Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo
Pyrazolone-based derivative metal complexes were reported to have cytotoxicity in some tumor cells. In this study, the antitumor effect of [Cu(PMPP-SAL)(EtOH)] (PMPP-SAL = N-(1-phenyl-3-methyl-4-propenylidene-5-pyrazolone)- salicylidene hydrazide anion) in murine melanoma B16 cells in vitro and in v...
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2020-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2020-0040 |
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| author | Abula Ayipairi Zhao Jing Xu Guancheng Li Yijie Sun Surong |
| author_facet | Abula Ayipairi Zhao Jing Xu Guancheng Li Yijie Sun Surong |
| author_sort | Abula Ayipairi |
| collection | DOAJ |
| description | Pyrazolone-based derivative metal complexes were reported to have cytotoxicity in some tumor cells. In this study, the antitumor effect of [Cu(PMPP-SAL)(EtOH)] (PMPP-SAL = N-(1-phenyl-3-methyl-4-propenylidene-5-pyrazolone)- salicylidene hydrazide anion) in murine melanoma B16 cells in vitro and in vivo was investigated. The results showed that [Cu(PMPP-SAL)(EtOH)] inhibited the survival of B16 cells in vitro, and the IC50 value was superior to cisplatin (DDP) (p < 0.001). B16 cell apoptosis was significantly higher in comparison to the control group (DMSO) (p < 0.01), and cell cycle arrest occurred at the G0/G1 phase. When challenged C57 BL/6J mice were treated with [Cu(PMPPSAL)(EtOH)], a smaller volume of B16 solid tumors were reported than the control group (p < 0.01), with lower positive expression indices of CD 34, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) (p < 0.01). Moreover, the tumor growth was suppressed in mice due to the induction of apoptosis, as detected by the TUNEL assay (p < 0.001). In summary, [Cu(PMPP-SAL)(EtOH)] effectively inhibited the growth of B16 cells in vitro and in vivo due to the induction of apoptosis and the inhibition of intra-tumoral angiogenesis, demonstrating its therapeutic potential in melanoma treatment. |
| format | Article |
| id | doaj-art-4c2e68ec3e234195ba7f6007c081be9b |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-4c2e68ec3e234195ba7f6007c081be9b2025-02-02T19:57:25ZengSciendoActa Pharmaceutica1846-95582020-12-0170456157510.2478/acph-2020-0040acph-2020-0040Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivoAbula Ayipairi0Zhao Jing1Xu Guancheng2Li Yijie3Sun Surong4Xinjiang Key Laboratory of Biological Resources and Genetic EngineeringCollege of Life Science and Technology Xinjiang University, Urumqi 830046 PR ChinaXinjiang Key Laboratory of Biological Resources and Genetic EngineeringCollege of Life Science and Technology Xinjiang University, Urumqi 830046 PR ChinaInstitute of Applied Chemistry, Xinjiang University, Urumqi 830046 PR ChinaXinjiang Key Laboratory of Biological Resources and Genetic EngineeringCollege of Life Science and Technology Xinjiang University, Urumqi 830046 PR ChinaXinjiang Key Laboratory of Biological Resources and Genetic EngineeringCollege of Life Science and Technology Xinjiang University, Urumqi 830046 PR ChinaPyrazolone-based derivative metal complexes were reported to have cytotoxicity in some tumor cells. In this study, the antitumor effect of [Cu(PMPP-SAL)(EtOH)] (PMPP-SAL = N-(1-phenyl-3-methyl-4-propenylidene-5-pyrazolone)- salicylidene hydrazide anion) in murine melanoma B16 cells in vitro and in vivo was investigated. The results showed that [Cu(PMPP-SAL)(EtOH)] inhibited the survival of B16 cells in vitro, and the IC50 value was superior to cisplatin (DDP) (p < 0.001). B16 cell apoptosis was significantly higher in comparison to the control group (DMSO) (p < 0.01), and cell cycle arrest occurred at the G0/G1 phase. When challenged C57 BL/6J mice were treated with [Cu(PMPPSAL)(EtOH)], a smaller volume of B16 solid tumors were reported than the control group (p < 0.01), with lower positive expression indices of CD 34, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) (p < 0.01). Moreover, the tumor growth was suppressed in mice due to the induction of apoptosis, as detected by the TUNEL assay (p < 0.001). In summary, [Cu(PMPP-SAL)(EtOH)] effectively inhibited the growth of B16 cells in vitro and in vivo due to the induction of apoptosis and the inhibition of intra-tumoral angiogenesis, demonstrating its therapeutic potential in melanoma treatment.https://doi.org/10.2478/acph-2020-0040malignant melanoma[cu(pmpp-sal)(etoh)]apoptosistumor microangiogenesis |
| spellingShingle | Abula Ayipairi Zhao Jing Xu Guancheng Li Yijie Sun Surong Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo Acta Pharmaceutica malignant melanoma [cu(pmpp-sal)(etoh)] apoptosis tumor microangiogenesis |
| title | Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo |
| title_full | Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo |
| title_fullStr | Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo |
| title_full_unstemmed | Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo |
| title_short | Antitumor effect of a pyrazolone-based-complex [Cu(PMPP-SAL)(EtOH)] against murine melanoma B16 cell in vitro and in vivo |
| title_sort | antitumor effect of a pyrazolone based complex cu pmpp sal etoh against murine melanoma b16 cell in vitro and in vivo |
| topic | malignant melanoma [cu(pmpp-sal)(etoh)] apoptosis tumor microangiogenesis |
| url | https://doi.org/10.2478/acph-2020-0040 |
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