Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification
Dangdang Wang,1,2,* Yanyu Pu,1,2,* Xi Gao,1,2 Lihong Zeng,1,2 Hong Li1,2 1Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Ey...
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Dove Medical Press
2025-01-01
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| Series: | Journal of Inflammation Research |
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| author | Wang D Pu Y Gao X Zeng L Li H |
| author_facet | Wang D Pu Y Gao X Zeng L Li H |
| author_sort | Wang D |
| collection | DOAJ |
| description | Dangdang Wang,1,2,* Yanyu Pu,1,2,* Xi Gao,1,2 Lihong Zeng,1,2 Hong Li1,2 1Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong Li, Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China, Email lihong@hospital.cqmu.edu.cnPurpose: Genome-wide association studies (GWAS) have identified multiple genetic loci associated with primary open-angle glaucoma (POAG). However, the mechanisms by which these loci contribute to POAG progression remain unclear. This study aimed to identify potential causative genes involved in the development of POAG.Methods: We utilized multi-dimensional high-throughput data, integrating proteome-wide association study(PWAS), transcriptome-wide association study (TWAS), and summary data-based Mendelian randomization (SMR) analysis. This approach enabled the identification of genes influencing POAG risk by affecting gene expression and protein concentrations in the bloodstream. The key gene was validated through enzyme-linked immunosorbent assay (ELISA) analysis.Results: PWAS identified 86 genes associated with altered blood protein levels in POAG patients. Of these, eight genes (SFTPD, CSK, COL18A1, TCN2, GZMK, RAB2A, TEK, and GNLY) were identified as likely causative for POAG (PSMR |
| format | Article |
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| institution | Kabale University |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj-art-4c56834cabb242f49bd89b2bd73dae092025-01-09T16:58:34ZengDove Medical PressJournal of Inflammation Research1178-70312025-01-01Volume 1836738099076Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental VerificationWang DPu YGao XZeng LLi HDangdang Wang,1,2,* Yanyu Pu,1,2,* Xi Gao,1,2 Lihong Zeng,1,2 Hong Li1,2 1Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China; 2Chongqing Key Laboratory for the Prevention and Treatment of Major Blinding Eye Diseases, Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, Chongqing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Hong Li, Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People’s Republic of China, Email lihong@hospital.cqmu.edu.cnPurpose: Genome-wide association studies (GWAS) have identified multiple genetic loci associated with primary open-angle glaucoma (POAG). However, the mechanisms by which these loci contribute to POAG progression remain unclear. This study aimed to identify potential causative genes involved in the development of POAG.Methods: We utilized multi-dimensional high-throughput data, integrating proteome-wide association study(PWAS), transcriptome-wide association study (TWAS), and summary data-based Mendelian randomization (SMR) analysis. This approach enabled the identification of genes influencing POAG risk by affecting gene expression and protein concentrations in the bloodstream. The key gene was validated through enzyme-linked immunosorbent assay (ELISA) analysis.Results: PWAS identified 86 genes associated with altered blood protein levels in POAG patients. Of these, eight genes (SFTPD, CSK, COL18A1, TCN2, GZMK, RAB2A, TEK, and GNLY) were identified as likely causative for POAG (PSMRhttps://www.dovepress.com/potential-functions-and-causal-associations-of-gnly-in-primary-open-an-peer-reviewed-fulltext-article-JIRprimary open-angle glaucomagnlypwastwas |
| spellingShingle | Wang D Pu Y Gao X Zeng L Li H Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification Journal of Inflammation Research primary open-angle glaucoma gnly pwas twas |
| title | Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification |
| title_full | Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification |
| title_fullStr | Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification |
| title_full_unstemmed | Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification |
| title_short | Potential Functions and Causal Associations of GNLY in Primary Open-Angle Glaucoma: Integration of Blood-Derived Proteome, Transcriptome, and Experimental Verification |
| title_sort | potential functions and causal associations of gnly in primary open angle glaucoma integration of blood derived proteome transcriptome and experimental verification |
| topic | primary open-angle glaucoma gnly pwas twas |
| url | https://www.dovepress.com/potential-functions-and-causal-associations-of-gnly-in-primary-open-an-peer-reviewed-fulltext-article-JIR |
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