Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.

Peroxidasin (PXDN), is a heme peroxidase with a critical role in the crosslinking of type IV collagen, being essential for basement membrane integrity. Overexpression of PXDN has been associated with poor clinical outcomes in many cancers; however, little is currently known about its role in renal c...

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Main Authors: Roberto Silva, Jorge Reis Almeida, Ana Rita Coelho, Isabel Brandão, Bárbara Gomes, Inês Soares Alencastre, João Paulo Oliveira
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Experimental and Molecular Pathology
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Online Access:http://www.sciencedirect.com/science/article/pii/S0014480025000437
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author Roberto Silva
Jorge Reis Almeida
Ana Rita Coelho
Isabel Brandão
Bárbara Gomes
Inês Soares Alencastre
João Paulo Oliveira
author_facet Roberto Silva
Jorge Reis Almeida
Ana Rita Coelho
Isabel Brandão
Bárbara Gomes
Inês Soares Alencastre
João Paulo Oliveira
author_sort Roberto Silva
collection DOAJ
description Peroxidasin (PXDN), is a heme peroxidase with a critical role in the crosslinking of type IV collagen, being essential for basement membrane integrity. Overexpression of PXDN has been associated with poor clinical outcomes in many cancers; however, little is currently known about its role in renal cell carcinoma (RCC). In this study, we characterized the expression of PXDN in tumour tissue and adjacent non-neoplastic tissue from cases of clear cell renal cell carcinoma (ccRCC), chromophobe RCC (chRCC), and renal oncocytoma using immunohistochemistry. Our results revealed increased PXDN expression in the intratumoural capillaries of ccRCC compared to other tumour types, including oncocytoma. Additionally, there was a notable increase in PXDN expression in the proximal tubular epithelial cells of non-neoplastic adjacent parenchyma in ccRCC cases compared to the same region in oncocytomas. These findings suggest, for the first time, that PXDN may play a role in tumour angiogenesis, potentially promoting metastases and malignancy. Furthermore, PXDN may be involved in the crosstalk between the tumour and adjacent tissues, a mechanism warranting further investigation.
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institution Kabale University
issn 1096-0945
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publishDate 2025-09-01
publisher Elsevier
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series Experimental and Molecular Pathology
spelling doaj-art-4be574279a0e43ddbdb00503e3d657972025-08-20T05:04:02ZengElsevierExperimental and Molecular Pathology1096-09452025-09-0114310499310.1016/j.yexmp.2025.104993Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.Roberto Silva0Jorge Reis Almeida1Ana Rita Coelho2Isabel Brandão3Bárbara Gomes4Inês Soares Alencastre5João Paulo Oliveira6University of Porto – Faculty of Medicine, Pathology Department, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.; ULS São João, EPE, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.; Corresponding author at: Departamento de Patologia, Faculdade de Medicina da Universidade do Porto, Alameda Hernâni Monteiro, 4200-319 Porto, Portugal.i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal.University of Porto – Faculty of Medicine, Pathology Department, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.; ULS São João, EPE, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal.; University of Porto – Instituto de Ciências Biomédicas Abel Salazar, R. de Jorge de Viterbo Ferreira 228, 4050-313 Porto, Portugal.ULS São João, EPE, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal.; RISE – Health, University of Porto - Faculty of Medicine, Alameda Prof. HernRISE – Healthni Monteiro, 4200-319 Porto, PortugalUniversity of Porto – Faculty of Medicine, Pathology Department, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.; ULS São João, EPE, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.; i3S – Instituto de Investigação e Inovação em Saúde, University of Porto, R. Alfredo Allen 208, 4200-135 Porto, Portugal.Peroxidasin (PXDN), is a heme peroxidase with a critical role in the crosslinking of type IV collagen, being essential for basement membrane integrity. Overexpression of PXDN has been associated with poor clinical outcomes in many cancers; however, little is currently known about its role in renal cell carcinoma (RCC). In this study, we characterized the expression of PXDN in tumour tissue and adjacent non-neoplastic tissue from cases of clear cell renal cell carcinoma (ccRCC), chromophobe RCC (chRCC), and renal oncocytoma using immunohistochemistry. Our results revealed increased PXDN expression in the intratumoural capillaries of ccRCC compared to other tumour types, including oncocytoma. Additionally, there was a notable increase in PXDN expression in the proximal tubular epithelial cells of non-neoplastic adjacent parenchyma in ccRCC cases compared to the same region in oncocytomas. These findings suggest, for the first time, that PXDN may play a role in tumour angiogenesis, potentially promoting metastases and malignancy. Furthermore, PXDN may be involved in the crosstalk between the tumour and adjacent tissues, a mechanism warranting further investigation.http://www.sciencedirect.com/science/article/pii/S0014480025000437PeroxidasinClear cell renal cell carcinomaChromophobe renal cell carcinomaOncocytomaAngiogenesis
spellingShingle Roberto Silva
Jorge Reis Almeida
Ana Rita Coelho
Isabel Brandão
Bárbara Gomes
Inês Soares Alencastre
João Paulo Oliveira
Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.
Experimental and Molecular Pathology
Peroxidasin
Clear cell renal cell carcinoma
Chromophobe renal cell carcinoma
Oncocytoma
Angiogenesis
title Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.
title_full Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.
title_fullStr Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.
title_full_unstemmed Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.
title_short Peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma.
title_sort peroxidasin expression is increased in intratumoural capillaries and in proximal tubular cells adjacent to clear cell renal cell carcinoma
topic Peroxidasin
Clear cell renal cell carcinoma
Chromophobe renal cell carcinoma
Oncocytoma
Angiogenesis
url http://www.sciencedirect.com/science/article/pii/S0014480025000437
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