Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i>
<i>Pinus elliottii</i>, a key economic conifer in southern China, requires efficient propagation methods to meet demand for elite germplasm in resin and timber production. While somatic embryogenesis-based plant regeneration has been successfully achieved in <i>Pinus elliottii</...
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2025-06-01
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| author | Lin Xu Zhaolei Deng Shan Hu Qian Liu Qifu Luan Chunxia Yang |
| author_facet | Lin Xu Zhaolei Deng Shan Hu Qian Liu Qifu Luan Chunxia Yang |
| author_sort | Lin Xu |
| collection | DOAJ |
| description | <i>Pinus elliottii</i>, a key economic conifer in southern China, requires efficient propagation methods to meet demand for elite germplasm in resin and timber production. While somatic embryogenesis-based plant regeneration has been successfully achieved in <i>Pinus elliottii</i>, large-scale production remains challenging. Our results demonstrate that the genotype of <i>Pinus elliottii</i> significantly influences the induction rate of embryogenic callus. During somatic embryo maturation, the liquid–solid induction method increased the number of mature embryos by 25.85 times. Maturation efficiency was further enhanced by a 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COS. Additionally, the incorporation of activated carbon significantly promoted both the maturation and germination of somatic embryos. In the somatic embryo maturation stage, 1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus, resulting in a total of 1452 embryos. During germination, the application of 4 g/L activated carbon achieved a germination rate of 63%, and the survival rate of somatic embryo-derived seedlings reached 85%. This study not only identifies the optimal conditions for somatic embryogenesis in <i>Pinus elliottii</i> but also establishes an efficient protocol for somatic embryo maturation induction, providing a crucial scientific foundation for the rapid propagation and seedling production of <i>Pinus elliottii</i>. |
| format | Article |
| id | doaj-art-4bdef33bd1d944099a15c6ba76ad4f3e |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-4bdef33bd1d944099a15c6ba76ad4f3e2025-08-20T03:50:17ZengMDPI AGPlants2223-77472025-06-011413198510.3390/plants14131985Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i>Lin Xu0Zhaolei Deng1Shan Hu2Qian Liu3Qifu Luan4Chunxia Yang5Jiangxi Academy of Forestry, Nanchang 330013, ChinaJiangxi Academy of Forestry, Nanchang 330013, ChinaJiangxi Academy of Forestry, Nanchang 330013, ChinaJiangxi Academy of Forestry, Nanchang 330013, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaJiangxi Academy of Forestry, Nanchang 330013, China<i>Pinus elliottii</i>, a key economic conifer in southern China, requires efficient propagation methods to meet demand for elite germplasm in resin and timber production. While somatic embryogenesis-based plant regeneration has been successfully achieved in <i>Pinus elliottii</i>, large-scale production remains challenging. Our results demonstrate that the genotype of <i>Pinus elliottii</i> significantly influences the induction rate of embryogenic callus. During somatic embryo maturation, the liquid–solid induction method increased the number of mature embryos by 25.85 times. Maturation efficiency was further enhanced by a 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COS. Additionally, the incorporation of activated carbon significantly promoted both the maturation and germination of somatic embryos. In the somatic embryo maturation stage, 1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus, resulting in a total of 1452 embryos. During germination, the application of 4 g/L activated carbon achieved a germination rate of 63%, and the survival rate of somatic embryo-derived seedlings reached 85%. This study not only identifies the optimal conditions for somatic embryogenesis in <i>Pinus elliottii</i> but also establishes an efficient protocol for somatic embryo maturation induction, providing a crucial scientific foundation for the rapid propagation and seedling production of <i>Pinus elliottii</i>.https://www.mdpi.com/2223-7747/14/13/1985<i>Pinus elliottii</i>somatic embryogenesissomatic embryo maturationhormone ratioactivated carbon |
| spellingShingle | Lin Xu Zhaolei Deng Shan Hu Qian Liu Qifu Luan Chunxia Yang Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i> Plants <i>Pinus elliottii</i> somatic embryogenesis somatic embryo maturation hormone ratio activated carbon |
| title | Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i> |
| title_full | Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i> |
| title_fullStr | Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i> |
| title_full_unstemmed | Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i> |
| title_short | Establishment of Efficient Somatic Embryo Maturation System of <i>Pinus elliottii</i> |
| title_sort | establishment of efficient somatic embryo maturation system of i pinus elliottii i |
| topic | <i>Pinus elliottii</i> somatic embryogenesis somatic embryo maturation hormone ratio activated carbon |
| url | https://www.mdpi.com/2223-7747/14/13/1985 |
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