Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes
Abstract Soil salinization poses a significant challenge for rice farming, affecting approximately 20% of irrigated land worldwide. It leads to osmotic stress, ionic toxicity, and oxidative damage, severely hindering growth and yield. This study investigates the potential of lignin-containing cellul...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-98906-z |
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| author | Abdelghany S. Shaban Ragab Abouzeid Qinglin Wu Prasanta K. Subudhi |
| author_facet | Abdelghany S. Shaban Ragab Abouzeid Qinglin Wu Prasanta K. Subudhi |
| author_sort | Abdelghany S. Shaban |
| collection | DOAJ |
| description | Abstract Soil salinization poses a significant challenge for rice farming, affecting approximately 20% of irrigated land worldwide. It leads to osmotic stress, ionic toxicity, and oxidative damage, severely hindering growth and yield. This study investigates the potential of lignin-containing cellulose nanofiber (LCNF)-selenium nanoparticle (SeNPs) hybrids to enhance salt tolerance in rice, focusing on two rice genotypes with contrasting responses to salt stress. LCNF-SeNP hybrids were synthesized using a microwave-assisted green synthesis method and characterized through FTIR, X-ray diffraction, SEM, TEM, and TGA. The effects of LCNF/SeNPs on seed germination, physiological responses, and gene expression were evaluated under varying levels of NaCl-induced salt stress. Results indicated that LCNF/SeNPs significantly enhanced the salt tolerance of the salt-sensitive genotype IR29, as evidenced by increased germination rates, reduced salt injury scores, and higher chlorophyll content. For the salt-tolerant genotype TCCP, LCNF/SeNPs improved shoot lengths and maintained elevated chlorophyll levels under salt stress. Furthermore, LCNF/SeNPs improved ion homeostasis in both genotypes by reducing the Na+/K+ ratio, which is crucial for maintaining cellular function under salt stress. Gene expression analysis revealed upregulation of key salt stress-responsive genes, suggesting enhanced stress tolerance due to the application of LCNF/SeNPs in both genotypes. This study underscores the potential of LCNF/SeNPs as a sustainable strategy for improving crop performance in saline environments. |
| format | Article |
| id | doaj-art-4bfece88fee749cbb6197236815910da |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-4bfece88fee749cbb6197236815910da2025-08-20T03:14:09ZengNature PortfolioScientific Reports2045-23222025-04-0115111510.1038/s41598-025-98906-zLignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypesAbdelghany S. Shaban0Ragab Abouzeid1Qinglin Wu2Prasanta K. Subudhi3School of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural CenterSchool of Renewable Natural Resources, Louisiana State University Agricultural CenterSchool of Renewable Natural Resources, Louisiana State University Agricultural CenterSchool of Plant, Environmental, and Soil Sciences, Louisiana State University Agricultural CenterAbstract Soil salinization poses a significant challenge for rice farming, affecting approximately 20% of irrigated land worldwide. It leads to osmotic stress, ionic toxicity, and oxidative damage, severely hindering growth and yield. This study investigates the potential of lignin-containing cellulose nanofiber (LCNF)-selenium nanoparticle (SeNPs) hybrids to enhance salt tolerance in rice, focusing on two rice genotypes with contrasting responses to salt stress. LCNF-SeNP hybrids were synthesized using a microwave-assisted green synthesis method and characterized through FTIR, X-ray diffraction, SEM, TEM, and TGA. The effects of LCNF/SeNPs on seed germination, physiological responses, and gene expression were evaluated under varying levels of NaCl-induced salt stress. Results indicated that LCNF/SeNPs significantly enhanced the salt tolerance of the salt-sensitive genotype IR29, as evidenced by increased germination rates, reduced salt injury scores, and higher chlorophyll content. For the salt-tolerant genotype TCCP, LCNF/SeNPs improved shoot lengths and maintained elevated chlorophyll levels under salt stress. Furthermore, LCNF/SeNPs improved ion homeostasis in both genotypes by reducing the Na+/K+ ratio, which is crucial for maintaining cellular function under salt stress. Gene expression analysis revealed upregulation of key salt stress-responsive genes, suggesting enhanced stress tolerance due to the application of LCNF/SeNPs in both genotypes. This study underscores the potential of LCNF/SeNPs as a sustainable strategy for improving crop performance in saline environments.https://doi.org/10.1038/s41598-025-98906-zSeleniumCelluloseLigninNanoparticlesSalt stressRice |
| spellingShingle | Abdelghany S. Shaban Ragab Abouzeid Qinglin Wu Prasanta K. Subudhi Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes Scientific Reports Selenium Cellulose Lignin Nanoparticles Salt stress Rice |
| title | Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes |
| title_full | Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes |
| title_fullStr | Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes |
| title_full_unstemmed | Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes |
| title_short | Lignin-containing cellulose nanofiber-selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes |
| title_sort | lignin containing cellulose nanofiber selenium nanoparticle hybrid enhances tolerance to salt stress in rice genotypes |
| topic | Selenium Cellulose Lignin Nanoparticles Salt stress Rice |
| url | https://doi.org/10.1038/s41598-025-98906-z |
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