Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea
Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2 laser (10 W, 606 J/c...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/5982397 |
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| author | Xiang Liu Xiao-qing Qian Rui Ma Fang-Lu Chi Dong-Dong Ren |
| author_facet | Xiang Liu Xiao-qing Qian Rui Ma Fang-Lu Chi Dong-Dong Ren |
| author_sort | Xiang Liu |
| collection | DOAJ |
| description | Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2 laser (10 W, 606 J/cm2) to perform cochleostomies on guinea pig cochlea and to investigate the possible laser-induced damage mechanisms. The temperature changes in the round window membrane, auditory evoked brainstem response, and morphological of the hair cells were measured and recorded before and after laser application. All of the outcomes differed in comparison with the control group. A rise in temperature and subsequent increased hearing loss were observed in animals that underwent surgery with a 10 W CO2 laser. These findings correlated with increased injury to the cochlear ultrastructure and a higher positive expression of E-cadherin and β-catenin in the damaged organ of Corti. We assume that enhanced cell-cell adhesion and the activated β-catenin-related canonical Wnt-signalling pathway may play a role in the protection of the cochlea to prevent further damage. |
| format | Article |
| id | doaj-art-4c19a8b373e14700bf82036cd1bd272d |
| institution | DOAJ |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-4c19a8b373e14700bf82036cd1bd272d2025-08-20T03:20:13ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/59823975982397Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig CochleaXiang Liu0Xiao-qing Qian1Rui Ma2Fang-Lu Chi3Dong-Dong Ren4Department of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, ChinaDepartment of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, ChinaShanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, ChinaDepartment of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, ChinaDepartment of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, ChinaDifferent types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2 laser (10 W, 606 J/cm2) to perform cochleostomies on guinea pig cochlea and to investigate the possible laser-induced damage mechanisms. The temperature changes in the round window membrane, auditory evoked brainstem response, and morphological of the hair cells were measured and recorded before and after laser application. All of the outcomes differed in comparison with the control group. A rise in temperature and subsequent increased hearing loss were observed in animals that underwent surgery with a 10 W CO2 laser. These findings correlated with increased injury to the cochlear ultrastructure and a higher positive expression of E-cadherin and β-catenin in the damaged organ of Corti. We assume that enhanced cell-cell adhesion and the activated β-catenin-related canonical Wnt-signalling pathway may play a role in the protection of the cochlea to prevent further damage.http://dx.doi.org/10.1155/2016/5982397 |
| spellingShingle | Xiang Liu Xiao-qing Qian Rui Ma Fang-Lu Chi Dong-Dong Ren Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea Neural Plasticity |
| title | Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea |
| title_full | Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea |
| title_fullStr | Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea |
| title_full_unstemmed | Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea |
| title_short | Analysis of the Damage Mechanism Related to CO2 Laser Cochleostomy on Guinea Pig Cochlea |
| title_sort | analysis of the damage mechanism related to co2 laser cochleostomy on guinea pig cochlea |
| url | http://dx.doi.org/10.1155/2016/5982397 |
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