Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure
Trace elements (TEs) in anaerobic digestion (AD) are known to be essential for optimal biogas production, but inhibitive in excessive concentrations. However, the mechanisms of inhibition are not fully understood. The effects of the addition essential TEs (Co, Cu and Ni) and a non-essential TE (Cd)...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015379 |
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| author | Rahul H.M. Pires Maria P. Tomasino Stéphane Simon Rémy Buzier Gilles Guibaud C. Marisa R. Almeida Ana P. Mucha |
| author_facet | Rahul H.M. Pires Maria P. Tomasino Stéphane Simon Rémy Buzier Gilles Guibaud C. Marisa R. Almeida Ana P. Mucha |
| author_sort | Rahul H.M. Pires |
| collection | DOAJ |
| description | Trace elements (TEs) in anaerobic digestion (AD) are known to be essential for optimal biogas production, but inhibitive in excessive concentrations. However, the mechanisms of inhibition are not fully understood. The effects of the addition essential TEs (Co, Cu and Ni) and a non-essential TE (Cd) on the microbial community structure of AD were studied in lab-scale reactors, using total TE concentrations that ranged from 0 to 100 μM. Reactor performance was assessed by monitoring biogas production. The labile fraction of TEs (the most bioaccessible species) was determined by diffusive gradients in thin-films technique. Prokaryotic community composition was characterized through high throughput sequencing (HTS) targeting archaea and bacteria and qPCR to evaluate changes in methanogens and metal resistance genes. Only a minor fraction of added TEs was labile and it decreased over time, with Ni being the most labile. Although only a minor fraction of spiked concentration was labile, all TEs inhibited biogas production at the highest spiking concentration (100 μM), with higher inhibition observed for Cd and Ni. HTS and qPCR revealed changes, particularly in archaea, with reduced relative abundance at higher TE concentrations. Shifts in prokaryotic communities suggest alterations in AD metabolic pathways. High inhibition of biogas was linked to reduced diversity, dominance of the bacterial genus Klebsiella and changes in the ratio acetoclastic / hydrogenotrophic methanogens. This study addresses a research gap in understanding how TEs inhibit AD, and provides a strategy to improve TE dosing by monitoring the labile fraction of TEs to avoid overdosing. |
| format | Article |
| id | doaj-art-4c49c7bf15d34556bc50196bcc213794 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-4c49c7bf15d34556bc50196bcc2137942025-01-23T05:25:38ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117461Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structureRahul H.M. Pires0Maria P. Tomasino1Stéphane Simon2Rémy Buzier3Gilles Guibaud4C. Marisa R. Almeida5Ana P. Mucha6E2Lim – Eau et Environnement Limoges UR 24133, University of Limoges, Limoges, France; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal; FCUP – Faculty of Sciences, University of Porto, Porto, Portugal; Correspondence to: Laboratoire E2Lim (UR 24133), 123 avenue Albert Thomas, Limoges 87060, France.CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, PortugalE2Lim – Eau et Environnement Limoges UR 24133, University of Limoges, Limoges, FranceE2Lim – Eau et Environnement Limoges UR 24133, University of Limoges, Limoges, FranceE2Lim – Eau et Environnement Limoges UR 24133, University of Limoges, Limoges, FranceCIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal; FCUP – Faculty of Sciences, University of Porto, Porto, PortugalCIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal; FCUP – Faculty of Sciences, University of Porto, Porto, Portugal; Correspondence to: Laboratoire E2Lim (UR 24133), 123 avenue Albert Thomas, Limoges 87060, France.Trace elements (TEs) in anaerobic digestion (AD) are known to be essential for optimal biogas production, but inhibitive in excessive concentrations. However, the mechanisms of inhibition are not fully understood. The effects of the addition essential TEs (Co, Cu and Ni) and a non-essential TE (Cd) on the microbial community structure of AD were studied in lab-scale reactors, using total TE concentrations that ranged from 0 to 100 μM. Reactor performance was assessed by monitoring biogas production. The labile fraction of TEs (the most bioaccessible species) was determined by diffusive gradients in thin-films technique. Prokaryotic community composition was characterized through high throughput sequencing (HTS) targeting archaea and bacteria and qPCR to evaluate changes in methanogens and metal resistance genes. Only a minor fraction of added TEs was labile and it decreased over time, with Ni being the most labile. Although only a minor fraction of spiked concentration was labile, all TEs inhibited biogas production at the highest spiking concentration (100 μM), with higher inhibition observed for Cd and Ni. HTS and qPCR revealed changes, particularly in archaea, with reduced relative abundance at higher TE concentrations. Shifts in prokaryotic communities suggest alterations in AD metabolic pathways. High inhibition of biogas was linked to reduced diversity, dominance of the bacterial genus Klebsiella and changes in the ratio acetoclastic / hydrogenotrophic methanogens. This study addresses a research gap in understanding how TEs inhibit AD, and provides a strategy to improve TE dosing by monitoring the labile fraction of TEs to avoid overdosing.http://www.sciencedirect.com/science/article/pii/S0147651324015379Anaerobic digestionTrace elementsHigh throughput sequencingMicrobial communityLabile metalMetal resistance genes |
| spellingShingle | Rahul H.M. Pires Maria P. Tomasino Stéphane Simon Rémy Buzier Gilles Guibaud C. Marisa R. Almeida Ana P. Mucha Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure Ecotoxicology and Environmental Safety Anaerobic digestion Trace elements High throughput sequencing Microbial community Labile metal Metal resistance genes |
| title | Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure |
| title_full | Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure |
| title_fullStr | Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure |
| title_full_unstemmed | Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure |
| title_short | Impact of trace elements (total and labile fraction) on the anaerobic digestion activity and microbial community structure |
| title_sort | impact of trace elements total and labile fraction on the anaerobic digestion activity and microbial community structure |
| topic | Anaerobic digestion Trace elements High throughput sequencing Microbial community Labile metal Metal resistance genes |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015379 |
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