Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration
Abstract Various mechanisms, including inflammation, oxidative stress, and apoptosis, are involved in the transition from acute kidney injury to chronic kidney disease (AKI-to-CKD). In this study, we aimed to determine the pathway linking acute injury and fibrosis under static magnetic fields (SMFs)...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-09077-w |
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| author | Seong Min Lee Saram Lee Kyu Hyeon Kim Daehan Kim Seong Joon Park Kyu Hong Kim Sunhwa Lee Eunjin Bae Kyung Don Yoo Jae Wook Lee Joong Yull Park Yon Su Kim Ran-hui Cha Seung Hee Yang |
| author_facet | Seong Min Lee Saram Lee Kyu Hyeon Kim Daehan Kim Seong Joon Park Kyu Hong Kim Sunhwa Lee Eunjin Bae Kyung Don Yoo Jae Wook Lee Joong Yull Park Yon Su Kim Ran-hui Cha Seung Hee Yang |
| author_sort | Seong Min Lee |
| collection | DOAJ |
| description | Abstract Various mechanisms, including inflammation, oxidative stress, and apoptosis, are involved in the transition from acute kidney injury to chronic kidney disease (AKI-to-CKD). In this study, we aimed to determine the pathway linking acute injury and fibrosis under static magnetic fields (SMFs). Human tubular epithelial cells (hTECs) were cultured on SMF platforms (119 mT; outward vs. inward direction) for 3 days, followed by treatment with adenine and p38 mitogen-activated protein kinase (MAPK) inhibitor to verify the role of MAPK pathway. In-vivo, mice were orally administered adenine (2mg/mouse/day) for 14 days to induce tubular injury, and p38 MAPK inhibitor (iP38, 10mg/kg) was injected intraperitoneally to evaluate its therapeutic effect. Inward SMF exposure significantly increased phospho-p38 (pp38) expression compared to outward SMFs. p38 MAPK inhibition reduced G1/S arrest and oxidative stress, apoptosis, and expression of fibrosis markers under inward SMFs. Additionally, iP38 treatment alleviated inflammation and fibrosis in adenine-induced tubular nephropathy (AITN). This study revealed that SMF-related AKI-to-CKD transition progresses with the direction of SMFs affecting the severity of injury, whereas p38 MAPK inhibition attenuates SMF-induced kidney injury and prevents fibrosis. |
| format | Article |
| id | doaj-art-4c2b3f2517e34ae48da7d725982237f2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4c2b3f2517e34ae48da7d725982237f22025-08-20T03:46:00ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-09077-wField direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alterationSeong Min Lee0Saram Lee1Kyu Hyeon Kim2Daehan Kim3Seong Joon Park4Kyu Hong Kim5Sunhwa Lee6Eunjin Bae7Kyung Don Yoo8Jae Wook Lee9Joong Yull Park10Yon Su Kim11Ran-hui Cha12Seung Hee Yang13Department of Biomedical Sciences, Seoul National UniversityDepartment of Transdisciplinary Medicine, Seoul National University HospitalDepartment of Biomedical Sciences, Seoul National UniversityDepartment of Medical Engineering, Graduate School, Chung-Ang UniversityDepartment of Biomedical Sciences, Seoul National UniversityDepartment of Biomedical Sciences, Seoul National UniversityDivision of Nephrology, Department of Internal Medicine, Kangwon National University HospitalInstitute of Medical Science, College of Medicine, Gyeongsang National UniversityDepartment of Internal Medicine, Ulsan University Hospital, University of Ulsan College of MedicineNephrology Clinic, National Cancer Center of KoreaDepartment of Medical Engineering, Graduate School, Chung-Ang UniversityDepartment of Internal Medicine, Seoul National University HospitalBiomedical Research Institute, Seoul National University HospitalDepartment of Kidney Research Institute, Seoul National University Medical Research CenterAbstract Various mechanisms, including inflammation, oxidative stress, and apoptosis, are involved in the transition from acute kidney injury to chronic kidney disease (AKI-to-CKD). In this study, we aimed to determine the pathway linking acute injury and fibrosis under static magnetic fields (SMFs). Human tubular epithelial cells (hTECs) were cultured on SMF platforms (119 mT; outward vs. inward direction) for 3 days, followed by treatment with adenine and p38 mitogen-activated protein kinase (MAPK) inhibitor to verify the role of MAPK pathway. In-vivo, mice were orally administered adenine (2mg/mouse/day) for 14 days to induce tubular injury, and p38 MAPK inhibitor (iP38, 10mg/kg) was injected intraperitoneally to evaluate its therapeutic effect. Inward SMF exposure significantly increased phospho-p38 (pp38) expression compared to outward SMFs. p38 MAPK inhibition reduced G1/S arrest and oxidative stress, apoptosis, and expression of fibrosis markers under inward SMFs. Additionally, iP38 treatment alleviated inflammation and fibrosis in adenine-induced tubular nephropathy (AITN). This study revealed that SMF-related AKI-to-CKD transition progresses with the direction of SMFs affecting the severity of injury, whereas p38 MAPK inhibition attenuates SMF-induced kidney injury and prevents fibrosis.https://doi.org/10.1038/s41598-025-09077-wAKI-to-CKDCell cycleMitogen-activated protein pathwayStatic magnetic field |
| spellingShingle | Seong Min Lee Saram Lee Kyu Hyeon Kim Daehan Kim Seong Joon Park Kyu Hong Kim Sunhwa Lee Eunjin Bae Kyung Don Yoo Jae Wook Lee Joong Yull Park Yon Su Kim Ran-hui Cha Seung Hee Yang Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration Scientific Reports AKI-to-CKD Cell cycle Mitogen-activated protein pathway Static magnetic field |
| title | Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration |
| title_full | Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration |
| title_fullStr | Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration |
| title_full_unstemmed | Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration |
| title_short | Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration |
| title_sort | field direction of static magnetic fields influences kidney fibrosis progression through mapk signaling and cell cycle alteration |
| topic | AKI-to-CKD Cell cycle Mitogen-activated protein pathway Static magnetic field |
| url | https://doi.org/10.1038/s41598-025-09077-w |
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