Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer

Abstract P2X7 receptor (P2X7R) plays a role in regulating tumor progression, but it is unclear whether P2X7R affects the pathological characteristics of patients with gastric cancer and the activity of gastric cancer cells. Therefore, this study preliminarily investigated the relationship between P2...

Full description

Saved in:
Bibliographic Details
Main Authors: Wen-jun Zhang, Xiong-wei Pi, Yi-guan Le, Teng-zheng Li
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-024-81515-7
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850048862387961856
author Wen-jun Zhang
Xiong-wei Pi
Yi-guan Le
Teng-zheng Li
author_facet Wen-jun Zhang
Xiong-wei Pi
Yi-guan Le
Teng-zheng Li
author_sort Wen-jun Zhang
collection DOAJ
description Abstract P2X7 receptor (P2X7R) plays a role in regulating tumor progression, but it is unclear whether P2X7R affects the pathological characteristics of patients with gastric cancer and the activity of gastric cancer cells. Therefore, this study preliminarily investigated the relationship between P2X7R and clinicopathological features of patients with gastric cancer, and further explored the effect of P2X7R on the proliferation, migration and invasion of gastric cancer cells through functional experiments. The results showed that P2X7R was highly expressed in gastric cancer tissues and gastric cancer cells. High expression of P2X7R was closely related to lymphatic metastasis, vascular invasion and Tumor-Node-Metastasis (TNM) stage in patients with gastric cancer. High expression of P2X7R predicted poor overall survival in patients. Moreover, the activation of P2X7R by ATP and its analogue BzATP increased the calcium current of gastric cancer cells, enhanced YF actin stress and cell viability, and promoted the proliferation, migration and invasion of gastric cancer cells. While P2X7R antagonists (A438079 and AZD9056) inhibited the proliferation, migration and invasion of gastric cancer cells induced by ATP. Therefore, the data obtained in this experiment suggest that P2X7R may be another potential molecular target for the prevention and treatment of gastric cancer.
format Article
id doaj-art-4c3ef25fc5014292bd3b7d65448fc786
institution DOAJ
issn 2045-2322
language English
publishDate 2024-12-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-4c3ef25fc5014292bd3b7d65448fc7862025-08-20T02:53:50ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-81515-7Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancerWen-jun Zhang0Xiong-wei Pi1Yi-guan Le2Teng-zheng Li3Department of Rehabilitation Medicine, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityGastrointestinal Surgery, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityGastrointestinal Surgery, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityDepartment of Gastroenterology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang UniversityAbstract P2X7 receptor (P2X7R) plays a role in regulating tumor progression, but it is unclear whether P2X7R affects the pathological characteristics of patients with gastric cancer and the activity of gastric cancer cells. Therefore, this study preliminarily investigated the relationship between P2X7R and clinicopathological features of patients with gastric cancer, and further explored the effect of P2X7R on the proliferation, migration and invasion of gastric cancer cells through functional experiments. The results showed that P2X7R was highly expressed in gastric cancer tissues and gastric cancer cells. High expression of P2X7R was closely related to lymphatic metastasis, vascular invasion and Tumor-Node-Metastasis (TNM) stage in patients with gastric cancer. High expression of P2X7R predicted poor overall survival in patients. Moreover, the activation of P2X7R by ATP and its analogue BzATP increased the calcium current of gastric cancer cells, enhanced YF actin stress and cell viability, and promoted the proliferation, migration and invasion of gastric cancer cells. While P2X7R antagonists (A438079 and AZD9056) inhibited the proliferation, migration and invasion of gastric cancer cells induced by ATP. Therefore, the data obtained in this experiment suggest that P2X7R may be another potential molecular target for the prevention and treatment of gastric cancer.https://doi.org/10.1038/s41598-024-81515-7Gastric cancer (GC)P2X7 receptor (P2X7R)Clinicopathological featuresProliferationMigration and invasion
spellingShingle Wen-jun Zhang
Xiong-wei Pi
Yi-guan Le
Teng-zheng Li
Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer
Scientific Reports
Gastric cancer (GC)
P2X7 receptor (P2X7R)
Clinicopathological features
Proliferation
Migration and invasion
title Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer
title_full Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer
title_fullStr Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer
title_full_unstemmed Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer
title_short Role of P2X7 receptor in the progression and clinicopathological characteristics of gastric cancer
title_sort role of p2x7 receptor in the progression and clinicopathological characteristics of gastric cancer
topic Gastric cancer (GC)
P2X7 receptor (P2X7R)
Clinicopathological features
Proliferation
Migration and invasion
url https://doi.org/10.1038/s41598-024-81515-7
work_keys_str_mv AT wenjunzhang roleofp2x7receptorintheprogressionandclinicopathologicalcharacteristicsofgastriccancer
AT xiongweipi roleofp2x7receptorintheprogressionandclinicopathologicalcharacteristicsofgastriccancer
AT yiguanle roleofp2x7receptorintheprogressionandclinicopathologicalcharacteristicsofgastriccancer
AT tengzhengli roleofp2x7receptorintheprogressionandclinicopathologicalcharacteristicsofgastriccancer