Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily

CCCH zinc finger proteins, which are characterized by the presence of three cysteine residues and one histidine residue, play important roles in RNA processing in plants. Subfamily IX CCCH proteins were recently shown to function in stress tolerances. In this study, we analyzed CCCH IX genes in Zea...

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Main Authors: Wei-Jun Chen, Yang Zhao, Xiao-Jian Peng, Qing Dong, Jing Jin, Wei Zhou, Bei-Jiu Cheng, Qing Ma
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:International Journal of Genomics
Online Access:http://dx.doi.org/10.1155/2015/824287
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author Wei-Jun Chen
Yang Zhao
Xiao-Jian Peng
Qing Dong
Jing Jin
Wei Zhou
Bei-Jiu Cheng
Qing Ma
author_facet Wei-Jun Chen
Yang Zhao
Xiao-Jian Peng
Qing Dong
Jing Jin
Wei Zhou
Bei-Jiu Cheng
Qing Ma
author_sort Wei-Jun Chen
collection DOAJ
description CCCH zinc finger proteins, which are characterized by the presence of three cysteine residues and one histidine residue, play important roles in RNA processing in plants. Subfamily IX CCCH proteins were recently shown to function in stress tolerances. In this study, we analyzed CCCH IX genes in Zea mays, Oryza sativa, and Sorghum bicolor. These genes, which are almost intronless, were divided into four groups based on phylogenetic analysis. Microsynteny analysis revealed microsynteny in regions of some gene pairs, indicating that segmental duplication has played an important role in the expansion of this gene family. In addition, we calculated the dates of duplication by Ks analysis, finding that all microsynteny blocks were formed after the monocot-eudicot divergence. We found that deletions, multiplications, and inversions were shown to have occurred over the course of evolution. Moreover, the Ka/Ks ratios indicated that the genes in these three grass species are under strong purifying selection. Finally, we investigated the evolutionary patterns of some gene pairs conferring tolerance to abiotic stress, laying the foundation for future functional studies of these transcription factors.
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issn 2314-436X
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language English
publishDate 2015-01-01
publisher Wiley
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series International Journal of Genomics
spelling doaj-art-4bd811948bfd44139bc3ca8ec2519efa2025-08-20T02:38:42ZengWileyInternational Journal of Genomics2314-436X2314-43782015-01-01201510.1155/2015/824287824287Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene SubfamilyWei-Jun Chen0Yang Zhao1Xiao-Jian Peng2Qing Dong3Jing Jin4Wei Zhou5Bei-Jiu Cheng6Qing Ma7Key Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaKey Laboratory of Crop Biology of Anhui Province, School of Life Sciences, Anhui Agricultural University, Hefei, Anhui 230036, ChinaCCCH zinc finger proteins, which are characterized by the presence of three cysteine residues and one histidine residue, play important roles in RNA processing in plants. Subfamily IX CCCH proteins were recently shown to function in stress tolerances. In this study, we analyzed CCCH IX genes in Zea mays, Oryza sativa, and Sorghum bicolor. These genes, which are almost intronless, were divided into four groups based on phylogenetic analysis. Microsynteny analysis revealed microsynteny in regions of some gene pairs, indicating that segmental duplication has played an important role in the expansion of this gene family. In addition, we calculated the dates of duplication by Ks analysis, finding that all microsynteny blocks were formed after the monocot-eudicot divergence. We found that deletions, multiplications, and inversions were shown to have occurred over the course of evolution. Moreover, the Ka/Ks ratios indicated that the genes in these three grass species are under strong purifying selection. Finally, we investigated the evolutionary patterns of some gene pairs conferring tolerance to abiotic stress, laying the foundation for future functional studies of these transcription factors.http://dx.doi.org/10.1155/2015/824287
spellingShingle Wei-Jun Chen
Yang Zhao
Xiao-Jian Peng
Qing Dong
Jing Jin
Wei Zhou
Bei-Jiu Cheng
Qing Ma
Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily
International Journal of Genomics
title Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily
title_full Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily
title_fullStr Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily
title_full_unstemmed Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily
title_short Significant Microsynteny with New Evolutionary Highlights Is Detected through Comparative Genomic Sequence Analysis of Maize CCCH IX Gene Subfamily
title_sort significant microsynteny with new evolutionary highlights is detected through comparative genomic sequence analysis of maize ccch ix gene subfamily
url http://dx.doi.org/10.1155/2015/824287
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