The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity
The aim of our study was to investigate the effect of quercetin on cyclophosphamideinduced cardiotoxicity. A total of 35 female rats were used in the study, divided into five groups of seven rats each. All of the rats, except those in the control group, underwent ovariectomy and had their ovaries re...
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Kafkas University, Faculty of Veterinary Medicine
2025-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3193 |
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| author | Şükran YEDİEL ARAS Mustafa MAKAV Mushap KURU Metin ÖĞÜN Ebru KARADAĞ SARI Arzu GEZER Sevda ELİŞ YILDIZ |
| author_facet | Şükran YEDİEL ARAS Mustafa MAKAV Mushap KURU Metin ÖĞÜN Ebru KARADAĞ SARI Arzu GEZER Sevda ELİŞ YILDIZ |
| author_sort | Şükran YEDİEL ARAS |
| collection | DOAJ |
| description | The aim of our study was to investigate the effect of quercetin on cyclophosphamideinduced
cardiotoxicity. A total of 35 female rats were used in the study, divided into five
groups of seven rats each. All of the rats, except those in the control group, underwent
ovariectomy and had their ovaries removed. This eliminated the potential impact
of hormones such as oestrogen and progesterone on the study. All rats were fed ad
libitum. While the control and ovariectomy groups received no treatment, 50 mg/kg
quercetin (oral) was administered to the quercetin group, 100 mg/kg cyclophosphamide
(intraperitoneal) to the cyclophosphamide group, and 50 mg/kg quercetin (oral) and
100 mg/kg cyclophosphamide to the quercetin + cyclophosphamide group was applied.
electrocardiography (ECG) measurements were taken before toxicity induction (day 0)
and on day 5 after toxicity induction. At the end of the study, the rats were euthanized
under anesthesia in accordance with ethical rules, and tissue and blood samples necessary
for analysis were taken. Troponin, creatine kinase (CK), and creatinine kinase MB
(CK-MB) parameters were measured in the blood samples taken. Histopathological
and immunohistochemical analyses (desmin and vimentin) were performed on heart
tissue. According to the analysis, an increase in troponin, CK, and CK-MB parameters
was observed in the cyclophosphamide group, while a decrease was observed in the
quercetin group. In desmin and vimentin immunoreactivity, a decrease was observed
in the cyclophosphamide group, while an increase was observed in the quercetin group.
In conclusion, in our study, we demonstrated that quercetin has a positive effect against
cyclophosphamide-induced cardiotoxicity. |
| format | Article |
| id | doaj-art-4b928e40aed443a2afb7d4df7f499776 |
| institution | DOAJ |
| issn | 1309-2251 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-4b928e40aed443a2afb7d4df7f4997762025-08-20T03:23:51ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512025-05-0131337738510.9775/kvfd.2025.336353193The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicityŞükran YEDİEL ARAS0Mustafa MAKAV1Mushap KURU2Metin ÖĞÜN3Ebru KARADAĞ SARI4Arzu GEZER5Sevda ELİŞ YILDIZ6Kafkas University, Faculty of Health Sciences, Department of Midwifery, TR-36100 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Physiology, TR-36100 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-36100 Kars - TÜRKİYEKafkas University, Faculty of Medicine, Department of Biochemistry, TR-36100 Kars - TÜRKİYEKafkas University, Faculty of Veterinary Medicine, Department of Histology-Embryology, TR-36100 Kars - TÜRKİYEAtatürk University, Vocational School of Health Services, TR-25100 Erzurum - TÜRKİYEKafkas University, Faculty of Health Sciences, Department of Midwifery, TR-36100 Kars - TÜRKİYEThe aim of our study was to investigate the effect of quercetin on cyclophosphamideinduced cardiotoxicity. A total of 35 female rats were used in the study, divided into five groups of seven rats each. All of the rats, except those in the control group, underwent ovariectomy and had their ovaries removed. This eliminated the potential impact of hormones such as oestrogen and progesterone on the study. All rats were fed ad libitum. While the control and ovariectomy groups received no treatment, 50 mg/kg quercetin (oral) was administered to the quercetin group, 100 mg/kg cyclophosphamide (intraperitoneal) to the cyclophosphamide group, and 50 mg/kg quercetin (oral) and 100 mg/kg cyclophosphamide to the quercetin + cyclophosphamide group was applied. electrocardiography (ECG) measurements were taken before toxicity induction (day 0) and on day 5 after toxicity induction. At the end of the study, the rats were euthanized under anesthesia in accordance with ethical rules, and tissue and blood samples necessary for analysis were taken. Troponin, creatine kinase (CK), and creatinine kinase MB (CK-MB) parameters were measured in the blood samples taken. Histopathological and immunohistochemical analyses (desmin and vimentin) were performed on heart tissue. According to the analysis, an increase in troponin, CK, and CK-MB parameters was observed in the cyclophosphamide group, while a decrease was observed in the quercetin group. In desmin and vimentin immunoreactivity, a decrease was observed in the cyclophosphamide group, while an increase was observed in the quercetin group. In conclusion, in our study, we demonstrated that quercetin has a positive effect against cyclophosphamide-induced cardiotoxicity.https://vetdergikafkas.org/pdf.php?id=3193cyclophosphamidetroponincreatine kinaseck-mbdesminvimentin |
| spellingShingle | Şükran YEDİEL ARAS Mustafa MAKAV Mushap KURU Metin ÖĞÜN Ebru KARADAĞ SARI Arzu GEZER Sevda ELİŞ YILDIZ The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity Kafkas Universitesi Veteriner Fakültesi Dergisi cyclophosphamide troponin creatine kinase ck-mb desmin vimentin |
| title | The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity |
| title_full | The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity |
| title_fullStr | The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity |
| title_full_unstemmed | The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity |
| title_short | The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-induced cardiotoxicity |
| title_sort | effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide induced cardiotoxicity |
| topic | cyclophosphamide troponin creatine kinase ck-mb desmin vimentin |
| url | https://vetdergikafkas.org/pdf.php?id=3193 |
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