Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)

The aquaculture of olive flounder (Paralichthys olivaceus) is increasingly threatened by climate-induced elevated water temperatures, which negatively affect fish health and growth. This study explores the potential of dietary taurine supplementation to alleviate the effects of acute temperature str...

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Main Authors: Abayomi Oladimeji Ogun, Haham Kim, Sooa Yoon, Suhyun Lee, Hyuncheol Jeon, Deni Aulia, Junhyeok Hur, Seunghyung Lee
Format: Article
Language:English
Published: The Korean Society of Fisheries and Aquatic Science 2025-03-01
Series:Fisheries and Aquatic Sciences
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Online Access:http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2025.e15
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author Abayomi Oladimeji Ogun
Haham Kim
Sooa Yoon
Suhyun Lee
Hyuncheol Jeon
Deni Aulia
Junhyeok Hur
Seunghyung Lee
author_facet Abayomi Oladimeji Ogun
Haham Kim
Sooa Yoon
Suhyun Lee
Hyuncheol Jeon
Deni Aulia
Junhyeok Hur
Seunghyung Lee
author_sort Abayomi Oladimeji Ogun
collection DOAJ
description The aquaculture of olive flounder (Paralichthys olivaceus) is increasingly threatened by climate-induced elevated water temperatures, which negatively affect fish health and growth. This study explores the potential of dietary taurine supplementation to alleviate the effects of acute temperature stress on the physiological adaptability of olive flounder. In total, 420 juvenile fish, with an average initial weight of 12.97 ± 0.1 g (mean ± SEM), were randomly assigned to 21 tanks (20 fish per tank), forming seven triplicate groups. The fish were fed seven experimental diets: taurine 0.13% (Tau0.13), taurine 0.56% (Tau0.56), taurine 0.96% (Tau0.96), taurine 1.50% (Tau1.50), taurine 2.01% (Tau2.01), taurine 2.54% (Tau2.54), and taurine 2.87% (Tau2.87) at a rearing temperature of 19.5 ± 0.1°C. The dietary treatments were assigned randomly to the tanks, and a growth trial lasted for eight weeks. At the end of this period, one group of juveniles was exposed to lethal temperature stress (31°C) for 48 h, while another group were subjected to acute temperature stress (29°C) for 6 h. We assessed the effects of taurine across different biological levels. Growth performance remained relatively constant across all supplementation levels. However, there was a significant reduction in whole-body crude lipid content, indicating taurine’s role in fat metabolism. Cummulative survival rates under lethal temperature exposure showed no significant differences between the taurine-supplemented groups and the control (Tau0.13), suggesting that taurine alone may not mitigate the effects of extreme temperatures in juvenile olive flounder. Furthermore, taurine supplementation significantly altered the plasma free amino acid profile, reduced cortisol levels, and exhibited limited effectiveness in enhancing antioxidant enzyme activity and immune function. Additionally, no significant changes were observed in the expression levels of key stress-related genes in brain and liver tissues, including heat shock proteins (hsp60, hsp70, and hsp90) and the warm water acclimation-related gene (wap65), suggesting that taurine supplementation did not trigger a robust stress response at the molecular level. This research highlights taurine’s potential as a dietary intervention in aquaculture. However, further studies are needed to investigate its long-term effects under various environmental stressors.
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spelling doaj-art-4c46ceec1951447bba3d184d29b8a6122025-08-20T03:08:17ZengThe Korean Society of Fisheries and Aquatic ScienceFisheries and Aquatic Sciences2234-17572025-03-0128316318110.47853/FAS.2025.e15Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)Abayomi Oladimeji Ogun0Haham Kim1Sooa Yoon2Suhyun Lee3Hyuncheol Jeon4Deni Aulia5Junhyeok Hur6Seunghyung Lee7Major of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaMajor of Aquaculture and Applied Life Sciences, Division of Fisheries Life Sciences, Pukyong National University, Busan 48513, KoreaThe aquaculture of olive flounder (Paralichthys olivaceus) is increasingly threatened by climate-induced elevated water temperatures, which negatively affect fish health and growth. This study explores the potential of dietary taurine supplementation to alleviate the effects of acute temperature stress on the physiological adaptability of olive flounder. In total, 420 juvenile fish, with an average initial weight of 12.97 ± 0.1 g (mean ± SEM), were randomly assigned to 21 tanks (20 fish per tank), forming seven triplicate groups. The fish were fed seven experimental diets: taurine 0.13% (Tau0.13), taurine 0.56% (Tau0.56), taurine 0.96% (Tau0.96), taurine 1.50% (Tau1.50), taurine 2.01% (Tau2.01), taurine 2.54% (Tau2.54), and taurine 2.87% (Tau2.87) at a rearing temperature of 19.5 ± 0.1°C. The dietary treatments were assigned randomly to the tanks, and a growth trial lasted for eight weeks. At the end of this period, one group of juveniles was exposed to lethal temperature stress (31°C) for 48 h, while another group were subjected to acute temperature stress (29°C) for 6 h. We assessed the effects of taurine across different biological levels. Growth performance remained relatively constant across all supplementation levels. However, there was a significant reduction in whole-body crude lipid content, indicating taurine’s role in fat metabolism. Cummulative survival rates under lethal temperature exposure showed no significant differences between the taurine-supplemented groups and the control (Tau0.13), suggesting that taurine alone may not mitigate the effects of extreme temperatures in juvenile olive flounder. Furthermore, taurine supplementation significantly altered the plasma free amino acid profile, reduced cortisol levels, and exhibited limited effectiveness in enhancing antioxidant enzyme activity and immune function. Additionally, no significant changes were observed in the expression levels of key stress-related genes in brain and liver tissues, including heat shock proteins (hsp60, hsp70, and hsp90) and the warm water acclimation-related gene (wap65), suggesting that taurine supplementation did not trigger a robust stress response at the molecular level. This research highlights taurine’s potential as a dietary intervention in aquaculture. However, further studies are needed to investigate its long-term effects under various environmental stressors. http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2025.e15Climate changeTemperature toleranceFunctional additiveEnvironmental stressor
spellingShingle Abayomi Oladimeji Ogun
Haham Kim
Sooa Yoon
Suhyun Lee
Hyuncheol Jeon
Deni Aulia
Junhyeok Hur
Seunghyung Lee
Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)
Fisheries and Aquatic Sciences
Climate change
Temperature tolerance
Functional additive
Environmental stressor
title Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)
title_full Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)
title_fullStr Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)
title_full_unstemmed Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)
title_short Investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder (Paralichthys olivaceus)
title_sort investigating the potential of dietary taurine supplementation to mitigate temperature stress in juvenile olive flounder paralichthys olivaceus
topic Climate change
Temperature tolerance
Functional additive
Environmental stressor
url http://www.e-fas.org/archive/view_article?doi=10.47853/FAS.2025.e15
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