Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep
The melanocortin receptor 1 (MC1R) plays a central role in regulation of animal coat color formation. In this study, we sequenced the complete coding region and parts of the 5′- and 3′-untranslated regions of the MC1R gene in Chinese sheep with completely white (Large-tail...
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/675382 |
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| author | Guang-Li Yang Dong-Li Fu Xia Lang Yu-Tao Wang Shu-Ru Cheng Su-Li Fang Yu-Zhu Luo |
| author_facet | Guang-Li Yang Dong-Li Fu Xia Lang Yu-Tao Wang Shu-Ru Cheng Su-Li Fang Yu-Zhu Luo |
| author_sort | Guang-Li Yang |
| collection | DOAJ |
| description | The melanocortin receptor 1 (MC1R) plays a central role in regulation of animal coat color formation. In this study, we sequenced the complete coding region
and parts of the 5′- and 3′-untranslated regions of the MC1R gene in Chinese sheep with completely
white (Large-tailed Han sheep), black (Minxian Black-fur sheep), and brown coat colors (Kazakh Fat-Rumped sheep). The results showed five single nucleotide
polymorphisms (SNPs): two non-synonymous mutations previously associated with coat color (c.218 T>A, p.73 Met>Lys. c.361 G>A, p.121 Asp>Asn)
and three synonymous mutations (c.429 C>T, p.143 Tyr>Tyr; c.600 T>G, p.200 Leu>Leu. c.735 C>T, p.245 Ile>Ile). Meanwhile, all mutations
were detected in Minxian Black-fur sheep. However, the two nonsynonymous mutation sites were not in all studied breeds (Large-tailed Han, Small-tailed Han, Gansu Alpine Merino,
and China Merino breeds), all of which are in white coat. A single haplotype AATGT (haplotype3) was uniquely associated with black coat color in Minxian Black-fur breed (P=9.72E-72, chi-square test). The first and second A alleles in this haplotype 3 represent location at 218 and 361 positions, respectively. Our results suggest that the mutations
of MC1R gene are associated with black coat color phenotype in Chinese sheep. |
| format | Article |
| id | doaj-art-4bc6da9331b541a983b7348d828f3a55 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4bc6da9331b541a983b7348d828f3a552025-08-20T02:38:42ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/675382675382Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese SheepGuang-Li Yang0Dong-Li Fu1Xia Lang2Yu-Tao Wang3Shu-Ru Cheng4Su-Li Fang5Yu-Zhu Luo6Department of Life Sciences, Shangqiu Normal University, Shangqiu 476000, ChinaGansu Province Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, ChinaLanzhou Institute of Animal and Veterinary Pharmaceutics Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaGansu Province Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Province Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Province Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, ChinaGansu Province Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou 730070, ChinaThe melanocortin receptor 1 (MC1R) plays a central role in regulation of animal coat color formation. In this study, we sequenced the complete coding region and parts of the 5′- and 3′-untranslated regions of the MC1R gene in Chinese sheep with completely white (Large-tailed Han sheep), black (Minxian Black-fur sheep), and brown coat colors (Kazakh Fat-Rumped sheep). The results showed five single nucleotide polymorphisms (SNPs): two non-synonymous mutations previously associated with coat color (c.218 T>A, p.73 Met>Lys. c.361 G>A, p.121 Asp>Asn) and three synonymous mutations (c.429 C>T, p.143 Tyr>Tyr; c.600 T>G, p.200 Leu>Leu. c.735 C>T, p.245 Ile>Ile). Meanwhile, all mutations were detected in Minxian Black-fur sheep. However, the two nonsynonymous mutation sites were not in all studied breeds (Large-tailed Han, Small-tailed Han, Gansu Alpine Merino, and China Merino breeds), all of which are in white coat. A single haplotype AATGT (haplotype3) was uniquely associated with black coat color in Minxian Black-fur breed (P=9.72E-72, chi-square test). The first and second A alleles in this haplotype 3 represent location at 218 and 361 positions, respectively. Our results suggest that the mutations of MC1R gene are associated with black coat color phenotype in Chinese sheep.http://dx.doi.org/10.1155/2013/675382 |
| spellingShingle | Guang-Li Yang Dong-Li Fu Xia Lang Yu-Tao Wang Shu-Ru Cheng Su-Li Fang Yu-Zhu Luo Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep The Scientific World Journal |
| title | Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep |
| title_full | Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep |
| title_fullStr | Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep |
| title_full_unstemmed | Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep |
| title_short | Mutations in MC1R Gene Determine Black Coat Color Phenotype in Chinese Sheep |
| title_sort | mutations in mc1r gene determine black coat color phenotype in chinese sheep |
| url | http://dx.doi.org/10.1155/2013/675382 |
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