics, Medicines and Healthcare Products Regulatory Agency, Blanche Lane, South Mimms EN6 3QG, Hertfordshire, UKChikungunya virus (CHIKV) is an alphavirus transmitted by mosquitos that poses a threat to global public health and for which there is an urgent need for widespread access to globally licensed vaccines. Here, we demonstrate that an inactivated CHIKV vaccine (BBV87) protects against systemic infection with CHIKV in a non-human primate (NHP) challenge model. Groups of five cynomolgus macaques received two doses of 20 µg BBV87 vaccine or saline alone (28 days apart). Twenty-eight days after the second immunisation, all animals were challenged with CHIKV. All controls were productively infected with detectable viremia and pathological responses following challenge, including altered thermoregulation, haematological and cytokine changes. Critically, the histopathological analysis of finger joints identified areas of inflammation in the synovium. By contrast vaccinated macaques had no detectable viremia and none of the pathological changes were reported in control animals. This study demonstrates that a 20 µg dose of BBV87 vaccine confers robust protection in vivo, both on the acquisition of infection and pathology.https://www.mdpi.com/1999-4915/17/4/550chikungunya virusvaccinenon-human primatepathogenesisimmunity
spellingShingle Sarah L. Kempster
Deborah Ferguson
Claire Ham
Joanna Hall
Adrian Jenkins
Elaine Giles
Simon L. Priestnall
Alejandro Suarez-Bonnet
Pierre Roques
Roger Le Grand
Sumathy Kandaswamy
Sushant Sahastrabuddhe
Libia Milena Hernandez
Sunee Chuasuwan
Hyeon Seon Ahn
Deok Ryun Kim
Anh Wartel
Raphaël M. Zellweger
Neil Berry
Neil Almond
Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model
Viruses
chikungunya virus
vaccine
non-human primate
pathogenesis
immunity
title Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model
title_full Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model
title_fullStr Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model
title_full_unstemmed Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model
title_short Inactivated Viral Vaccine BBV87 Protects Against Chikungunya Virus Challenge in a Non-Human Primate Model
title_sort inactivated viral vaccine bbv87 protects against chikungunya virus challenge in a non human primate model
topic chikungunya virus
vaccine
non-human primate
pathogenesis
immunity
url https://www.mdpi.com/1999-4915/17/4/550
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