Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells
Introduction. Many pathological conditions may benefit from cell therapy using mesenchymal stromal cells, particularly from adipose tissue (ASCs). Cells may be grafted in an environment with a remnant polymicrobial component. The aim is to investigate the behavior of ASCs when brought in contact wit...
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| Format: | Article |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5389629 |
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| author | Paul Monsarrat Philippe Kémoun Louis Casteilla Valérie Planat-Bénard |
| author_facet | Paul Monsarrat Philippe Kémoun Louis Casteilla Valérie Planat-Bénard |
| author_sort | Paul Monsarrat |
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| description | Introduction. Many pathological conditions may benefit from cell therapy using mesenchymal stromal cells, particularly from adipose tissue (ASCs). Cells may be grafted in an environment with a remnant polymicrobial component. The aim is to investigate the behavior of ASCs when brought in contact with a large panel of bacteria. Materials and Methods. Carboxyfluorescein-labelled bacterial interaction with ASCs was followed by confocal time-lapse microscopy. Costaining with LAMP-1 was also analyzed. Viability of 4 gram-negative and 4 gram-positive bacterial strains after 6 h of coculture with ASCs was assessed by agar colony counting and by flow cytometry using SYTO-62®/propidium iodide (PI) for membrane permeabilization and DiOC6 for depolarization. A murine model of periodontitis was used to assess in vivo antibacterial capacities of ASCs. Results. A significant increase of PI-positive events for all bacterial strains and an increase of the DiOC6 signal were obtained after contact with ASCs. The number of CFU was also significantly decreased for several bacterial strains. 0.4 μm transwell systems illustrated the necessary direct contact to induce maximal bacterial membrane damages. Some bacteria were observed into phagolysosomes, confirming macrophage-like properties of ASCs. In vivo, the bacterial load was significantly lower in the ASC-grafted side compared to the control. Conclusion. Our results highlight for the first time a broad range of antibacterial actions of ASCs, by phagocytosis, secretion of oxygenated free radicals and antibacterial molecules. These data are in line with the development of new therapeutic strategies based on ASC transplantation, appropriated in immune-dysbiotic tissue context such as periodontitis or chronic wounds. |
| format | Article |
| id | doaj-art-4c4af7d093374230917ca23d2db11215 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
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| series | Stem Cells International |
| spelling | doaj-art-4c4af7d093374230917ca23d2db112152025-08-20T02:38:40ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/53896295389629Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal CellsPaul Monsarrat0Philippe Kémoun1Louis Casteilla2Valérie Planat-Bénard3The Department of Oral Rehabilitation, Paul Sabatier University, Faculty of Dentistry, Toulouse University Hospital (CHU de Toulouse), Toulouse, FranceSTROMALab, Université de Toulouse, CNRS ERL 5311, EFS, ENVT, Inserm U1031, UPS, Toulouse, FranceThe Department of Oral Surgery, Periodontology and Oral Biology, Paul Sabatier University, Faculty of Dentistry, Toulouse University Hospital (CHU de Toulouse), Toulouse, FranceThe Department of Oral Surgery, Periodontology and Oral Biology, Paul Sabatier University, Faculty of Dentistry, Toulouse University Hospital (CHU de Toulouse), Toulouse, FranceIntroduction. Many pathological conditions may benefit from cell therapy using mesenchymal stromal cells, particularly from adipose tissue (ASCs). Cells may be grafted in an environment with a remnant polymicrobial component. The aim is to investigate the behavior of ASCs when brought in contact with a large panel of bacteria. Materials and Methods. Carboxyfluorescein-labelled bacterial interaction with ASCs was followed by confocal time-lapse microscopy. Costaining with LAMP-1 was also analyzed. Viability of 4 gram-negative and 4 gram-positive bacterial strains after 6 h of coculture with ASCs was assessed by agar colony counting and by flow cytometry using SYTO-62®/propidium iodide (PI) for membrane permeabilization and DiOC6 for depolarization. A murine model of periodontitis was used to assess in vivo antibacterial capacities of ASCs. Results. A significant increase of PI-positive events for all bacterial strains and an increase of the DiOC6 signal were obtained after contact with ASCs. The number of CFU was also significantly decreased for several bacterial strains. 0.4 μm transwell systems illustrated the necessary direct contact to induce maximal bacterial membrane damages. Some bacteria were observed into phagolysosomes, confirming macrophage-like properties of ASCs. In vivo, the bacterial load was significantly lower in the ASC-grafted side compared to the control. Conclusion. Our results highlight for the first time a broad range of antibacterial actions of ASCs, by phagocytosis, secretion of oxygenated free radicals and antibacterial molecules. These data are in line with the development of new therapeutic strategies based on ASC transplantation, appropriated in immune-dysbiotic tissue context such as periodontitis or chronic wounds.http://dx.doi.org/10.1155/2019/5389629 |
| spellingShingle | Paul Monsarrat Philippe Kémoun Louis Casteilla Valérie Planat-Bénard Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells Stem Cells International |
| title | Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells |
| title_full | Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells |
| title_fullStr | Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells |
| title_full_unstemmed | Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells |
| title_short | Broad-Spectrum Antibacterial Effects of Human Adipose-Derived Stromal Cells |
| title_sort | broad spectrum antibacterial effects of human adipose derived stromal cells |
| url | http://dx.doi.org/10.1155/2019/5389629 |
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