Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae)
Biopesticides from botanicals are nowadays actively encouraged in the mosquito control program because of their low mammalian toxicity, biodegradability, and target specificity. However, leaf methanolic extracts and essential oils of Callistemon rigidus and Eucalyptus camaldulensis were evaluated in...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Zoology |
| Online Access: | http://dx.doi.org/10.1155/2020/4952041 |
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| author | Lame Younoussa Francine Kenmoe Mallam Kary Oumarou Aurore Christelle Sengue Batti Joseph Lebel Tamesse Elias Nchiwan Nukenine |
| author_facet | Lame Younoussa Francine Kenmoe Mallam Kary Oumarou Aurore Christelle Sengue Batti Joseph Lebel Tamesse Elias Nchiwan Nukenine |
| author_sort | Lame Younoussa |
| collection | DOAJ |
| description | Biopesticides from botanicals are nowadays actively encouraged in the mosquito control program because of their low mammalian toxicity, biodegradability, and target specificity. However, leaf methanolic extracts and essential oils of Callistemon rigidus and Eucalyptus camaldulensis were evaluated individually and in combination on third- and fourth-instar larvae of Anopheles gambiae. The extracts were tested individually and in combination at doses of 125, 250, 500, and 1000 ppm while essential oils were applied at 25, 50, 100, and 200 according to the standard protocol of WHO. The commercial insecticide Bi-one tested at the recommended dose of 1000 ppm was used as positive control while the solution of tap water containing 0.5 ml of methanol was used as negative control. The mortality of the larvae was recorded after 24 h postexposure. In the results, 100% mortality of the larvae was recorded with the extracts of C. rigidus and the combinations E50% : C50% and E25% : C75% of the plants as well as positive control (1000 ppm). Similarly, essential oils of the two plants and their combinations caused 100% mortality of the larvae. Among the various combinations of the extracts and essential oils, only the combination E75% : C25% of essential oils presented a synergistic effect. Therefore, the essential oil combination (E75% : C25%) of the plants E. camaldulensis and C. rigidus is recommended in order to promote the its use in the form of natural biocide in the implementation of effective insect controls against the mosquito larvae, vector of malaria. |
| format | Article |
| id | doaj-art-4c038955e794458f9687ecf52ff243e4 |
| institution | DOAJ |
| issn | 1687-8477 1687-8485 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Zoology |
| spelling | doaj-art-4c038955e794458f9687ecf52ff243e42025-08-20T03:20:13ZengWileyInternational Journal of Zoology1687-84771687-84852020-01-01202010.1155/2020/49520414952041Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae)Lame Younoussa0Francine Kenmoe1Mallam Kary Oumarou2Aurore Christelle Sengue Batti3Joseph Lebel Tamesse4Elias Nchiwan Nukenine5Institute of Medical Research and Medicinal Plants Studies, P.O. Box 13033, Yaoundé, CameroonDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, CameroonDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, CameroonDepartment of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundere, CameroonDepartment of Biological Sciences, Higher Teacher’s Training College, University of Yaounde I, P.O. Box 47, Yaounde, CameroonDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaounde 1, P.O. Box 812, Yaounde, CameroonBiopesticides from botanicals are nowadays actively encouraged in the mosquito control program because of their low mammalian toxicity, biodegradability, and target specificity. However, leaf methanolic extracts and essential oils of Callistemon rigidus and Eucalyptus camaldulensis were evaluated individually and in combination on third- and fourth-instar larvae of Anopheles gambiae. The extracts were tested individually and in combination at doses of 125, 250, 500, and 1000 ppm while essential oils were applied at 25, 50, 100, and 200 according to the standard protocol of WHO. The commercial insecticide Bi-one tested at the recommended dose of 1000 ppm was used as positive control while the solution of tap water containing 0.5 ml of methanol was used as negative control. The mortality of the larvae was recorded after 24 h postexposure. In the results, 100% mortality of the larvae was recorded with the extracts of C. rigidus and the combinations E50% : C50% and E25% : C75% of the plants as well as positive control (1000 ppm). Similarly, essential oils of the two plants and their combinations caused 100% mortality of the larvae. Among the various combinations of the extracts and essential oils, only the combination E75% : C25% of essential oils presented a synergistic effect. Therefore, the essential oil combination (E75% : C25%) of the plants E. camaldulensis and C. rigidus is recommended in order to promote the its use in the form of natural biocide in the implementation of effective insect controls against the mosquito larvae, vector of malaria.http://dx.doi.org/10.1155/2020/4952041 |
| spellingShingle | Lame Younoussa Francine Kenmoe Mallam Kary Oumarou Aurore Christelle Sengue Batti Joseph Lebel Tamesse Elias Nchiwan Nukenine Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae) International Journal of Zoology |
| title | Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae) |
| title_full | Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae) |
| title_fullStr | Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae) |
| title_full_unstemmed | Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae) |
| title_short | Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae) |
| title_sort | combined effect of methanol extracts and essential oils of callistemon rigidus myrtaceae and eucalyptus camaldulensis myrtaceae against anopheles gambiae giles larvae diptera culicidae |
| url | http://dx.doi.org/10.1155/2020/4952041 |
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