Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis
Background: Cultivated peanut (Arachis hypogaea L.) is a major oil and economic crop. Pod size is one of the important agronomic traits of peanut variety, with a direct impact on peanut yield. Methods:...
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IMR Press
2025-06-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL38940 |
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| author | Penghui Miao Xuanlin Li Yongshan Wan Kun Zhang Haiyang Yu Yuying Li Huadong Li Hui Yang Lu Luo Fengzhen Liu |
| author_facet | Penghui Miao Xuanlin Li Yongshan Wan Kun Zhang Haiyang Yu Yuying Li Huadong Li Hui Yang Lu Luo Fengzhen Liu |
| author_sort | Penghui Miao |
| collection | DOAJ |
| description | Background: Cultivated peanut (Arachis hypogaea L.) is a major oil and economic crop. Pod size is one of the important agronomic traits of peanut variety, with a direct impact on peanut yield. Methods: In a previous study, AhRUVBL2 was identified by map-based cloning technology as a candidate gene that regulates peanut pod size. Results: Overexpression of AhRUVBL2 in transgenic Arabidopsis significantly increased plant height, branch number, leaf size, silique size, seed size, and thousand-seed weight. Further examination revealed an increase in the area of silique exocarp cells, and the number and area of endocarp lignified cells. A total of 337 differentially expressed genes, including PRX (Periaxin) , SAUR (Small Auxin-up RNA), and PYL (Pyrabactin Resistance 1-like), were identified by transcriptome analysis of transgenic Arabidopsis silique. proAhRUVBL2-GUS was found to be expressed explicitly in seeds, and the expression activity of proAhRUVBL2-D893 was significantly greater than that of proAhRUVBL2-79266. Exogenous ABA (abscisic acid) and IAA (indole acetic acid) treatment of proAhRUVBL2-GUS-transformed tobacco leaves revealed that the AhRUVBL2 promoter was hormone-responsive. Conclusions: This study sheds light on the function of AhRUVBL2 in regulating plant growth and development. Moreover, characterization of the AhRUVBL2 promoter provides a valuable genetic resource for enhancing peanut yield. |
| format | Article |
| id | doaj-art-4ba13b9bba084d8487a1f3e533d838f3 |
| institution | Kabale University |
| issn | 2768-6701 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj-art-4ba13b9bba084d8487a1f3e533d838f32025-08-20T03:28:59ZengIMR PressFrontiers in Bioscience-Landmark2768-67012025-06-013063894010.31083/FBL38940S2768-6701(25)01745-9Study of the Function and Expression of the AhRUVBL2 Promoter in ArabidopsisPenghui Miao0Xuanlin Li1Yongshan Wan2Kun Zhang3Haiyang Yu4Yuying Li5Huadong Li6Hui Yang7Lu Luo8Fengzhen Liu9College of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaCollege of Agronomy, Shandong Agricultural University, 271018 Tai'an, Shandong, ChinaBackground: Cultivated peanut (Arachis hypogaea L.) is a major oil and economic crop. Pod size is one of the important agronomic traits of peanut variety, with a direct impact on peanut yield. Methods: In a previous study, AhRUVBL2 was identified by map-based cloning technology as a candidate gene that regulates peanut pod size. Results: Overexpression of AhRUVBL2 in transgenic Arabidopsis significantly increased plant height, branch number, leaf size, silique size, seed size, and thousand-seed weight. Further examination revealed an increase in the area of silique exocarp cells, and the number and area of endocarp lignified cells. A total of 337 differentially expressed genes, including PRX (Periaxin) , SAUR (Small Auxin-up RNA), and PYL (Pyrabactin Resistance 1-like), were identified by transcriptome analysis of transgenic Arabidopsis silique. proAhRUVBL2-GUS was found to be expressed explicitly in seeds, and the expression activity of proAhRUVBL2-D893 was significantly greater than that of proAhRUVBL2-79266. Exogenous ABA (abscisic acid) and IAA (indole acetic acid) treatment of proAhRUVBL2-GUS-transformed tobacco leaves revealed that the AhRUVBL2 promoter was hormone-responsive. Conclusions: This study sheds light on the function of AhRUVBL2 in regulating plant growth and development. Moreover, characterization of the AhRUVBL2 promoter provides a valuable genetic resource for enhancing peanut yield.https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL38940peanutahruvbl2promoterexpression characteristicspod size |
| spellingShingle | Penghui Miao Xuanlin Li Yongshan Wan Kun Zhang Haiyang Yu Yuying Li Huadong Li Hui Yang Lu Luo Fengzhen Liu Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis Frontiers in Bioscience-Landmark peanut ahruvbl2 promoter expression characteristics pod size |
| title | Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis |
| title_full | Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis |
| title_fullStr | Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis |
| title_full_unstemmed | Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis |
| title_short | Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis |
| title_sort | study of the function and expression of the ahruvbl2 promoter in arabidopsis |
| topic | peanut ahruvbl2 promoter expression characteristics pod size |
| url | https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL38940 |
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