Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat
Stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and robust resistance...
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2025-05-01
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| author | Chengzhi Jiang Yujie Luo Doudou Huang Meiling Chen Ennian Yang Guangrong Li Zujun Yang |
| author_facet | Chengzhi Jiang Yujie Luo Doudou Huang Meiling Chen Ennian Yang Guangrong Li Zujun Yang |
| author_sort | Chengzhi Jiang |
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| description | Stripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and robust resistance genes from the relatives of wheat. <i>Thinopyrum intermedium</i> (Host) Barkworth and D.R. Dewey has been widely hybridized with common wheat and has been shown to be a valuable source of genes, conferring resistance and tolerance against both the biotic and abiotic stresses affecting wheat. In this study, a novel wheat–<i>Th. intermedium</i> 2StS.2J<sup>S</sup>L addition line, named Th93-1-6, which originated from wheat–<i>Th. intermedium</i> partial amphidiploid line, Th24-19-5, was comprehensively characterized using nondenaturing-fluorescence <i>in situ</i> hybridization (ND-FISH) and Oligo-FISH painting techniques. To detect plants with the transfer of resistance genes from Th93-1-6 to wheat chromosomes, 2384 M<sub>1</sub>-M<sub>3</sub> plants from the cross between Th93-1-6 and the susceptible wheat cultivar MY11 were studied by ND-FISH using multiple probes. A total of 37 types of 2StS.2J<sup>S</sup>L chromosomal aberrations were identified. Subsequently, 12 homozygous lines were developed to construct a cytological bin map. Ten chromosomal bins on the 2StS.2J<sup>S</sup>L chromosome were constructed based on 84 specific molecular markers. Among them, eight alien chromosome aberration lines, which all contained the bin 2StS-3, showed enhanced stripe rust resistance. Consequently, the gene(s) for stripe rust resistance was physically mapped to the 92.88-155.32 Mb region of 2StS in <i>Thinopyrum intermedium</i> reference genome sequences v2.1. Moreover, these newly developed wheat–<i>Th. intermedium</i> 2StS.2J<sup>S</sup>L translocation lines are expected to serve as valuable genetic resources in the breeding of rust-resistant wheat cultivars. |
| format | Article |
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| spelling | doaj-art-4bb7fefa31ff4ba6bb8039ca8d8e9c442025-08-20T02:33:51ZengMDPI AGPlants2223-77472025-05-011410153810.3390/plants14101538Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in WheatChengzhi Jiang0Yujie Luo1Doudou Huang2Meiling Chen3Ennian Yang4Guangrong Li5Zujun Yang6School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaCrop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, ChinaStripe rust, caused by <i>Puccinia striiformis</i> f. sp. <i>tritici</i>, is a highly destructive disease prevalent across most wheat-growing regions globally. The most effective strategy for combating this disease is through the exploitation of durable and robust resistance genes from the relatives of wheat. <i>Thinopyrum intermedium</i> (Host) Barkworth and D.R. Dewey has been widely hybridized with common wheat and has been shown to be a valuable source of genes, conferring resistance and tolerance against both the biotic and abiotic stresses affecting wheat. In this study, a novel wheat–<i>Th. intermedium</i> 2StS.2J<sup>S</sup>L addition line, named Th93-1-6, which originated from wheat–<i>Th. intermedium</i> partial amphidiploid line, Th24-19-5, was comprehensively characterized using nondenaturing-fluorescence <i>in situ</i> hybridization (ND-FISH) and Oligo-FISH painting techniques. To detect plants with the transfer of resistance genes from Th93-1-6 to wheat chromosomes, 2384 M<sub>1</sub>-M<sub>3</sub> plants from the cross between Th93-1-6 and the susceptible wheat cultivar MY11 were studied by ND-FISH using multiple probes. A total of 37 types of 2StS.2J<sup>S</sup>L chromosomal aberrations were identified. Subsequently, 12 homozygous lines were developed to construct a cytological bin map. Ten chromosomal bins on the 2StS.2J<sup>S</sup>L chromosome were constructed based on 84 specific molecular markers. Among them, eight alien chromosome aberration lines, which all contained the bin 2StS-3, showed enhanced stripe rust resistance. Consequently, the gene(s) for stripe rust resistance was physically mapped to the 92.88-155.32 Mb region of 2StS in <i>Thinopyrum intermedium</i> reference genome sequences v2.1. Moreover, these newly developed wheat–<i>Th. intermedium</i> 2StS.2J<sup>S</sup>L translocation lines are expected to serve as valuable genetic resources in the breeding of rust-resistant wheat cultivars.https://www.mdpi.com/2223-7747/14/10/1538<i>Thinopyrum intermedium</i>ND-FISHmolecular markersstripe rust resistance |
| spellingShingle | Chengzhi Jiang Yujie Luo Doudou Huang Meiling Chen Ennian Yang Guangrong Li Zujun Yang Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat Plants <i>Thinopyrum intermedium</i> ND-FISH molecular markers stripe rust resistance |
| title | Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat |
| title_full | Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat |
| title_fullStr | Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat |
| title_full_unstemmed | Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat |
| title_short | Characterization of a New Stripe Rust Resistance Gene on Chromosome 2StS from <i>Thinopyrum intermedium</i> in Wheat |
| title_sort | characterization of a new stripe rust resistance gene on chromosome 2sts from i thinopyrum intermedium i in wheat |
| topic | <i>Thinopyrum intermedium</i> ND-FISH molecular markers stripe rust resistance |
| url | https://www.mdpi.com/2223-7747/14/10/1538 |
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