Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models.
Infectious bronchitis virus (IBV) is an important avian pathogen with a positive-sense single-stranded RNA genome. IBV is the causative agent of infectious bronchitis (IB), a primarily respiratory disease affecting chickens, with the ability to disseminate to other organ systems, such as the gastroi...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2025-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0319153 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849715788432277504 |
|---|---|
| author | Kelsey O'Dowd Safieh Vatandour Sadhiya S Ahamed Martine Boulianne Charles M Dozois Carl A Gagnon Neda Barjesteh Mohamed Faizal Abdul-Careem |
| author_facet | Kelsey O'Dowd Safieh Vatandour Sadhiya S Ahamed Martine Boulianne Charles M Dozois Carl A Gagnon Neda Barjesteh Mohamed Faizal Abdul-Careem |
| author_sort | Kelsey O'Dowd |
| collection | DOAJ |
| description | Infectious bronchitis virus (IBV) is an important avian pathogen with a positive-sense single-stranded RNA genome. IBV is the causative agent of infectious bronchitis (IB), a primarily respiratory disease affecting chickens, with the ability to disseminate to other organ systems, such as the gastrointestinal, renal, lymphoid, and reproductive systems. Tracheal epithelial cells are the primary target of IBV, and these cells play a vital role in the effective induction of the antiviral response and eventual clearance of IBV. The host immune system is regulated by a number of different molecular players, including micro-ribonucleic acids (microRNAs), which are small, conserved, non-coding RNA molecules that regulate gene expression of complementary messenger RNA (mRNA) sequences, resulting in gene silencing through translational repression or target degradation. The goal of this study was to characterize and compare the microRNA expression profiles in chicken tracheal epithelial cells (cTECs) in vitro and the trachea in vivo upon IBV Delmarva/1639 (DMV/1639) or IBV Massachusetts 41 (Mass41) infections. We hypothesized that IBV infection influences the expression of the host microRNA expression profiles. cTECs and young specific pathogen-free (SPF) chickens were infected with IBV DMV/1639 or IBV Mass41 and the microRNA expression at 3 and 18 hours post-infection (hpi) in the cTECs and at 4 and 11 days post-infection (dpi) in the trachea were determined using small RNA-sequencing (RNA-seq). We found that the profile of differentially expressed (DE) microRNAs is largely dependent on the IBV strain and time point of sample collection. Furthermore, we predicted the interaction between host microRNA and IBV viral RNA using microRNA-RNA interaction prediction platforms. We identified several candidate microRNAs suitable for future functional studies, such as gga-miR-155, gga-miR-1388a, gga-miR-7/7b and gga-miR-21-5p. Characterizing the interaction between IBV and the host cells at the level of microRNA regulation provides further insight into the regulatory mechanisms involved in viral infection and host defense in chickens following IBV infection. |
| format | Article |
| id | doaj-art-4c24f973c56d46d3aec79a5de611f5ce |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4c24f973c56d46d3aec79a5de611f5ce2025-08-20T03:13:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01203e031915310.1371/journal.pone.0319153Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models.Kelsey O'DowdSafieh VatandourSadhiya S AhamedMartine BoulianneCharles M DozoisCarl A GagnonNeda BarjestehMohamed Faizal Abdul-CareemInfectious bronchitis virus (IBV) is an important avian pathogen with a positive-sense single-stranded RNA genome. IBV is the causative agent of infectious bronchitis (IB), a primarily respiratory disease affecting chickens, with the ability to disseminate to other organ systems, such as the gastrointestinal, renal, lymphoid, and reproductive systems. Tracheal epithelial cells are the primary target of IBV, and these cells play a vital role in the effective induction of the antiviral response and eventual clearance of IBV. The host immune system is regulated by a number of different molecular players, including micro-ribonucleic acids (microRNAs), which are small, conserved, non-coding RNA molecules that regulate gene expression of complementary messenger RNA (mRNA) sequences, resulting in gene silencing through translational repression or target degradation. The goal of this study was to characterize and compare the microRNA expression profiles in chicken tracheal epithelial cells (cTECs) in vitro and the trachea in vivo upon IBV Delmarva/1639 (DMV/1639) or IBV Massachusetts 41 (Mass41) infections. We hypothesized that IBV infection influences the expression of the host microRNA expression profiles. cTECs and young specific pathogen-free (SPF) chickens were infected with IBV DMV/1639 or IBV Mass41 and the microRNA expression at 3 and 18 hours post-infection (hpi) in the cTECs and at 4 and 11 days post-infection (dpi) in the trachea were determined using small RNA-sequencing (RNA-seq). We found that the profile of differentially expressed (DE) microRNAs is largely dependent on the IBV strain and time point of sample collection. Furthermore, we predicted the interaction between host microRNA and IBV viral RNA using microRNA-RNA interaction prediction platforms. We identified several candidate microRNAs suitable for future functional studies, such as gga-miR-155, gga-miR-1388a, gga-miR-7/7b and gga-miR-21-5p. Characterizing the interaction between IBV and the host cells at the level of microRNA regulation provides further insight into the regulatory mechanisms involved in viral infection and host defense in chickens following IBV infection.https://doi.org/10.1371/journal.pone.0319153 |
| spellingShingle | Kelsey O'Dowd Safieh Vatandour Sadhiya S Ahamed Martine Boulianne Charles M Dozois Carl A Gagnon Neda Barjesteh Mohamed Faizal Abdul-Careem Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models. PLoS ONE |
| title | Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models. |
| title_full | Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models. |
| title_fullStr | Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models. |
| title_full_unstemmed | Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models. |
| title_short | Characterization of microRNA candidates at the primary site of infectious bronchitis virus infection: A comparative study of in vitro and in vivo avian models. |
| title_sort | characterization of microrna candidates at the primary site of infectious bronchitis virus infection a comparative study of in vitro and in vivo avian models |
| url | https://doi.org/10.1371/journal.pone.0319153 |
| work_keys_str_mv | AT kelseyodowd characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT safiehvatandour characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT sadhiyasahamed characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT martineboulianne characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT charlesmdozois characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT carlagagnon characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT nedabarjesteh characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels AT mohamedfaizalabdulcareem characterizationofmicrornacandidatesattheprimarysiteofinfectiousbronchitisvirusinfectionacomparativestudyofinvitroandinvivoavianmodels |