Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis

Study of the Function and Expression of the AhRUVBL2 Promoter in Arabidopsis

Background: Cultivated peanut (Arachis hypogaea L.) is a major oil and economic crop. Pod size is one of the important agronomic traits of peanut variety, with a direct impact on peanut yield. Methods:...

Full description

Saved in:

Bibliographic Details
Main Authors:	Penghui Miao, Xuanlin Li, Yongshan Wan, Kun Zhang, Haiyang Yu, Yuying Li, Huadong Li, Hui Yang, Lu Luo, Fengzhen Liu
Format:	Article
Language:	English
Published:	IMR Press 2025-06-01
Series:	Frontiers in Bioscience-Landmark
Subjects:	peanut ahruvbl2 promoter expression characteristics pod size
Online Access:	https://www.imrpress.com/journal/FBL/30/6/10.31083/FBL38940
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Background: Cultivated peanut (Arachis hypogaea L.) is a major oil and economic crop. Pod size is one of the important agronomic traits of peanut variety, with a direct impact on peanut yield. Methods: In a previous study, AhRUVBL2 was identified by map-based cloning technology as a candidate gene that regulates peanut pod size. Results: Overexpression of AhRUVBL2 in transgenic Arabidopsis significantly increased plant height, branch number, leaf size, silique size, seed size, and thousand-seed weight. Further examination revealed an increase in the area of silique exocarp cells, and the number and area of endocarp lignified cells. A total of 337 differentially expressed genes, including PRX (Periaxin) , SAUR (Small Auxin-up RNA), and PYL (Pyrabactin Resistance 1-like), were identified by transcriptome analysis of transgenic Arabidopsis silique. proAhRUVBL2-GUS was found to be expressed explicitly in seeds, and the expression activity of proAhRUVBL2-D893 was significantly greater than that of proAhRUVBL2-79266. Exogenous ABA (abscisic acid) and IAA (indole acetic acid) treatment of proAhRUVBL2-GUS-transformed tobacco leaves revealed that the AhRUVBL2 promoter was hormone-responsive. Conclusions: This study sheds light on the function of AhRUVBL2 in regulating plant growth and development. Moreover, characterization of the AhRUVBL2 promoter provides a valuable genetic resource for enhancing peanut yield.
ISSN:	2768-6701