Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract
This study aims to evaluate potential toxic effect of Jatropha curcas leaves methanol extract on laboratory rats as well as determine its LD50. A total of 80 male Wistar rats were used as the experimental animals, 40 for LD50 determination and the other 40 for toxicity study. Based on the pretest th...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/798096 |
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| author | Osamuyimen O. Igbinosa Efosa F. Oviasogie Etinosa O. Igbinosa Otibhor Igene Isoken H. Igbinosa Omoruyi G. Idemudia |
| author_facet | Osamuyimen O. Igbinosa Efosa F. Oviasogie Etinosa O. Igbinosa Otibhor Igene Isoken H. Igbinosa Omoruyi G. Idemudia |
| author_sort | Osamuyimen O. Igbinosa |
| collection | DOAJ |
| description | This study aims to evaluate potential toxic effect of Jatropha curcas leaves methanol extract on laboratory rats as well as determine its LD50. A total of 80 male Wistar rats were used as the experimental animals, 40 for LD50 determination and the other 40 for toxicity study. Based on the pretest that was done in order to establish a range of toxicity, 4 dosages (86.00, 58.00, 46.00, and 34.0 kg/body weight) were chosen. The rats were randomly assigned into four groups with 10 rats in each group. Rats in groups 1, 2, 3, and 4 were given 0 mg/kg, 500 mg/kg, 1000 mg/kg, and 2000 mg/kg body weight of Jatropha curcas extract, respectively, by oral intubation for 21 days. Thereafter, clinical signs, change in body weight, toxicity symptoms, and biochemical parameters were obtained. The LD50 at 95% confidence limits for rats was 46.0 mg/kg body weight (44.95–52.69 mg/kg body mass). There was no clinical and biochemical signs of toxicity when the extract was administered at 500, 1000, and 2000 mg/kg body weight, respectively (P>0.05). Results obtained from this study suggest that liver, kidney, and haematological system of rats tolerated methanolic leave extract of Jatropha curcas at a certain concentration. |
| format | Article |
| id | doaj-art-4c12e67336cb4c6084f1f2dded75bc87 |
| institution | DOAJ |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4c12e67336cb4c6084f1f2dded75bc872025-08-20T03:20:13ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/798096798096Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf ExtractOsamuyimen O. Igbinosa0Efosa F. Oviasogie1Etinosa O. Igbinosa2Otibhor Igene3Isoken H. Igbinosa4Omoruyi G. Idemudia5Department of Medicine, Saint Peter’s University Hospital, New Brunswick, NJ, USADepartment of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, NigeriaDepartment of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, NigeriaCollege of Medicine, America University of Antigua, Antigua And BarbudaDepartment of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South AfricaDepartment of Chemistry, University of Fort Hare, Private Bag X1314, Alice 5700, South AfricaThis study aims to evaluate potential toxic effect of Jatropha curcas leaves methanol extract on laboratory rats as well as determine its LD50. A total of 80 male Wistar rats were used as the experimental animals, 40 for LD50 determination and the other 40 for toxicity study. Based on the pretest that was done in order to establish a range of toxicity, 4 dosages (86.00, 58.00, 46.00, and 34.0 kg/body weight) were chosen. The rats were randomly assigned into four groups with 10 rats in each group. Rats in groups 1, 2, 3, and 4 were given 0 mg/kg, 500 mg/kg, 1000 mg/kg, and 2000 mg/kg body weight of Jatropha curcas extract, respectively, by oral intubation for 21 days. Thereafter, clinical signs, change in body weight, toxicity symptoms, and biochemical parameters were obtained. The LD50 at 95% confidence limits for rats was 46.0 mg/kg body weight (44.95–52.69 mg/kg body mass). There was no clinical and biochemical signs of toxicity when the extract was administered at 500, 1000, and 2000 mg/kg body weight, respectively (P>0.05). Results obtained from this study suggest that liver, kidney, and haematological system of rats tolerated methanolic leave extract of Jatropha curcas at a certain concentration.http://dx.doi.org/10.1155/2013/798096 |
| spellingShingle | Osamuyimen O. Igbinosa Efosa F. Oviasogie Etinosa O. Igbinosa Otibhor Igene Isoken H. Igbinosa Omoruyi G. Idemudia Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract The Scientific World Journal |
| title | Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract |
| title_full | Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract |
| title_fullStr | Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract |
| title_full_unstemmed | Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract |
| title_short | Effects of Biochemical Alteration in Animal Model after Short-Term Exposure of Jatropha curcas (Linn) Leaf Extract |
| title_sort | effects of biochemical alteration in animal model after short term exposure of jatropha curcas linn leaf extract |
| url | http://dx.doi.org/10.1155/2013/798096 |
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