Role of ferroptosis in acute cadmium-induced liver injury in mice
Objective To investigate the role of ferroptosis in acute liver injury induced by cadmium (Cd) exposure in mice. Methods Healthy and clean male ICR mice of 8-10 weeks were randomly divided into control group, and Cd exposure group. The mice in the Cd exposure group were injected with an equal amount...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Guangxi Medical University
2024-12-01
|
| Series: | Guangxi Yike Daxue xuebao |
| Subjects: | |
| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.12.002 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850276226320564224 |
|---|---|
| author | YIN Qizi TANG Yuting ZHANG Saijun GUO Siyu LI Jing WANG Ziyue JI Yanli |
| author_facet | YIN Qizi TANG Yuting ZHANG Saijun GUO Siyu LI Jing WANG Ziyue JI Yanli |
| author_sort | YIN Qizi |
| collection | DOAJ |
| description | Objective To investigate the role of ferroptosis in acute liver injury induced by cadmium (Cd) exposure in mice. Methods Healthy and clean male ICR mice of 8-10 weeks were randomly divided into control group, and Cd exposure group. The mice in the Cd exposure group were injected with an equal amount of CdCl2 (4 mg·kg-1) intraperitoneally. Liver tissues and blood were collected after Cd treatment for 6h, 12h and 24h, respectively. The mice in the control group were injected with normal saline in the same way. The hematoxylineosin (HE) staining method was used to observe the pathological injury of liver. The contents of ALT, AST, total iron, GSH, SOD, H2O2 and MDA in serum were detected by corresponding kits. The expression of key genes of ferroptosis in liver tissues of mice were detected by real-time fluorescence quantitative PCR (RT-qPCR) and western blotting. Results The acute Cd exposure reduced liver weight and organ coefficient in mice, and significantly caused liver function and pathological damage. Compared with the control group, the contents of GSH and SOD in the Cd exposure group were decreased, the contents of ALT, AST, and MDA were increased, and the content of H2O2 was significantly increased (P < 0.05). In addition, Cd significantly increased the total iron content in liver of mice, induced the protein expression of HO-1 and FTH1 in liver tissue of mice, while the protein expression of GPX4 was significantly decreased in the Cd exposure group (P < 0.05). Conclusion Acute Cd exposure can cause oxidative stress, increase iron content, and alter transcription and protein expression of genes associated with ferroptosis in the liver, suggesting that ferroptosis may be involved in liver injury induced by acute Cd exposure. |
| format | Article |
| id | doaj-art-4c5196187d814598bf9abfc522cfd16e |
| institution | OA Journals |
| issn | 1005-930X |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
| record_format | Article |
| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-4c5196187d814598bf9abfc522cfd16e2025-08-20T01:50:22ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2024-12-0141121588159410.16190/j.cnki.45-1211/r.2024.12.002gxykdxxb-41-12-1588Role of ferroptosis in acute cadmium-induced liver injury in miceYIN Qizi0TANG Yuting1ZHANG Saijun2GUO Siyu3LI Jing4WANG Ziyue5JI Yanli6Department of Health Inspection and Quarantine, School of Public Health, Anhui Medical University, Hefei 230032, ChinaThe First Clinical School of Medicine, Anhui Medical University, Hefei 230032, ChinaThe First Clinical School of Medicine, Anhui Medical University, Hefei 230032, ChinaThe First Clinical School of Medicine, Anhui Medical University, Hefei 230032, ChinaThe First Clinical School of Medicine, Anhui Medical University, Hefei 230032, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Anhui Medical University, Hefei 230032, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Anhui Medical University, Hefei 230032, ChinaObjective To investigate the role of ferroptosis in acute liver injury induced by cadmium (Cd) exposure in mice. Methods Healthy and clean male ICR mice of 8-10 weeks were randomly divided into control group, and Cd exposure group. The mice in the Cd exposure group were injected with an equal amount of CdCl2 (4 mg·kg-1) intraperitoneally. Liver tissues and blood were collected after Cd treatment for 6h, 12h and 24h, respectively. The mice in the control group were injected with normal saline in the same way. The hematoxylineosin (HE) staining method was used to observe the pathological injury of liver. The contents of ALT, AST, total iron, GSH, SOD, H2O2 and MDA in serum were detected by corresponding kits. The expression of key genes of ferroptosis in liver tissues of mice were detected by real-time fluorescence quantitative PCR (RT-qPCR) and western blotting. Results The acute Cd exposure reduced liver weight and organ coefficient in mice, and significantly caused liver function and pathological damage. Compared with the control group, the contents of GSH and SOD in the Cd exposure group were decreased, the contents of ALT, AST, and MDA were increased, and the content of H2O2 was significantly increased (P < 0.05). In addition, Cd significantly increased the total iron content in liver of mice, induced the protein expression of HO-1 and FTH1 in liver tissue of mice, while the protein expression of GPX4 was significantly decreased in the Cd exposure group (P < 0.05). Conclusion Acute Cd exposure can cause oxidative stress, increase iron content, and alter transcription and protein expression of genes associated with ferroptosis in the liver, suggesting that ferroptosis may be involved in liver injury induced by acute Cd exposure.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.12.002ferroptosiscadmiumliveroxidative stress |
| spellingShingle | YIN Qizi TANG Yuting ZHANG Saijun GUO Siyu LI Jing WANG Ziyue JI Yanli Role of ferroptosis in acute cadmium-induced liver injury in mice Guangxi Yike Daxue xuebao ferroptosis cadmium liver oxidative stress |
| title | Role of ferroptosis in acute cadmium-induced liver injury in mice |
| title_full | Role of ferroptosis in acute cadmium-induced liver injury in mice |
| title_fullStr | Role of ferroptosis in acute cadmium-induced liver injury in mice |
| title_full_unstemmed | Role of ferroptosis in acute cadmium-induced liver injury in mice |
| title_short | Role of ferroptosis in acute cadmium-induced liver injury in mice |
| title_sort | role of ferroptosis in acute cadmium induced liver injury in mice |
| topic | ferroptosis cadmium liver oxidative stress |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.12.002 |
| work_keys_str_mv | AT yinqizi roleofferroptosisinacutecadmiuminducedliverinjuryinmice AT tangyuting roleofferroptosisinacutecadmiuminducedliverinjuryinmice AT zhangsaijun roleofferroptosisinacutecadmiuminducedliverinjuryinmice AT guosiyu roleofferroptosisinacutecadmiuminducedliverinjuryinmice AT lijing roleofferroptosisinacutecadmiuminducedliverinjuryinmice AT wangziyue roleofferroptosisinacutecadmiuminducedliverinjuryinmice AT jiyanli roleofferroptosisinacutecadmiuminducedliverinjuryinmice |