Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel
The scaffold-free tissue engineering using spheroids is pointed out as an approach for optimizing the delivery system of cartilage construct. In this study, we aimed to evaluate the micromolded nonadhesive hydrogel (MicroTissues®) for spheroid compaction (2-day culture) and spontaneous chondrogenesi...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7053465 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849406025909665792 |
|---|---|
| author | Mellannie P. Stuart Renata A. M. Matsui Matheus F. S. Santos Isis Côrtes Mayra S. Azevedo Karina R. Silva Anderson Beatrici Paulo Emílio C. Leite Priscila Falagan-Lotsch José M. Granjeiro Vladimir Mironov Leandra S. Baptista |
| author_facet | Mellannie P. Stuart Renata A. M. Matsui Matheus F. S. Santos Isis Côrtes Mayra S. Azevedo Karina R. Silva Anderson Beatrici Paulo Emílio C. Leite Priscila Falagan-Lotsch José M. Granjeiro Vladimir Mironov Leandra S. Baptista |
| author_sort | Mellannie P. Stuart |
| collection | DOAJ |
| description | The scaffold-free tissue engineering using spheroids is pointed out as an approach for optimizing the delivery system of cartilage construct. In this study, we aimed to evaluate the micromolded nonadhesive hydrogel (MicroTissues®) for spheroid compaction (2-day culture) and spontaneous chondrogenesis (21-day culture) using cartilage progenitors cells (CPCs) from human nasal septum without chondrogenic stimulus. CPC spheroids showed diameter stability (486 μm ± 65), high percentage of viable cells (88.1 ± 2.1), and low percentage of apoptotic cells (2.3%). After spheroid compaction, the synthesis of TGF-β1, TGF-β2, and TGF-β3 was significantly higher compared to monolayer (p<0.005). Biomechanical assay revealed that the maximum forces applied to spheroids after chondrogenesis were 2.6 times higher than for those cultured for 2 days. After spontaneous chondrogenesis, CPC spheroids were entirely positive for N-cadherin, collagen type II and type VI, and aggrecan and chondroitin sulfate. Comparing to monolayer, the expression of SOX5 and SOX6 genes analyzed by qPCR was significantly upregulated (p<0.01). Finally, we observed the capacity of CPC spheroids starting to fuse. To the best of our knowledge, this is the first time in the scientific literature that human CPC spheroids were formed by micromolded nonadhesive hydrogel, achieving a successful scaffold-free cartilage engineering without chondrogenic stimulus (low cost). |
| format | Article |
| id | doaj-art-4c0fe5efe55c4720b1ca9b1ee17e15b3 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4c0fe5efe55c4720b1ca9b1ee17e15b32025-08-20T03:36:31ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/70534657053465Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive HydrogelMellannie P. Stuart0Renata A. M. Matsui1Matheus F. S. Santos2Isis Côrtes3Mayra S. Azevedo4Karina R. Silva5Anderson Beatrici6Paulo Emílio C. Leite7Priscila Falagan-Lotsch8José M. Granjeiro9Vladimir Mironov10Leandra S. Baptista11Laboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilPrograma de Pós-graduação em Biotecnologia, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilLaboratório de Bioengenharia Tecidual, Instituto Nacional de Metrologia, Qualidade e Tecnologia (Inmetro), Duque de Caxias, RJ, BrazilThe scaffold-free tissue engineering using spheroids is pointed out as an approach for optimizing the delivery system of cartilage construct. In this study, we aimed to evaluate the micromolded nonadhesive hydrogel (MicroTissues®) for spheroid compaction (2-day culture) and spontaneous chondrogenesis (21-day culture) using cartilage progenitors cells (CPCs) from human nasal septum without chondrogenic stimulus. CPC spheroids showed diameter stability (486 μm ± 65), high percentage of viable cells (88.1 ± 2.1), and low percentage of apoptotic cells (2.3%). After spheroid compaction, the synthesis of TGF-β1, TGF-β2, and TGF-β3 was significantly higher compared to monolayer (p<0.005). Biomechanical assay revealed that the maximum forces applied to spheroids after chondrogenesis were 2.6 times higher than for those cultured for 2 days. After spontaneous chondrogenesis, CPC spheroids were entirely positive for N-cadherin, collagen type II and type VI, and aggrecan and chondroitin sulfate. Comparing to monolayer, the expression of SOX5 and SOX6 genes analyzed by qPCR was significantly upregulated (p<0.01). Finally, we observed the capacity of CPC spheroids starting to fuse. To the best of our knowledge, this is the first time in the scientific literature that human CPC spheroids were formed by micromolded nonadhesive hydrogel, achieving a successful scaffold-free cartilage engineering without chondrogenic stimulus (low cost).http://dx.doi.org/10.1155/2017/7053465 |
| spellingShingle | Mellannie P. Stuart Renata A. M. Matsui Matheus F. S. Santos Isis Côrtes Mayra S. Azevedo Karina R. Silva Anderson Beatrici Paulo Emílio C. Leite Priscila Falagan-Lotsch José M. Granjeiro Vladimir Mironov Leandra S. Baptista Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel Stem Cells International |
| title | Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel |
| title_full | Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel |
| title_fullStr | Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel |
| title_full_unstemmed | Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel |
| title_short | Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel |
| title_sort | successful low cost scaffold free cartilage tissue engineering using human cartilage progenitor cell spheroids formed by micromolded nonadhesive hydrogel |
| url | http://dx.doi.org/10.1155/2017/7053465 |
| work_keys_str_mv | AT mellanniepstuart successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT renataammatsui successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT matheusfssantos successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT isiscortes successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT mayrasazevedo successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT karinarsilva successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT andersonbeatrici successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT pauloemiliocleite successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT priscilafalaganlotsch successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT josemgranjeiro successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT vladimirmironov successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel AT leandrasbaptista successfullowcostscaffoldfreecartilagetissueengineeringusinghumancartilageprogenitorcellspheroidsformedbymicromoldednonadhesivehydrogel |