Recent advances in biosensors for analysis of DNA/RNA methylation
DNA and RNA methylation are essential epigenetic modifications that play a pivotal role in various physiological processes by modulating gene expression at the post-transcriptional level. Abnormal DNA/RNA methylation is closely linked to a range of human diseases, including cancer and neurological d...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180424000722 |
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| author | Zhensheng Ma Yue Hu Lina Wang Mimi Li Chenghong Li Lulu Li Hui Huang Lichao Fang Xiaolong Wang Huamin Liu Junsong Zheng |
| author_facet | Zhensheng Ma Yue Hu Lina Wang Mimi Li Chenghong Li Lulu Li Hui Huang Lichao Fang Xiaolong Wang Huamin Liu Junsong Zheng |
| author_sort | Zhensheng Ma |
| collection | DOAJ |
| description | DNA and RNA methylation are essential epigenetic modifications that play a pivotal role in various physiological processes by modulating gene expression at the post-transcriptional level. Abnormal DNA/RNA methylation is closely linked to a range of human diseases, including cancer and neurological disorders. Therefore, the accurate and sensitive detection of DNA/RNA methylation is of great importance in both epigenetic biology research and disease diagnosis. Technological advancements have led to the comprehensive development of methylation detection methods. Among these, electrochemical techniques have garnered significant attention owing to their simplicity and effectiveness in detection. This review provides an overview of methylation detection technologies based on electrochemical principles developed over the past five years, including electrochemical, photoelectrochemical, electrochemiluminescence, and fluorescence methodologies. Additionally, it discusses the advantages and disadvantages of these approaches, challenges and advancements in methylation detection. |
| format | Article |
| id | doaj-art-4c040dbfc0f14024b32fff39671f9431 |
| institution | OA Journals |
| issn | 2214-1804 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-4c040dbfc0f14024b32fff39671f94312025-08-20T02:20:48ZengElsevierSensing and Bio-Sensing Research2214-18042024-12-014610069010.1016/j.sbsr.2024.100690Recent advances in biosensors for analysis of DNA/RNA methylationZhensheng Ma0Yue Hu1Lina Wang2Mimi Li3Chenghong Li4Lulu Li5Hui Huang6Lichao Fang7Xiaolong Wang8Huamin Liu9Junsong Zheng10Department of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaEmergency Department, 2nd Affiliated Hospital of Chongqing Medical University, 400010 Chongqing, ChinaDepartment of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaEmergency Department, 2nd Affiliated Hospital of Chongqing Medical University, 400010 Chongqing, China; Department of Anesthesia, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaDepartment of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaDepartment of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaDepartment of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaDepartment of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, ChinaEmergency Department, 2nd Affiliated Hospital of Chongqing Medical University, 400010 Chongqing, China; Corresponding authors.Department of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, China; Corresponding authors.Department of Clinical and Military Laboratory Medicine, College of Pharmacy and Laboratory Medicine, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, China; Department of Clinical Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, China; Corresponding author at: Department of Clinical and Military Laboratory Medicine, College of Medical Laboratory Science, Army Medical University, No. 30 Gaotanyan Street, Shapingba District, 400038 Chongqing, China.DNA and RNA methylation are essential epigenetic modifications that play a pivotal role in various physiological processes by modulating gene expression at the post-transcriptional level. Abnormal DNA/RNA methylation is closely linked to a range of human diseases, including cancer and neurological disorders. Therefore, the accurate and sensitive detection of DNA/RNA methylation is of great importance in both epigenetic biology research and disease diagnosis. Technological advancements have led to the comprehensive development of methylation detection methods. Among these, electrochemical techniques have garnered significant attention owing to their simplicity and effectiveness in detection. This review provides an overview of methylation detection technologies based on electrochemical principles developed over the past five years, including electrochemical, photoelectrochemical, electrochemiluminescence, and fluorescence methodologies. Additionally, it discusses the advantages and disadvantages of these approaches, challenges and advancements in methylation detection.http://www.sciencedirect.com/science/article/pii/S2214180424000722DNA methylationRNA methylationElectrochemistryBiosensorDemethylation |
| spellingShingle | Zhensheng Ma Yue Hu Lina Wang Mimi Li Chenghong Li Lulu Li Hui Huang Lichao Fang Xiaolong Wang Huamin Liu Junsong Zheng Recent advances in biosensors for analysis of DNA/RNA methylation Sensing and Bio-Sensing Research DNA methylation RNA methylation Electrochemistry Biosensor Demethylation |
| title | Recent advances in biosensors for analysis of DNA/RNA methylation |
| title_full | Recent advances in biosensors for analysis of DNA/RNA methylation |
| title_fullStr | Recent advances in biosensors for analysis of DNA/RNA methylation |
| title_full_unstemmed | Recent advances in biosensors for analysis of DNA/RNA methylation |
| title_short | Recent advances in biosensors for analysis of DNA/RNA methylation |
| title_sort | recent advances in biosensors for analysis of dna rna methylation |
| topic | DNA methylation RNA methylation Electrochemistry Biosensor Demethylation |
| url | http://www.sciencedirect.com/science/article/pii/S2214180424000722 |
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