Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation.
We examined the magnetic effect of thaumatin crystals, which are a well known model of protein crystals but which have hardly been studied for that effect. We succeeded in crystallizing thaumatin by magnetic levitation based on the magneto-Archimedes effect by the addition of the paramagnetic substa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0315335 |
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| author | Syou Maki Masao Fujiwara Yoshihisa Fujiwara Makoto Nakabayashi Shotaro Morimoto Seiichi Tanaka Seiji Fujiwara Masayuki Hagiwara Kohji Inaka |
| author_facet | Syou Maki Masao Fujiwara Yoshihisa Fujiwara Makoto Nakabayashi Shotaro Morimoto Seiichi Tanaka Seiji Fujiwara Masayuki Hagiwara Kohji Inaka |
| author_sort | Syou Maki |
| collection | DOAJ |
| description | We examined the magnetic effect of thaumatin crystals, which are a well known model of protein crystals but which have hardly been studied for that effect. We succeeded in crystallizing thaumatin by magnetic levitation based on the magneto-Archimedes effect by the addition of the paramagnetic substance gadolinium chloride. We also carried out a chronological observation of the levitation process in a superconducting magnet, and visualized the magnetic orientation of the crystals by applying a magnetic field along the horizontal direction. In another major result, we carried out a diffraction experiment and performed a structural analysis of the crystals. We noticed from the results that no electron density from the gadolinium ion could be observed in the crystals. This suggests the possibility that the paramagnetic substance of the aqueous precipitant solution helps only to promote the crystals' levitation, and has little effect on thaumatin crystallization. |
| format | Article |
| id | doaj-art-4b9b2d7f5a59490a89b3ef890f2514a8 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-4b9b2d7f5a59490a89b3ef890f2514a82025-08-20T02:56:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031533510.1371/journal.pone.0315335Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation.Syou MakiMasao FujiwaraYoshihisa FujiwaraMakoto NakabayashiShotaro MorimotoSeiichi TanakaSeiji FujiwaraMasayuki HagiwaraKohji InakaWe examined the magnetic effect of thaumatin crystals, which are a well known model of protein crystals but which have hardly been studied for that effect. We succeeded in crystallizing thaumatin by magnetic levitation based on the magneto-Archimedes effect by the addition of the paramagnetic substance gadolinium chloride. We also carried out a chronological observation of the levitation process in a superconducting magnet, and visualized the magnetic orientation of the crystals by applying a magnetic field along the horizontal direction. In another major result, we carried out a diffraction experiment and performed a structural analysis of the crystals. We noticed from the results that no electron density from the gadolinium ion could be observed in the crystals. This suggests the possibility that the paramagnetic substance of the aqueous precipitant solution helps only to promote the crystals' levitation, and has little effect on thaumatin crystallization.https://doi.org/10.1371/journal.pone.0315335 |
| spellingShingle | Syou Maki Masao Fujiwara Yoshihisa Fujiwara Makoto Nakabayashi Shotaro Morimoto Seiichi Tanaka Seiji Fujiwara Masayuki Hagiwara Kohji Inaka Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation. PLoS ONE |
| title | Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation. |
| title_full | Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation. |
| title_fullStr | Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation. |
| title_full_unstemmed | Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation. |
| title_short | Magnetic effects of thaumatin crystals; observation of crystal growth by magneto-Archimedes levitation and magnetic orientation. |
| title_sort | magnetic effects of thaumatin crystals observation of crystal growth by magneto archimedes levitation and magnetic orientation |
| url | https://doi.org/10.1371/journal.pone.0315335 |
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