Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice
Abstract Close coordination of carbon and nitrogen (C/N) metabolism is necessary to maintain optimal growth and development of plants and other cellular organisms. The central regulator for achieving high‐yield and quality synergy in rice through regulating the dynamic C/N balance has seldom been re...
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| Format: | Article |
| Language: | English |
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Wiley
2025-08-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202504163 |
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| author | Guangming Lou Pingli Chen Pingbo Li Haozhou Gao Jiawang Xiong Shanshan Wan Yuanyuan Zheng Yufu Wang Mufid Alam Yingnanjun Chen Lei Wang Jingjing Bai Xuan Tan Wenting Rao Bian Wu Hao Zhou Yanhua Li Guanjun Gao Qinglu Zhang Jinghua Xiao Xianghua Li Xuelei lai Qifa Zhang Yuqing He |
| author_facet | Guangming Lou Pingli Chen Pingbo Li Haozhou Gao Jiawang Xiong Shanshan Wan Yuanyuan Zheng Yufu Wang Mufid Alam Yingnanjun Chen Lei Wang Jingjing Bai Xuan Tan Wenting Rao Bian Wu Hao Zhou Yanhua Li Guanjun Gao Qinglu Zhang Jinghua Xiao Xianghua Li Xuelei lai Qifa Zhang Yuqing He |
| author_sort | Guangming Lou |
| collection | DOAJ |
| description | Abstract Close coordination of carbon and nitrogen (C/N) metabolism is necessary to maintain optimal growth and development of plants and other cellular organisms. The central regulator for achieving high‐yield and quality synergy in rice through regulating the dynamic C/N balance has seldom been reported. Here, the novel function of Grain number, plant height, and heading date7 (Ghd7) gene are reported as both a negative regulator of grain protein content and a positive regulator of rice grain quality (including appearance quality and eating quality). As a transcription factor with both activating and inhibitory functions, Ghd7 directly binds to CCACC motif genes involved in C/N metabolism. OsNAC42, an interacting protein of Ghd7, antagonistically regulates the expression of these target genes in rice seedlings and endosperm by forming a heterodimer with Ghd7. The antagonistic Ghd7‐OsNAC42 module can flexibly regulate C/N metabolism in vivo in response to various nitrogen levels, thereby maintaining a dynamic C/N balance. Phenotypically, OsNAC42 enhances grain protein content but compromises quality‐functions opposing Ghd7's effects. In summary, the discovery of the Ghd7‐OsNAC42 antagonistic module provides new insights into the synergy between superior quality and high yield in rice. |
| format | Article |
| id | doaj-art-4c5f1b43d1b64780b3065205a173e893 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-4c5f1b43d1b64780b3065205a173e8932025-08-23T14:13:49ZengWileyAdvanced Science2198-38442025-08-011231n/an/a10.1002/advs.202504163Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in RiceGuangming Lou0Pingli Chen1Pingbo Li2Haozhou Gao3Jiawang Xiong4Shanshan Wan5Yuanyuan Zheng6Yufu Wang7Mufid Alam8Yingnanjun Chen9Lei Wang10Jingjing Bai11Xuan Tan12Wenting Rao13Bian Wu14Hao Zhou15Yanhua Li16Guanjun Gao17Qinglu Zhang18Jinghua Xiao19Xianghua Li20Xuelei lai21Qifa Zhang22Yuqing He23National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Hubei Hongshan Laboratory Huazhong Agricultural University Wuhan 430070 ChinaAbstract Close coordination of carbon and nitrogen (C/N) metabolism is necessary to maintain optimal growth and development of plants and other cellular organisms. The central regulator for achieving high‐yield and quality synergy in rice through regulating the dynamic C/N balance has seldom been reported. Here, the novel function of Grain number, plant height, and heading date7 (Ghd7) gene are reported as both a negative regulator of grain protein content and a positive regulator of rice grain quality (including appearance quality and eating quality). As a transcription factor with both activating and inhibitory functions, Ghd7 directly binds to CCACC motif genes involved in C/N metabolism. OsNAC42, an interacting protein of Ghd7, antagonistically regulates the expression of these target genes in rice seedlings and endosperm by forming a heterodimer with Ghd7. The antagonistic Ghd7‐OsNAC42 module can flexibly regulate C/N metabolism in vivo in response to various nitrogen levels, thereby maintaining a dynamic C/N balance. Phenotypically, OsNAC42 enhances grain protein content but compromises quality‐functions opposing Ghd7's effects. In summary, the discovery of the Ghd7‐OsNAC42 antagonistic module provides new insights into the synergy between superior quality and high yield in rice.https://doi.org/10.1002/advs.202504163carbon and nitrogen metabolismGhd7grain qualityOsNAC42protein contentrice |
| spellingShingle | Guangming Lou Pingli Chen Pingbo Li Haozhou Gao Jiawang Xiong Shanshan Wan Yuanyuan Zheng Yufu Wang Mufid Alam Yingnanjun Chen Lei Wang Jingjing Bai Xuan Tan Wenting Rao Bian Wu Hao Zhou Yanhua Li Guanjun Gao Qinglu Zhang Jinghua Xiao Xianghua Li Xuelei lai Qifa Zhang Yuqing He Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice Advanced Science carbon and nitrogen metabolism Ghd7 grain quality OsNAC42 protein content rice |
| title | Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice |
| title_full | Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice |
| title_fullStr | Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice |
| title_full_unstemmed | Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice |
| title_short | Antagonistic Ghd7‐OsNAC42 Complexes Modulate Carbon and Nitrogen Metabolism to Achieves Superior Quality and High Yield in Rice |
| title_sort | antagonistic ghd7 osnac42 complexes modulate carbon and nitrogen metabolism to achieves superior quality and high yield in rice |
| topic | carbon and nitrogen metabolism Ghd7 grain quality OsNAC42 protein content rice |
| url | https://doi.org/10.1002/advs.202504163 |
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