A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging

Abstract The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR spectral activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of dye aggregation remains ch...

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Main Authors: Cheng Yao, Ruwei Wei, Xiao Luo, Jie Zhou, Xiaodong Zhang, Xicun Lu, Yan Dong, Ruofan Chu, Yuxin Sun, Yu Wang, Wencheng Xia, Dahui Qu, Cong Liu, Jun Ren, Guangbo Ge, Jinquan Chen, Xuhong Qian, Youjun Yang
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-55445-x
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author Cheng Yao
Ruwei Wei
Xiao Luo
Jie Zhou
Xiaodong Zhang
Xicun Lu
Yan Dong
Ruofan Chu
Yuxin Sun
Yu Wang
Wencheng Xia
Dahui Qu
Cong Liu
Jun Ren
Guangbo Ge
Jinquan Chen
Xuhong Qian
Youjun Yang
author_facet Cheng Yao
Ruwei Wei
Xiao Luo
Jie Zhou
Xiaodong Zhang
Xicun Lu
Yan Dong
Ruofan Chu
Yuxin Sun
Yu Wang
Wencheng Xia
Dahui Qu
Cong Liu
Jun Ren
Guangbo Ge
Jinquan Chen
Xuhong Qian
Youjun Yang
author_sort Cheng Yao
collection DOAJ
description Abstract The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR spectral activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of dye aggregation remains challenging. We propose a crystal-aided aggregate synthesis (CAASH) approach to introduce a layer of rationality for the development of J-aggregate and the successful development of a water-soluble SWIR JV-aggregate with a bisbenzannulated silicon rhodamine scaffold (ESi5). The resulting SWIR-aggregates exhibit excellent stabilities toward organic solvents, pH, sonication, photobleaching, thiols, and endogenous oxidative species. Notably, the aggregates have a high structure-dependent melting temperature of ca. 330-335 K. In fact, the heating/annealing process can be exploited to reduce aggregation disorder. The aggregates are biocompatible and have broad potential in in vivo fluorescence and photoacoustic imaging and more.
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issn 2041-1723
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publishDate 2025-01-01
publisher Nature Portfolio
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series Nature Communications
spelling doaj-art-4b917c4d3ff7466e8123323f741b31442025-08-20T02:46:00ZengNature PortfolioNature Communications2041-17232025-01-0116111210.1038/s41467-024-55445-xA stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imagingCheng Yao0Ruwei Wei1Xiao Luo2Jie Zhou3Xiaodong Zhang4Xicun Lu5Yan Dong6Ruofan Chu7Yuxin Sun8Yu Wang9Wencheng Xia10Dahui Qu11Cong Liu12Jun Ren13Guangbo Ge14Jinquan Chen15Xuhong Qian16Youjun Yang17State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyShanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal UniversityState Key Laboratory of Precision Spectroscopy, East China Normal UniversityState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyShanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal UniversityInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyInterdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of SciencesHubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals & Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei UniversityInstitute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese MedicineState Key Laboratory of Precision Spectroscopy, East China Normal UniversityState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyAbstract The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR spectral activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of dye aggregation remains challenging. We propose a crystal-aided aggregate synthesis (CAASH) approach to introduce a layer of rationality for the development of J-aggregate and the successful development of a water-soluble SWIR JV-aggregate with a bisbenzannulated silicon rhodamine scaffold (ESi5). The resulting SWIR-aggregates exhibit excellent stabilities toward organic solvents, pH, sonication, photobleaching, thiols, and endogenous oxidative species. Notably, the aggregates have a high structure-dependent melting temperature of ca. 330-335 K. In fact, the heating/annealing process can be exploited to reduce aggregation disorder. The aggregates are biocompatible and have broad potential in in vivo fluorescence and photoacoustic imaging and more.https://doi.org/10.1038/s41467-024-55445-x
spellingShingle Cheng Yao
Ruwei Wei
Xiao Luo
Jie Zhou
Xiaodong Zhang
Xicun Lu
Yan Dong
Ruofan Chu
Yuxin Sun
Yu Wang
Wencheng Xia
Dahui Qu
Cong Liu
Jun Ren
Guangbo Ge
Jinquan Chen
Xuhong Qian
Youjun Yang
A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging
Nature Communications
title A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging
title_full A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging
title_fullStr A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging
title_full_unstemmed A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging
title_short A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging
title_sort stable and biocompatible shortwave infrared nanoribbon for dual channel in vivo imaging
url https://doi.org/10.1038/s41467-024-55445-x
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