Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury
Objective(s): In our study, the neuroprotective efficacy of pre- and post-traumatic applications of boric acid (BA) in rats with experimentally induced spinal cord injury (SCI) was investigated.Materials and Methods: The experimental animals were divided into four groups: control group (C), SCI grou...
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Mashhad University of Medical Sciences
2025-04-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_25270_906d8b8db45921ac4cd6d74ccc456e27.pdf |
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| author | Turan Kandemir İbrahim Söğüt Zeki Ataizi Betül Can Ayşegül Oğlakçı İlhan Dilek Burukoglu Donmez Güngör Kanbak |
| author_facet | Turan Kandemir İbrahim Söğüt Zeki Ataizi Betül Can Ayşegül Oğlakçı İlhan Dilek Burukoglu Donmez Güngör Kanbak |
| author_sort | Turan Kandemir |
| collection | DOAJ |
| description | Objective(s): In our study, the neuroprotective efficacy of pre- and post-traumatic applications of boric acid (BA) in rats with experimentally induced spinal cord injury (SCI) was investigated.Materials and Methods: The experimental animals were divided into four groups: control group (C), SCI group (SCI), BA-treated group before SCI (BA+SCI), and BA-treated group after SCI (SCI+BA). Forty-eight hours after SCI, biochemical levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and cytochrome c (Cytc) and caspase-3 (Casp3) expressions were measured in the spinal cord tissues and were examined histologically.Results: After SCI, oxidative stress markers, such as MDA, TOS, and OSI, and apoptosis markers Cytc and Casp3 showed an increase in levels compared to Group C. The oxidative stress markers that increased after SCI decreased with BA+SCI application, while Cytc level, one of the apoptosis markers that increased after SCI, decreased in both groups with BA application. Cell, myelin, ependymal damage, and hemorrhage levels increased after SCI compared to Group C. These histological markers increased after SCI and decreased after BA+SCI. BA was found to reduce SCI-induced oxidative stress and oxidative stress-induced apoptosis.Conclusion: BA administered before SCI was shown to be more effective in protecting neural damage. |
| format | Article |
| id | doaj-art-4bb9c59916ea41b08063dcc01912a99d |
| institution | DOAJ |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-4bb9c59916ea41b08063dcc01912a99d2025-08-20T03:08:50ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-04-0128444445010.22038/ijbms.2024.81531.1764925270Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injuryTuran Kandemir0İbrahim Söğüt1Zeki Ataizi2Betül Can3Ayşegül Oğlakçı İlhan4Dilek Burukoglu Donmez5Güngör Kanbak6Department of Neurosurgery, Eskisehir Osmangazi University Faculty of Medicine, Eskisehir, TurkeyDepartment of Biochemistry, Demiroglu Bilim University, Medical Faculty, Istanbul, TurkeyDepartment of Neurosurgery, Eskişehir Yunus Emre StateDepartment of Medical Biochemistry, Eskisehir Osmangazi University Faculty of Medicine, Eskisehir, TurkeyDepartment of Medical Laboratory Techniques, Eldivan Health Services Vocational School, Cankırı Karatekin University, Cankırı, TurkeyDepartment of Histology and Embryology, Eskisehir Osmangazi University Faculty of Medicine, Eskisehir, TurkeyDepartment of Medical Biochemistry, Eskisehir Osmangazi University Faculty of Medicine, Eskisehir, TurkeyObjective(s): In our study, the neuroprotective efficacy of pre- and post-traumatic applications of boric acid (BA) in rats with experimentally induced spinal cord injury (SCI) was investigated.Materials and Methods: The experimental animals were divided into four groups: control group (C), SCI group (SCI), BA-treated group before SCI (BA+SCI), and BA-treated group after SCI (SCI+BA). Forty-eight hours after SCI, biochemical levels of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), and cytochrome c (Cytc) and caspase-3 (Casp3) expressions were measured in the spinal cord tissues and were examined histologically.Results: After SCI, oxidative stress markers, such as MDA, TOS, and OSI, and apoptosis markers Cytc and Casp3 showed an increase in levels compared to Group C. The oxidative stress markers that increased after SCI decreased with BA+SCI application, while Cytc level, one of the apoptosis markers that increased after SCI, decreased in both groups with BA application. Cell, myelin, ependymal damage, and hemorrhage levels increased after SCI compared to Group C. These histological markers increased after SCI and decreased after BA+SCI. BA was found to reduce SCI-induced oxidative stress and oxidative stress-induced apoptosis.Conclusion: BA administered before SCI was shown to be more effective in protecting neural damage.https://ijbms.mums.ac.ir/article_25270_906d8b8db45921ac4cd6d74ccc456e27.pdfapoptosisboric acidneuroprotectiveoxidative stressspinal cord injury |
| spellingShingle | Turan Kandemir İbrahim Söğüt Zeki Ataizi Betül Can Ayşegül Oğlakçı İlhan Dilek Burukoglu Donmez Güngör Kanbak Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury Iranian Journal of Basic Medical Sciences apoptosis boric acid neuroprotective oxidative stress spinal cord injury |
| title | Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury |
| title_full | Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury |
| title_fullStr | Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury |
| title_full_unstemmed | Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury |
| title_short | Pre- and post-traumatic boric acid therapy prevents oxidative stress-mediated neuronal apoptosis in spinal cord injury |
| title_sort | pre and post traumatic boric acid therapy prevents oxidative stress mediated neuronal apoptosis in spinal cord injury |
| topic | apoptosis boric acid neuroprotective oxidative stress spinal cord injury |
| url | https://ijbms.mums.ac.ir/article_25270_906d8b8db45921ac4cd6d74ccc456e27.pdf |
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