Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin
Introduction. The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of me...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/1839627 |
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| author | Yong-Bin He Sheng-Yao Liu Song-Yun Deng Li-Peng Kuang Shao-Yong Xu Zhe Li Lei Xu Wei Liu Guo-Xin Ni |
| author_facet | Yong-Bin He Sheng-Yao Liu Song-Yun Deng Li-Peng Kuang Shao-Yong Xu Zhe Li Lei Xu Wei Liu Guo-Xin Ni |
| author_sort | Yong-Bin He |
| collection | DOAJ |
| description | Introduction. The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. Material and Methods. The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. Thereafter, BMSCs were subjected to 10% elongation using a Flexcell strain unit, combined with 20 IU/ml EPO. The proliferation of BMSCs was detected by Cell Counting Kit-8, colony formation assay, and cell cycle assay; meanwhile, the mRNA expression levels of Ets-1, C-myc, Ccnd1, and C-fos were detected by reverse transcription and real-time quantitative PCR (qPCR). The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. The role of the extracellular signal-regulated kinases 1/2 (ERK1/2) in the osteogenesis of BMSCs stimulated by mechanical stretch combined with 20 IU/ml EPO was examined by Western blot. Results. Our results showed that effects of EPO on BMSCs included a dose-response relationship, with the 20 IU/ml EPO yielding the largest. Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. The increase in ALP, mineral deposition, and osteoblastic genes induced by the mechanical stretch–EPO combination was inhibited by U0126, an ERK1/2 inhibitor. Conclusion. EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. The underlying mechanism may be related to the activation of the ERK1/2 signaling pathway. |
| format | Article |
| id | doaj-art-4bcf1f6dd36d45bb9c480958913d38ae |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-4bcf1f6dd36d45bb9c480958913d38ae2025-08-20T03:20:13ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/18396271839627Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by ErythropoietinYong-Bin He0Sheng-Yao Liu1Song-Yun Deng2Li-Peng Kuang3Shao-Yong Xu4Zhe Li5Lei Xu6Wei Liu7Guo-Xin Ni8School of Sport Medicine and Rehabilitation, Beijing Sport University, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Guangzhou Medical University, ChinaDepartment of Orthopeadics and Traumatology, Nanfang Hospital, Southern Medical University, ChinaDepartment of Orthopedics, The Fifth Affiliated Hospital of Zunyi Medical University, ChinaDepartment of Orthopeadics and Traumatology, Nanfang Hospital, Southern Medical University, ChinaDepartment of Orthopaedics and Traumatology, Zhengzhou Orthopaedics Hospital, Zhengzhou, ChinaDepartment of Orthopeadics and Traumatology, Nanfang Hospital, Southern Medical University, ChinaDepartment of Orthopedics, The People’s Hospital of Gaoming District of Foshan City, ChinaSchool of Sport Medicine and Rehabilitation, Beijing Sport University, ChinaIntroduction. The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. Material and Methods. The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. Thereafter, BMSCs were subjected to 10% elongation using a Flexcell strain unit, combined with 20 IU/ml EPO. The proliferation of BMSCs was detected by Cell Counting Kit-8, colony formation assay, and cell cycle assay; meanwhile, the mRNA expression levels of Ets-1, C-myc, Ccnd1, and C-fos were detected by reverse transcription and real-time quantitative PCR (qPCR). The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. The role of the extracellular signal-regulated kinases 1/2 (ERK1/2) in the osteogenesis of BMSCs stimulated by mechanical stretch combined with 20 IU/ml EPO was examined by Western blot. Results. Our results showed that effects of EPO on BMSCs included a dose-response relationship, with the 20 IU/ml EPO yielding the largest. Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. The increase in ALP, mineral deposition, and osteoblastic genes induced by the mechanical stretch–EPO combination was inhibited by U0126, an ERK1/2 inhibitor. Conclusion. EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. The underlying mechanism may be related to the activation of the ERK1/2 signaling pathway.http://dx.doi.org/10.1155/2019/1839627 |
| spellingShingle | Yong-Bin He Sheng-Yao Liu Song-Yun Deng Li-Peng Kuang Shao-Yong Xu Zhe Li Lei Xu Wei Liu Guo-Xin Ni Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin Stem Cells International |
| title | Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin |
| title_full | Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin |
| title_fullStr | Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin |
| title_full_unstemmed | Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin |
| title_short | Mechanical Stretch Promotes the Osteogenic Differentiation of Bone Mesenchymal Stem Cells Induced by Erythropoietin |
| title_sort | mechanical stretch promotes the osteogenic differentiation of bone mesenchymal stem cells induced by erythropoietin |
| url | http://dx.doi.org/10.1155/2019/1839627 |
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