Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells
Therapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2012-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/63 |
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| _version_ | 1849323385903906816 |
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| author | Yurista Gilang Adam Hermawan Aditya Fitriasari Riris Istighfari Jenie |
| author_facet | Yurista Gilang Adam Hermawan Aditya Fitriasari Riris Istighfari Jenie |
| author_sort | Yurista Gilang |
| collection | DOAJ |
| description | Therapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation. Either single treatment of hesperidin 200µM or 5-FU 1500 µM did not trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms.
Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosis |
| format | Article |
| id | doaj-art-4bf8aae3b63d43acb260f4bd36b6909e |
| institution | Kabale University |
| issn | 2088-0197 2355-8989 |
| language | English |
| publishDate | 2012-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj-art-4bf8aae3b63d43acb260f4bd36b6909e2025-08-20T03:49:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892012-06-013240440910.14499/indonesianjcanchemoprev3iss2pp404-40964Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr CellsYurista Gilang0Adam Hermawan1Aditya Fitriasari2Riris Istighfari Jenie3Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah MadaCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah MadaTherapy of colon cancer by using 5-FU often causes problems of resistance. This encourages the development of co-chemotherapy agent. One of the compounds that could potentially be used as a co-chemotherapy agent is hesperidin. This study was conducted to determine the cytotoxic effects of hesperidin, 5-FU and the combination of them, as well as apoptosis induction in colon cancer cells WiDr. Cytotoxic effect of hesperidin, 5-FU, and its combination were observed using MTT assay. Observation of apoptosis was done by double staining method using ethidium bromide-acridin orange. Until 48 hours incubation, hesperidin showed no cytotoxic effects. Cytotoxic effects of 5-FU was observed after 48 hours with the IC50 value of 422 µM. However, hesperidin improved the cytotoxic effects of 5-FU at 48 hour incubation. Either single treatment of hesperidin 200µM or 5-FU 1500 µM did not trigger apoptosis, but combination of them led to the emergence of signs of apoptosis. Based on this study,it can be concluded thathesperidin is potential to be developed as a co-chemotherapy agent of 5-FU on colon cancer but still need further study on its molecular mechanisms. Keywords : hesperidin, 5-fluorouracil, WiDr cells, cytotoxic, apoptosishttps://ijcc.chemoprev.org/index.php/ijcc/article/view/63 |
| spellingShingle | Yurista Gilang Adam Hermawan Aditya Fitriasari Riris Istighfari Jenie Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells |
| title_full | Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells |
| title_fullStr | Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells |
| title_full_unstemmed | Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells |
| title_short | Hesperidin Increases Cytotoxic Effect of 5-Fluorouracil on WiDr Cells |
| title_sort | hesperidin increases cytotoxic effect of 5 fluorouracil on widr cells |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/63 |
| work_keys_str_mv | AT yuristagilang hesperidinincreasescytotoxiceffectof5fluorouracilonwidrcells AT adamhermawan hesperidinincreasescytotoxiceffectof5fluorouracilonwidrcells AT adityafitriasari hesperidinincreasescytotoxiceffectof5fluorouracilonwidrcells AT ririsistighfarijenie hesperidinincreasescytotoxiceffectof5fluorouracilonwidrcells |