Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients
Cartilage-derived migratory cells show great potential for autologous use in cartilage repair surgery. However, their collection through arthroscopic biopsy has not been previously reported in individuals without osteoarthritis. This study aimed to characterize migratory cartilage cells isolated fro...
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MDPI AG
2025-06-01
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| author | Pedro Nogueira Giglio Débora Levy Phelipe Oliveira Favaron Lucas da Ponte Melo Cadiele Oliana Reichert Fábio Alessandro de Freitas Juliana Sampaio Silva Walcy Paganelli Rosolia Teodoro Sérgio Paulo Bydlowski Marco Kawamura Demange |
| author_facet | Pedro Nogueira Giglio Débora Levy Phelipe Oliveira Favaron Lucas da Ponte Melo Cadiele Oliana Reichert Fábio Alessandro de Freitas Juliana Sampaio Silva Walcy Paganelli Rosolia Teodoro Sérgio Paulo Bydlowski Marco Kawamura Demange |
| author_sort | Pedro Nogueira Giglio |
| collection | DOAJ |
| description | Cartilage-derived migratory cells show great potential for autologous use in cartilage repair surgery. However, their collection through arthroscopic biopsy has not been previously reported in individuals without osteoarthritis. This study aimed to characterize migratory cartilage cells isolated from arthroscopic biopsies of volunteers without osteoarthritis and compare them with cells obtained by enzymatic digestion. Cell cultures were successfully established using both methods—enzymatic digestion and cell migration—from cartilage explants, with no significant differences observed in stem cell markers or plasticity between the cell lines. Cells derived from both procedures exhibited characteristics of mesenchymal stem cell, including fibroblast-like morphology, expression of CD29, CD90, and CD105 markers, absence of hematopoietic and endothelial cell markers, and the ability to differentiate into adipocytes, chondrocytes, and osteoblasts under appropriate conditions. Cells obtained by migration showed lower expression of collagen I and II, along with reduce collagen II/collagen I ratio, both positively associated with chondral matrix production, as well as lower RUNX2 expression. However, no differences were found in the levels of SOX9, essential for chondrogenic differentiation, or in the expression of <i>perlecan</i> gene. <i>Syndecan-1</i> expression was lower in cells obtained by migration. In conclusion, this study demonstrates that cartilage-derived migratory cells can be successfully obtained from arthroscopic biopsies of individuals without osteoarthritis, presenting comparable dedifferentiation and plasticity profiles. Furthermore, these cells express essential chondrogenic markers and proteins. Although further in vivo studies are needed to determine their effective regenerative potential, cartilage-derived migratory cells represent a promising avenue for cartilage repair strategies. |
| format | Article |
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| institution | OA Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-4c00a5846caa46b29f4364a672d91d5a2025-08-20T02:23:01ZengMDPI AGCells2073-44092025-06-01141183010.3390/cells14110830Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic PatientsPedro Nogueira Giglio0Débora Levy1Phelipe Oliveira Favaron2Lucas da Ponte Melo3Cadiele Oliana Reichert4Fábio Alessandro de Freitas5Juliana Sampaio Silva6Walcy Paganelli Rosolia Teodoro7Sérgio Paulo Bydlowski8Marco Kawamura Demange9Instituto de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilLipids, Oxidation and Cell Biology Team, Laboratory of Immunology (LIM19), Heart Institute (InCor), Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilCenter of Biological Sciences, Departament of General Biology, Universidade Estadual de Londrina, Paraná 86057-970, BrazilInstituto de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilLipids, Oxidation and Cell Biology Team, Laboratory of Immunology (LIM19), Heart Institute (InCor), Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilLipids, Oxidation and Cell Biology Team, Laboratory of Immunology (LIM19), Heart Institute (InCor), Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilLipids, Oxidation and Cell Biology Team, Laboratory of Immunology (LIM19), Heart Institute (InCor), Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilLaboratory of Extracelular Matrix, Reumatology Discipline and Bioterium of the Department of Clinical Medicine, Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 01246-903, BrazilLipids, Oxidation and Cell Biology Team, Laboratory of Immunology (LIM19), Heart Institute (InCor), Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilInstituto de Ortopedia e Traumatologia, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo-HCFMUSP, Sao Paulo 05403-010, BrazilCartilage-derived migratory cells show great potential for autologous use in cartilage repair surgery. However, their collection through arthroscopic biopsy has not been previously reported in individuals without osteoarthritis. This study aimed to characterize migratory cartilage cells isolated from arthroscopic biopsies of volunteers without osteoarthritis and compare them with cells obtained by enzymatic digestion. Cell cultures were successfully established using both methods—enzymatic digestion and cell migration—from cartilage explants, with no significant differences observed in stem cell markers or plasticity between the cell lines. Cells derived from both procedures exhibited characteristics of mesenchymal stem cell, including fibroblast-like morphology, expression of CD29, CD90, and CD105 markers, absence of hematopoietic and endothelial cell markers, and the ability to differentiate into adipocytes, chondrocytes, and osteoblasts under appropriate conditions. Cells obtained by migration showed lower expression of collagen I and II, along with reduce collagen II/collagen I ratio, both positively associated with chondral matrix production, as well as lower RUNX2 expression. However, no differences were found in the levels of SOX9, essential for chondrogenic differentiation, or in the expression of <i>perlecan</i> gene. <i>Syndecan-1</i> expression was lower in cells obtained by migration. In conclusion, this study demonstrates that cartilage-derived migratory cells can be successfully obtained from arthroscopic biopsies of individuals without osteoarthritis, presenting comparable dedifferentiation and plasticity profiles. Furthermore, these cells express essential chondrogenic markers and proteins. Although further in vivo studies are needed to determine their effective regenerative potential, cartilage-derived migratory cells represent a promising avenue for cartilage repair strategies.https://www.mdpi.com/2073-4409/14/11/830cartilage-derived cellscartilage repairmesenchymal stem cellscartilage explantsenzymatic cartilage digestion |
| spellingShingle | Pedro Nogueira Giglio Débora Levy Phelipe Oliveira Favaron Lucas da Ponte Melo Cadiele Oliana Reichert Fábio Alessandro de Freitas Juliana Sampaio Silva Walcy Paganelli Rosolia Teodoro Sérgio Paulo Bydlowski Marco Kawamura Demange Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients Cells cartilage-derived cells cartilage repair mesenchymal stem cells cartilage explants enzymatic cartilage digestion |
| title | Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients |
| title_full | Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients |
| title_fullStr | Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients |
| title_full_unstemmed | Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients |
| title_short | Isolation and Characterization of Articular Cartilage-Derived Cells Obtained by Arthroscopic Cartilage Biopsy from Non-Osteoarthritic Patients |
| title_sort | isolation and characterization of articular cartilage derived cells obtained by arthroscopic cartilage biopsy from non osteoarthritic patients |
| topic | cartilage-derived cells cartilage repair mesenchymal stem cells cartilage explants enzymatic cartilage digestion |
| url | https://www.mdpi.com/2073-4409/14/11/830 |
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