The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains
In this study, the acid resistance and desiccation tolerance of 20 strains of <i>Cronobacter malonaticus</i> were explored, and their genetic variances with respect to their survival in stressful conditions were identified by genomic analysis. The strains showed significant variances in...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Microbiology Research |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2036-7481/16/3/53 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849341989706792960 |
|---|---|
| author | Abdlrhman M. Alsonosi Khaled M. Ibrahim Bassam A. Elgamoudi Mahmoud B. Agena Stephen J. Forsythe |
| author_facet | Abdlrhman M. Alsonosi Khaled M. Ibrahim Bassam A. Elgamoudi Mahmoud B. Agena Stephen J. Forsythe |
| author_sort | Abdlrhman M. Alsonosi |
| collection | DOAJ |
| description | In this study, the acid resistance and desiccation tolerance of 20 strains of <i>Cronobacter malonaticus</i> were explored, and their genetic variances with respect to their survival in stressful conditions were identified by genomic analysis. The strains showed significant variances in acid tolerance when exposed to simulated gastric acid (pH 3.5) for 2 h. Strain 685 demonstrated less viability, suggesting greater susceptibility. Desiccation in infant formula also yielded sub-lethally injured cells, with variable strain recovery, highlighting strain 685 as the strain with the lowest recovery. Strains were determined to contain single nucleotide polymorphisms (SNPs) in the <i>ompR</i> and <i>rpoS</i> genes, suggesting loss-of-function mutations and potentially elucidating the stress sensitivity mechanism of strain 685. This study underscores the importance of genetic factors in <i>C. malonaticus</i> resilience and the necessity for developed detection methods for assessing food safety risk, especially in relation to powdered infant formulas. Our results provide important information on the pathogenic potential of <i>C. malonaticus</i> and help guide future research priorities to mitigate risks associated with foodborne pathogens. |
| format | Article |
| id | doaj-art-4c49440d69eb4b3f8fcc3eb208ccb2e6 |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj-art-4c49440d69eb4b3f8fcc3eb208ccb2e62025-08-20T03:43:31ZengMDPI AGMicrobiology Research2036-74812025-02-011635310.3390/microbiolres16030053The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> StrainsAbdlrhman M. Alsonosi0Khaled M. Ibrahim1Bassam A. Elgamoudi2Mahmoud B. Agena3Stephen J. Forsythe4Microbiology Department, Faculty of Medicine, Sebah University, Sebha P.O. Box 1000, LibyaMicrobiology Department, Libyan Biotechnology Research Center, Tripoli P.O. Box 30313, LibyaInstitute for Biomedicine and Glycomics, Griffith University, Southport 4215, AustraliaLibyan Medical Research Center, Zawia P.O. Box 20311, LibyaFoodmicrobe.com Ltd., Nottingham NG12 5GY, UKIn this study, the acid resistance and desiccation tolerance of 20 strains of <i>Cronobacter malonaticus</i> were explored, and their genetic variances with respect to their survival in stressful conditions were identified by genomic analysis. The strains showed significant variances in acid tolerance when exposed to simulated gastric acid (pH 3.5) for 2 h. Strain 685 demonstrated less viability, suggesting greater susceptibility. Desiccation in infant formula also yielded sub-lethally injured cells, with variable strain recovery, highlighting strain 685 as the strain with the lowest recovery. Strains were determined to contain single nucleotide polymorphisms (SNPs) in the <i>ompR</i> and <i>rpoS</i> genes, suggesting loss-of-function mutations and potentially elucidating the stress sensitivity mechanism of strain 685. This study underscores the importance of genetic factors in <i>C. malonaticus</i> resilience and the necessity for developed detection methods for assessing food safety risk, especially in relation to powdered infant formulas. Our results provide important information on the pathogenic potential of <i>C. malonaticus</i> and help guide future research priorities to mitigate risks associated with foodborne pathogens.https://www.mdpi.com/2036-7481/16/3/53<i>Cronobacter malonaticus</i>sub-lethally injured cellsdesiccationpowdered infant formula<i>OmpR</i><i>RpoS</i> |
| spellingShingle | Abdlrhman M. Alsonosi Khaled M. Ibrahim Bassam A. Elgamoudi Mahmoud B. Agena Stephen J. Forsythe The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains Microbiology Research <i>Cronobacter malonaticus</i> sub-lethally injured cells desiccation powdered infant formula <i>OmpR</i> <i>RpoS</i> |
| title | The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains |
| title_full | The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains |
| title_fullStr | The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains |
| title_full_unstemmed | The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains |
| title_short | The Potential Role of <i>rpoS</i> and <i>ompR</i> in the Acid Resistance and Desiccation Tolerance of <i>Cronobacter malonaticus</i> Strains |
| title_sort | potential role of i rpos i and i ompr i in the acid resistance and desiccation tolerance of i cronobacter malonaticus i strains |
| topic | <i>Cronobacter malonaticus</i> sub-lethally injured cells desiccation powdered infant formula <i>OmpR</i> <i>RpoS</i> |
| url | https://www.mdpi.com/2036-7481/16/3/53 |
| work_keys_str_mv | AT abdlrhmanmalsonosi thepotentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT khaledmibrahim thepotentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT bassamaelgamoudi thepotentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT mahmoudbagena thepotentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT stephenjforsythe thepotentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT abdlrhmanmalsonosi potentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT khaledmibrahim potentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT bassamaelgamoudi potentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT mahmoudbagena potentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains AT stephenjforsythe potentialroleofirposiandiompriintheacidresistanceanddesiccationtoleranceoficronobactermalonaticusistrains |