Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.

Medermycin and kalafungin, two antibacterial and antitumor antibiotics isolated from different streptomycetes, share an identical polyketide skeleton core. The present study reported the discovery of kalafungin in a medermycin-producing streptomycete strain for the first time. A mutant strain obtain...

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Main Authors: Jin Lü, Qiang He, Luyao Huang, Xiaofeng Cai, Wenwen Guo, Jing He, Lili Zhang, Aiying Li
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117690&type=printable
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author Jin Lü
Qiang He
Luyao Huang
Xiaofeng Cai
Wenwen Guo
Jing He
Lili Zhang
Aiying Li
author_facet Jin Lü
Qiang He
Luyao Huang
Xiaofeng Cai
Wenwen Guo
Jing He
Lili Zhang
Aiying Li
author_sort Jin Lü
collection DOAJ
description Medermycin and kalafungin, two antibacterial and antitumor antibiotics isolated from different streptomycetes, share an identical polyketide skeleton core. The present study reported the discovery of kalafungin in a medermycin-producing streptomycete strain for the first time. A mutant strain obtained through UV mutagenesis showed a 3-fold increase in the production of this antibiotic, compared to the wild type strain. Heterologous expression experiments suggested that its production was severely controlled by the gene cluster for medermycin biosynthesis. In all, these findings suggested that kalafungin and medermycin could be accumulated by the same streptomycete and share their biosynthetic pathway to some extent in this strain.
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institution DOAJ
issn 1932-6203
language English
publishDate 2015-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj-art-4bef4a0096654ec08e65bc5bcf8bc0252025-08-20T03:01:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01102e011769010.1371/journal.pone.0117690Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.Jin LüQiang HeLuyao HuangXiaofeng CaiWenwen GuoJing HeLili ZhangAiying LiMedermycin and kalafungin, two antibacterial and antitumor antibiotics isolated from different streptomycetes, share an identical polyketide skeleton core. The present study reported the discovery of kalafungin in a medermycin-producing streptomycete strain for the first time. A mutant strain obtained through UV mutagenesis showed a 3-fold increase in the production of this antibiotic, compared to the wild type strain. Heterologous expression experiments suggested that its production was severely controlled by the gene cluster for medermycin biosynthesis. In all, these findings suggested that kalafungin and medermycin could be accumulated by the same streptomycete and share their biosynthetic pathway to some extent in this strain.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117690&type=printable
spellingShingle Jin Lü
Qiang He
Luyao Huang
Xiaofeng Cai
Wenwen Guo
Jing He
Lili Zhang
Aiying Li
Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
PLoS ONE
title Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
title_full Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
title_fullStr Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
title_full_unstemmed Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
title_short Accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor-medermycin-producing streptomycete strain.
title_sort accumulation of a bioactive benzoisochromanequinone compound kalafungin by a wild type antitumor medermycin producing streptomycete strain
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117690&type=printable
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