Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones
An important problem of our time is the resistance of bacteria to antimicrobial drugs. Surface water bodies accumulate all kinds of antibiotic-resistant bacteria found in the catchment area.The aim. To compare the antibiotic resistance of enterobacteria isolated from freshwater ecosystems of the Mur...
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Scientific Сentre for Family Health and Human Reproduction Problems
2022-07-01
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| Series: | Acta Biomedica Scientifica |
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| Online Access: | https://www.actabiomedica.ru/jour/article/view/3561 |
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| author | E. A. Zatsarinnaya N. V. Kolupaeva L. V. Kolupaeva |
| author_facet | E. A. Zatsarinnaya N. V. Kolupaeva L. V. Kolupaeva |
| author_sort | E. A. Zatsarinnaya |
| collection | DOAJ |
| description | An important problem of our time is the resistance of bacteria to antimicrobial drugs. Surface water bodies accumulate all kinds of antibiotic-resistant bacteria found in the catchment area.The aim. To compare the antibiotic resistance of enterobacteria isolated from freshwater ecosystems of the Murmansk and Ryazan regions.Methods. Isolation was performed by the disk-diffusion method. For species identification, the “Rapid-entero 200 M” test system was used. Sensitivity was determined to 19 antibacterial drugs by the disk diffusion method in accordance with the requirements of MUK 4.2.1980-04 and Clinical guidelines (2014). Data interpretation was carried out using EUCAST v. 7.0 (2017) criteria and the WHONET software package.Results. In 2016, 771 isolates of enterobacteria were isolated from the water bodies of the Ryazan region, 323 isolates from the Murmansk region. The results showed that enterobacteria were found in all surveyed surface water bodies. Citrobacter (36 %), Escherichia coli (21 %) and Providencia (21 %) dominated in the Ryazan region, while Citrobacter (35 %) and Enterobacter (21 %) dominated in the Murmansk region. Enterobacteria resistant to one or more antimicrobials dominate in both regions. The phenotype of multiple drug resistance (MDR) was found in 82.62 % of isolates in Ryazan and 95.98 % in Murmansk regions. The extreme resistance phenotype (XDR) was more common among enterobacteria isolated from water bodies of the Ryazan region. In both districts, there was a fairly high level of resistance to beta-lactam antibiotics. In both regions, the quinolones were the most effective group for inhibiting the growth of enterobacteria.Conclusion. The results of the study show that the spread of antibiotic-resistant isolates of enterobacteria in freshwater ecosystems occurs everywhere, but in northern waters this process is slower. |
| format | Article |
| id | doaj-art-4befc4b29b2142feb2efbbb98797d4a6 |
| institution | Kabale University |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| publishDate | 2022-07-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj-art-4befc4b29b2142feb2efbbb98797d4a62025-08-20T03:56:54ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962022-07-017314214910.29413/ABS.2022-7.3.152351Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zonesE. A. Zatsarinnaya0N. V. Kolupaeva1L. V. Kolupaeva2Ryazan State University named after S.A. Yesenin; Institute of North Industrial Ecology Problems – Separate subdivision of the Federal State Budgetary Institution of Science of the Federal Research Center «Kola Science Center» State Research Center for Applied Biotechnology and Microbiology State Research Center for Applied Biotechnology and Microbiology An important problem of our time is the resistance of bacteria to antimicrobial drugs. Surface water bodies accumulate all kinds of antibiotic-resistant bacteria found in the catchment area.The aim. To compare the antibiotic resistance of enterobacteria isolated from freshwater ecosystems of the Murmansk and Ryazan regions.Methods. Isolation was performed by the disk-diffusion method. For species identification, the “Rapid-entero 200 M” test system was used. Sensitivity was determined to 19 antibacterial drugs by the disk diffusion method in accordance with the requirements of MUK 4.2.1980-04 and Clinical guidelines (2014). Data interpretation was carried out using EUCAST v. 7.0 (2017) criteria and the WHONET software package.Results. In 2016, 771 isolates of enterobacteria were isolated from the water bodies of the Ryazan region, 323 isolates from the Murmansk region. The results showed that enterobacteria were found in all surveyed surface water bodies. Citrobacter (36 %), Escherichia coli (21 %) and Providencia (21 %) dominated in the Ryazan region, while Citrobacter (35 %) and Enterobacter (21 %) dominated in the Murmansk region. Enterobacteria resistant to one or more antimicrobials dominate in both regions. The phenotype of multiple drug resistance (MDR) was found in 82.62 % of isolates in Ryazan and 95.98 % in Murmansk regions. The extreme resistance phenotype (XDR) was more common among enterobacteria isolated from water bodies of the Ryazan region. In both districts, there was a fairly high level of resistance to beta-lactam antibiotics. In both regions, the quinolones were the most effective group for inhibiting the growth of enterobacteria.Conclusion. The results of the study show that the spread of antibiotic-resistant isolates of enterobacteria in freshwater ecosystems occurs everywhere, but in northern waters this process is slower.https://www.actabiomedica.ru/jour/article/view/3561drug resistancebacterialenterobacteriaceaenatural bacterial isolatesfreshwater bodies |
| spellingShingle | E. A. Zatsarinnaya N. V. Kolupaeva L. V. Kolupaeva Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones Acta Biomedica Scientifica drug resistance bacterial enterobacteriaceae natural bacterial isolates freshwater bodies |
| title | Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones |
| title_full | Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones |
| title_fullStr | Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones |
| title_full_unstemmed | Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones |
| title_short | Antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones |
| title_sort | antibiotic resistance of enterobacteria isolated from freshwater bodies of different climatic zones |
| topic | drug resistance bacterial enterobacteriaceae natural bacterial isolates freshwater bodies |
| url | https://www.actabiomedica.ru/jour/article/view/3561 |
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