Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model
Objective(s): Traditionally, Bee venom (BV) is used through stinging or injection to treat rheumatoid arthritis (RA). This study aimed to assess the side effects of local bee venom nanoemulsions (BV-NEs) in the collagen-induced arthritis (CIA) model by examining biochemical and hematological paramet...
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Mashhad University of Medical Sciences
2024-10-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_24458_822de299471530272c02d007897b249c.pdf |
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| author | Yaser Yousefpoor Amir Amani Seyyed Mohammad Hashemi Ali Afgar Seyed Mohammad Amini Mohamad Javad Mirzaei-Parsa |
| author_facet | Yaser Yousefpoor Amir Amani Seyyed Mohammad Hashemi Ali Afgar Seyed Mohammad Amini Mohamad Javad Mirzaei-Parsa |
| author_sort | Yaser Yousefpoor |
| collection | DOAJ |
| description | Objective(s): Traditionally, Bee venom (BV) is used through stinging or injection to treat rheumatoid arthritis (RA). This study aimed to assess the side effects of local bee venom nanoemulsions (BV-NEs) in the collagen-induced arthritis (CIA) model by examining biochemical and hematological parameters. Materials and Methods: The BV-NEs were prepared, and the CIA model was induced in rats. After the seventh day, the groups were locally treated for two weeks as the following: blank (free treatment), negative control (NE-0), positive control (hydrocortisone acetate ointment 1%, 50 mg/day), BV control (37.5 µg/ml/day), and BV-NEs receiving 75, 37.5, 18.75, and 9.37 µg/ml/day. Three steps of blood sampling were done on days 0, 7, and 21 (healthy rats, before treatment, and at the end of treatment, respectively).Results: The results revealed that blood levels of Glucose, Cholesterol, Urea, Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), White blood cell (WBC), and %Neutrophil significantly increased before the treatment. Nevertheless, most parameters declined at the end of the treatment compared to the blank and negative control groups about BV-NEs dose-dependently. The drastic changes in biochemical parameters in the CIA model indicated the effect of the immune system function on the metabolic system. Also, NE’s impact of BV passed through the skin on these items. Conclusion: BV-NEs can reduce inflammation caused by arthritis without acute adverse effects on the routine biochemical and hematological parameters. |
| format | Article |
| id | doaj-art-4bba578ccb5f40058d7528de5ece4d89 |
| institution | Kabale University |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-4bba578ccb5f40058d7528de5ece4d892025-08-20T03:58:40ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042024-10-0111441742510.22038/nmj.2024.75993.185024458Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis modelYaser Yousefpoor0Amir Amani1Seyyed Mohammad Hashemi2Ali Afgar3Seyed Mohammad Amini4Mohamad Javad Mirzaei-Parsa5Department of Medical Biotechnology, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, IranNatural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, IranKhalil Abad Health Center, Mashhad University of Medical Sciences, Mashhad, IranResearch Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, IranRadiation Biology Research Center Iran University of Medical Sciences (IUMS) Tehran, IranStem Cells and Regenerative Medicine Innovation Center, Kerman University of Medical Sciences, Kerman, IranObjective(s): Traditionally, Bee venom (BV) is used through stinging or injection to treat rheumatoid arthritis (RA). This study aimed to assess the side effects of local bee venom nanoemulsions (BV-NEs) in the collagen-induced arthritis (CIA) model by examining biochemical and hematological parameters. Materials and Methods: The BV-NEs were prepared, and the CIA model was induced in rats. After the seventh day, the groups were locally treated for two weeks as the following: blank (free treatment), negative control (NE-0), positive control (hydrocortisone acetate ointment 1%, 50 mg/day), BV control (37.5 µg/ml/day), and BV-NEs receiving 75, 37.5, 18.75, and 9.37 µg/ml/day. Three steps of blood sampling were done on days 0, 7, and 21 (healthy rats, before treatment, and at the end of treatment, respectively).Results: The results revealed that blood levels of Glucose, Cholesterol, Urea, Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), White blood cell (WBC), and %Neutrophil significantly increased before the treatment. Nevertheless, most parameters declined at the end of the treatment compared to the blank and negative control groups about BV-NEs dose-dependently. The drastic changes in biochemical parameters in the CIA model indicated the effect of the immune system function on the metabolic system. Also, NE’s impact of BV passed through the skin on these items. Conclusion: BV-NEs can reduce inflammation caused by arthritis without acute adverse effects on the routine biochemical and hematological parameters.https://nmj.mums.ac.ir/article_24458_822de299471530272c02d007897b249c.pdfbee venombiochemical markershematological testsnanoemulsioncollagen induced arthritis |
| spellingShingle | Yaser Yousefpoor Amir Amani Seyyed Mohammad Hashemi Ali Afgar Seyed Mohammad Amini Mohamad Javad Mirzaei-Parsa Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model Nanomedicine Journal bee venom biochemical markers hematological tests nanoemulsion collagen induced arthritis |
| title | Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model |
| title_full | Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model |
| title_fullStr | Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model |
| title_full_unstemmed | Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model |
| title_short | Blood and biochemical changes caused by bee venom-nanoemulsions; a study on animal arthritis model |
| title_sort | blood and biochemical changes caused by bee venom nanoemulsions a study on animal arthritis model |
| topic | bee venom biochemical markers hematological tests nanoemulsion collagen induced arthritis |
| url | https://nmj.mums.ac.ir/article_24458_822de299471530272c02d007897b249c.pdf |
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