Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus
Abstract Brown adipose tissue (BAT) is a key thermogenic organ that regulates energy expenditure through non-shivering thermogenesis. Rabbits (Oryctolagus cuniculus) are economically important livestock animals and popular model animals for medical research. However, the epigenetic mechanisms underl...
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BMC
2025-07-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-025-11868-4 |
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| author | Kun Du Qian Wu Yu-Xuan Gan Xin-Yue Tang Jia-Xin Zan Wen-Qiang Sun Gang Luo Xi-Wen Chen Dai-Hua Wang |
| author_facet | Kun Du Qian Wu Yu-Xuan Gan Xin-Yue Tang Jia-Xin Zan Wen-Qiang Sun Gang Luo Xi-Wen Chen Dai-Hua Wang |
| author_sort | Kun Du |
| collection | DOAJ |
| description | Abstract Brown adipose tissue (BAT) is a key thermogenic organ that regulates energy expenditure through non-shivering thermogenesis. Rabbits (Oryctolagus cuniculus) are economically important livestock animals and popular model animals for medical research. However, the epigenetic mechanisms underlying BAT function remain poorly understood. In this study, we performed genome-wide profiling of H3K27ac signals in BAT and white adipose tissue (WAT) of rabbits. Using chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), we identified 133,910 high-quality H3K27ac regions (peaks). The differential peaks between BATs and WATs were significantly enriched in thermogenesis-related functions and signaling pathways. An integrative analysis of H3K27ac, chromatin accessibility, and transcriptomic data identified potential strongly activated enhancers (SAEs) that regulate key thermogenic genes, including UCP1, PPARGC1A, and PDK4. The motifs of thermogenesis-related TF families, including NR, ETS, EBF, and MEF2, were significantly enriched by the distal H3K27ac regions of genes. Population resequencing data further revealed that variants within H3K27ac regions contribute to the differentiation of rabbit breeds. Comparative genomics analysis revealed 33,805 highly conserved enhancers between rabbits and humans. Notably, we discovered a potential BAT-specific enhancer RNA (eRNA) of PPARGC1A, which exhibited high chromatin accessibility and H3K27ac signals in BAT. Our findings provide a comprehensive catalog of cis-regulatory elements in rabbit adipose tissues, highlighting the critical role of conserved enhancers in regulating thermogenesis and adipose tissue plasticity, and may advance our understanding of the epigenetic regulation of BAT. |
| format | Article |
| id | doaj-art-4be9946887bd4236b2e2db470f6b1198 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-4be9946887bd4236b2e2db470f6b11982025-08-20T03:42:37ZengBMCBMC Genomics1471-21642025-07-0126111310.1186/s12864-025-11868-4Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculusKun Du0Qian Wu1Yu-Xuan Gan2Xin-Yue Tang3Jia-Xin Zan4Wen-Qiang Sun5Gang Luo6Xi-Wen Chen7Dai-Hua Wang8Technology Research Center of Modern Breeding Development, Mianyang Normal UniversityTechnology Research Center of Modern Breeding Development, Mianyang Normal UniversityTechnology Research Center of Modern Breeding Development, Mianyang Normal UniversityTechnology Research Center of Modern Breeding Development, Mianyang Normal UniversityTechnology Research Center of Modern Breeding Development, Mianyang Normal UniversityFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural UniversityCollege of Animal Science of Fujian Agricultural, Forestry UniversityTechnology Research Center of Modern Breeding Development, Mianyang Normal UniversityTechnology Research Center of Modern Breeding Development, Mianyang Normal UniversityAbstract Brown adipose tissue (BAT) is a key thermogenic organ that regulates energy expenditure through non-shivering thermogenesis. Rabbits (Oryctolagus cuniculus) are economically important livestock animals and popular model animals for medical research. However, the epigenetic mechanisms underlying BAT function remain poorly understood. In this study, we performed genome-wide profiling of H3K27ac signals in BAT and white adipose tissue (WAT) of rabbits. Using chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq), we identified 133,910 high-quality H3K27ac regions (peaks). The differential peaks between BATs and WATs were significantly enriched in thermogenesis-related functions and signaling pathways. An integrative analysis of H3K27ac, chromatin accessibility, and transcriptomic data identified potential strongly activated enhancers (SAEs) that regulate key thermogenic genes, including UCP1, PPARGC1A, and PDK4. The motifs of thermogenesis-related TF families, including NR, ETS, EBF, and MEF2, were significantly enriched by the distal H3K27ac regions of genes. Population resequencing data further revealed that variants within H3K27ac regions contribute to the differentiation of rabbit breeds. Comparative genomics analysis revealed 33,805 highly conserved enhancers between rabbits and humans. Notably, we discovered a potential BAT-specific enhancer RNA (eRNA) of PPARGC1A, which exhibited high chromatin accessibility and H3K27ac signals in BAT. Our findings provide a comprehensive catalog of cis-regulatory elements in rabbit adipose tissues, highlighting the critical role of conserved enhancers in regulating thermogenesis and adipose tissue plasticity, and may advance our understanding of the epigenetic regulation of BAT.https://doi.org/10.1186/s12864-025-11868-4Brown adipose tissuesRabbitsChIP-seqEpigeneticsTFs |
| spellingShingle | Kun Du Qian Wu Yu-Xuan Gan Xin-Yue Tang Jia-Xin Zan Wen-Qiang Sun Gang Luo Xi-Wen Chen Dai-Hua Wang Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus BMC Genomics Brown adipose tissues Rabbits ChIP-seq Epigenetics TFs |
| title | Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus |
| title_full | Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus |
| title_fullStr | Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus |
| title_full_unstemmed | Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus |
| title_short | Genome-wide identification of the H3K27ac signals reveals key thermogenic cis-regulatory elements of brown adipose tissues in Oryctolagus cuniculus |
| title_sort | genome wide identification of the h3k27ac signals reveals key thermogenic cis regulatory elements of brown adipose tissues in oryctolagus cuniculus |
| topic | Brown adipose tissues Rabbits ChIP-seq Epigenetics TFs |
| url | https://doi.org/10.1186/s12864-025-11868-4 |
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