Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy.
The assimilation, incorporation, and metabolism of sulfur is a fundamental process across all domains of life, yet how cells deal with varying sulfur availability is not well understood. We studied an unresolved conundrum of sulfur fixation in yeast, in which organosulfur auxotrophy caused by deleti...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-12-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001912&type=printable |
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| author | Jason S L Yu Benjamin M Heineike Johannes Hartl Simran K Aulakh Clara Correia-Melo Andrea Lehmann Oliver Lemke Federica Agostini Cory T Lee Vadim Demichev Christoph B Messner Michael Mülleder Markus Ralser |
| author_facet | Jason S L Yu Benjamin M Heineike Johannes Hartl Simran K Aulakh Clara Correia-Melo Andrea Lehmann Oliver Lemke Federica Agostini Cory T Lee Vadim Demichev Christoph B Messner Michael Mülleder Markus Ralser |
| author_sort | Jason S L Yu |
| collection | DOAJ |
| description | The assimilation, incorporation, and metabolism of sulfur is a fundamental process across all domains of life, yet how cells deal with varying sulfur availability is not well understood. We studied an unresolved conundrum of sulfur fixation in yeast, in which organosulfur auxotrophy caused by deletion of the homocysteine synthase Met17p is overcome when cells are inoculated at high cell density. In combining the use of self-establishing metabolically cooperating (SeMeCo) communities with proteomic, genetic, and biochemical approaches, we discovered an uncharacterized gene product YLL058Wp, herein named Hydrogen Sulfide Utilizing-1 (HSU1). Hsu1p acts as a homocysteine synthase and allows the cells to substitute for Met17p by reassimilating hydrosulfide ions leaked from met17Δ cells into O-acetyl-homoserine and forming homocysteine. Our results show that cells can cooperate to achieve sulfur fixation, indicating that the collective properties of microbial communities facilitate their basic metabolic capacity to overcome sulfur limitation. |
| format | Article |
| id | doaj-art-4bafcd4cc0e6402c9e4cfcf9d4e05d8c |
| institution | Kabale University |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-4bafcd4cc0e6402c9e4cfcf9d4e05d8c2025-08-20T03:25:34ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852022-12-012012e300191210.1371/journal.pbio.3001912Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy.Jason S L YuBenjamin M HeineikeJohannes HartlSimran K AulakhClara Correia-MeloAndrea LehmannOliver LemkeFederica AgostiniCory T LeeVadim DemichevChristoph B MessnerMichael MüllederMarkus RalserThe assimilation, incorporation, and metabolism of sulfur is a fundamental process across all domains of life, yet how cells deal with varying sulfur availability is not well understood. We studied an unresolved conundrum of sulfur fixation in yeast, in which organosulfur auxotrophy caused by deletion of the homocysteine synthase Met17p is overcome when cells are inoculated at high cell density. In combining the use of self-establishing metabolically cooperating (SeMeCo) communities with proteomic, genetic, and biochemical approaches, we discovered an uncharacterized gene product YLL058Wp, herein named Hydrogen Sulfide Utilizing-1 (HSU1). Hsu1p acts as a homocysteine synthase and allows the cells to substitute for Met17p by reassimilating hydrosulfide ions leaked from met17Δ cells into O-acetyl-homoserine and forming homocysteine. Our results show that cells can cooperate to achieve sulfur fixation, indicating that the collective properties of microbial communities facilitate their basic metabolic capacity to overcome sulfur limitation.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001912&type=printable |
| spellingShingle | Jason S L Yu Benjamin M Heineike Johannes Hartl Simran K Aulakh Clara Correia-Melo Andrea Lehmann Oliver Lemke Federica Agostini Cory T Lee Vadim Demichev Christoph B Messner Michael Mülleder Markus Ralser Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy. PLoS Biology |
| title | Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy. |
| title_full | Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy. |
| title_fullStr | Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy. |
| title_full_unstemmed | Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy. |
| title_short | Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy. |
| title_sort | inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001912&type=printable |
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