Prevalence of Extended Spectrum β-Lactamases-Producing Escherichia coli Isolated from Clinical Samples and Their Antibiotic Resistance Pattern
Introduction: Infections caused by ESBL-producing bacteria are a growing concern worldwide, especially in developing countries like Nigeria. Hence, this study aimed to isolate, screen, and identify ESBL-producing Escherichia coli from clinical samples and analyze their antibiotic resistance patte...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-03-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-509-en.html |
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| Summary: | Introduction: Infections caused by ESBL-producing bacteria are a growing concern
worldwide, especially in developing countries like Nigeria. Hence, this study aimed to
isolate, screen, and identify ESBL-producing Escherichia coli from clinical samples and
analyze their antibiotic resistance patterns. Methods: 200 clinical samples were
collected, consisting of 60 stool, 88 urine, and 52 wound pussamples. We used the pourplate
method on MaCconkey agar (MAC) for isolation. After suspected E. coli was
isolated, we sub-cultured it on eosin methylene blue (EMB) agar. To confirm E. coli
identification, we used cultural and biochemical assays. Disc and double disc diffusion
methods were employed to screen and confirm ESBL-producing E. coli. Antimicrobial
susceptibility testing was conducted by disc diffusion technique, and the results were
interpreted using CLSI standards. Results: A total of 47 E. coli isolates were obtained,
with 48.5% of the isolates originating from urine samples. These isolates were among
six different genera of bacteria. Among the E. coli isolates, 16 were confirmed to be
ESBL producers. The ESBL-producing E. coli demonstrated high resistance to
amoxicillin-clavulanic acid (87.5%), ampicillin (75.0%), and cefotaxime (50.0%).
Before plasmid curing, the bacteria demonstrated a high susceptibility to
chloramphenicol (81.3%) and amikacin (56.3%). However, varying antibiotic resistance
and susceptibility degrees were observed after plasmid curing. Conclusion: ESBLproducing
E. coli showed a high resistance level (34.0%) against most discs used.
However, chloramphenicol and amikacin showed promise as potential treatments for
ESBL-producing E. coli infections. In addition, it is recommended that clinical
laboratories should include routine ESBL detection methods for ongoing surveillance of
antibiotic-resistant isolates. This will serve as a helpful guide for empirically treating
bacterial infections. |
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| ISSN: | 2345-5349 2345-5330 |