B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody
P3 is a murine, germline, IgM mAb that recognizes N-glycolylated gangliosides and other self-antigens. This antibody is able to induce an anti-idiotypic IgG response and B-T idiotypic cascade, even in the absence of any adjuvant or carrier protein. P3 mAb immunization induces the expression of activ...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/2860867 |
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| author | Darel Martínez Amaury Pupo Lianet Cabrera Judith Raymond Nichol E. Holodick Ana María Hernández |
| author_facet | Darel Martínez Amaury Pupo Lianet Cabrera Judith Raymond Nichol E. Holodick Ana María Hernández |
| author_sort | Darel Martínez |
| collection | DOAJ |
| description | P3 is a murine, germline, IgM mAb that recognizes N-glycolylated gangliosides and other self-antigens. This antibody is able to induce an anti-idiotypic IgG response and B-T idiotypic cascade, even in the absence of any adjuvant or carrier protein. P3 mAb immunization induces the expression of activation markers in a significant percentage of B-1a cells in vivo. Interestingly, transfer of both B-1a and B-2 to BALB/Xid mice was required to recover anti-P3 IgG response in this model. In fact, P3 mAb activated B-2 cells, in vitro, inducing secretion of IFN-γ and IL-4, although this activation was not detected ex vivo. Interestingly, naïve CD8+ T cells increased the expression of activation markers and IFN-γ secretion in the presence of B-1a cells isolated from P3 mAb-immunized mice, even without in vitro restimulation. In contrast, B-2 cells were able to stimulate CD8+ T cells only if P3 was added in vitro. Using bioinformatics, a MHC class I-binding peptide from P3 VH region was identified. P3 mAb was able to induce a specific CTL response in vivo against cells presenting this peptide. Both humoral and CTL anti-idiotypic responses could be mechanisms to protect against the self-reactive antibody, contributing to keeping the tolerance to self-antigens. |
| format | Article |
| id | doaj-art-4bc5ddab6ff3485db5da05270d54eb98 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-4bc5ddab6ff3485db5da05270d54eb982025-02-03T01:03:22ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/28608672860867B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-AntibodyDarel Martínez0Amaury Pupo1Lianet Cabrera2Judith Raymond3Nichol E. Holodick4Ana María Hernández5Tumor Immunology Direction, Center of Molecular Immunology, Havana, CubaSystems Biology Direction, Center of Molecular Immunology, Havana, CubaTumor Immunology Direction, Center of Molecular Immunology, Havana, CubaSystems Biology Direction, Center of Molecular Immunology, Havana, CubaImmunobiology Laboratory, Center for Oncology and Cell Biology, The Feinstein Institute for Medical Research, New York, NY, USATumor Immunology Direction, Center of Molecular Immunology, Havana, CubaP3 is a murine, germline, IgM mAb that recognizes N-glycolylated gangliosides and other self-antigens. This antibody is able to induce an anti-idiotypic IgG response and B-T idiotypic cascade, even in the absence of any adjuvant or carrier protein. P3 mAb immunization induces the expression of activation markers in a significant percentage of B-1a cells in vivo. Interestingly, transfer of both B-1a and B-2 to BALB/Xid mice was required to recover anti-P3 IgG response in this model. In fact, P3 mAb activated B-2 cells, in vitro, inducing secretion of IFN-γ and IL-4, although this activation was not detected ex vivo. Interestingly, naïve CD8+ T cells increased the expression of activation markers and IFN-γ secretion in the presence of B-1a cells isolated from P3 mAb-immunized mice, even without in vitro restimulation. In contrast, B-2 cells were able to stimulate CD8+ T cells only if P3 was added in vitro. Using bioinformatics, a MHC class I-binding peptide from P3 VH region was identified. P3 mAb was able to induce a specific CTL response in vivo against cells presenting this peptide. Both humoral and CTL anti-idiotypic responses could be mechanisms to protect against the self-reactive antibody, contributing to keeping the tolerance to self-antigens.http://dx.doi.org/10.1155/2017/2860867 |
| spellingShingle | Darel Martínez Amaury Pupo Lianet Cabrera Judith Raymond Nichol E. Holodick Ana María Hernández B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody Journal of Immunology Research |
| title | B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody |
| title_full | B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody |
| title_fullStr | B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody |
| title_full_unstemmed | B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody |
| title_short | B-CD8+ T Cell Interactions in the Anti-Idiotypic Response against a Self-Antibody |
| title_sort | b cd8 t cell interactions in the anti idiotypic response against a self antibody |
| url | http://dx.doi.org/10.1155/2017/2860867 |
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