Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice

Growing demand for plant-based nutraceuticals drives the need for innovative bioprocessing strategies. This study developed an integrated approach combining germination and Lactobacillus-mediated fermentation to produce a γ-aminobutyric acid (GABA)-enriched functional beverage from brown rice. Syste...

Full description

Saved in:
Bibliographic Details
Main Authors: Di Yuan, Shan Zhang, Bin Hong, Shan Shan, Jingyi Zhang, Dixin Sha, Shiwei Gao, Qing Liu, Shuwen Lu, Chuanying Ren
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/14/15/2733
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849239809912995840
author Di Yuan
Shan Zhang
Bin Hong
Shan Shan
Jingyi Zhang
Dixin Sha
Shiwei Gao
Qing Liu
Shuwen Lu
Chuanying Ren
author_facet Di Yuan
Shan Zhang
Bin Hong
Shan Shan
Jingyi Zhang
Dixin Sha
Shiwei Gao
Qing Liu
Shuwen Lu
Chuanying Ren
author_sort Di Yuan
collection DOAJ
description Growing demand for plant-based nutraceuticals drives the need for innovative bioprocessing strategies. This study developed an integrated approach combining germination and Lactobacillus-mediated fermentation to produce a γ-aminobutyric acid (GABA)-enriched functional beverage from brown rice. Systematic screening identified an optimal rice cultivar for germination. Sequential enzymatic liquefaction and saccharification were optimized to generate a suitable hydrolysate. Screening of 13 probiotic strains revealed that a 10-strain <i>Lactobacillus–Bifidobacterium</i> consortium maximized GABA synthesis (12.2 mg/100 g). Fermentation parameters were optimized to 0.25% monosodium glutamate, 4% inoculum, 10 μmol/L pyridoxine hydrochloride, 37 °C, and 24 h. The resulting beverage achieved significantly elevated GABA concentrations while exhibiting low fat (0.2 g/100 g), reduced caloric content (233.6 kJ/100 g), and high viable probiotic counts (2 × 10<sup>8</sup> CFU/g). This strategy demonstrates significant potential for the scalable production of multifunctional, plant-based nutraceuticals with targeted bioactive components.
format Article
id doaj-art-4bd08ae5c9cc4148b8f2078bfcc04bd3
institution Kabale University
issn 2304-8158
language English
publishDate 2025-08-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj-art-4bd08ae5c9cc4148b8f2078bfcc04bd32025-08-20T04:00:50ZengMDPI AGFoods2304-81582025-08-011415273310.3390/foods14152733Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown RiceDi Yuan0Shan Zhang1Bin Hong2Shan Shan3Jingyi Zhang4Dixin Sha5Shiwei Gao6Qing Liu7Shuwen Lu8Chuanying Ren9Food Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaSuihua Branch of Heilongjiang Academy Agricultural Sciences, Suihua 152001, ChinaSuihua Branch of Heilongjiang Academy Agricultural Sciences, Suihua 152001, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaFood Processing Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaGrowing demand for plant-based nutraceuticals drives the need for innovative bioprocessing strategies. This study developed an integrated approach combining germination and Lactobacillus-mediated fermentation to produce a γ-aminobutyric acid (GABA)-enriched functional beverage from brown rice. Systematic screening identified an optimal rice cultivar for germination. Sequential enzymatic liquefaction and saccharification were optimized to generate a suitable hydrolysate. Screening of 13 probiotic strains revealed that a 10-strain <i>Lactobacillus–Bifidobacterium</i> consortium maximized GABA synthesis (12.2 mg/100 g). Fermentation parameters were optimized to 0.25% monosodium glutamate, 4% inoculum, 10 μmol/L pyridoxine hydrochloride, 37 °C, and 24 h. The resulting beverage achieved significantly elevated GABA concentrations while exhibiting low fat (0.2 g/100 g), reduced caloric content (233.6 kJ/100 g), and high viable probiotic counts (2 × 10<sup>8</sup> CFU/g). This strategy demonstrates significant potential for the scalable production of multifunctional, plant-based nutraceuticals with targeted bioactive components.https://www.mdpi.com/2304-8158/14/15/2733γ-aminobutyric acid (GABA)germination<i>Lactobacillus</i> fermentationgerminated brown rice (GBR)beverage
spellingShingle Di Yuan
Shan Zhang
Bin Hong
Shan Shan
Jingyi Zhang
Dixin Sha
Shiwei Gao
Qing Liu
Shuwen Lu
Chuanying Ren
Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
Foods
γ-aminobutyric acid (GABA)
germination
<i>Lactobacillus</i> fermentation
germinated brown rice (GBR)
beverage
title Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
title_full Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
title_fullStr Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
title_full_unstemmed Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
title_short Dual Bioconversion Strategy: Synergistic Germination and <i>Lactobacillus</i> Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice
title_sort dual bioconversion strategy synergistic germination and i lactobacillus i fermentation engineering for a γ aminobutyric acid enriched beverage from brown rice
topic γ-aminobutyric acid (GABA)
germination
<i>Lactobacillus</i> fermentation
germinated brown rice (GBR)
beverage
url https://www.mdpi.com/2304-8158/14/15/2733
work_keys_str_mv AT diyuan dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT shanzhang dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT binhong dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT shanshan dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT jingyizhang dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT dixinsha dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT shiweigao dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT qingliu dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT shuwenlu dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice
AT chuanyingren dualbioconversionstrategysynergisticgerminationandilactobacillusifermentationengineeringforagaminobutyricacidenrichedbeveragefrombrownrice