Transcriptomic insights into soybean seed deterioration in uncontrolled storage
ABSTRACT Soybean, the main agricultural commodity of Brazil, is sensitive to deterioration during storage due to its high fatty acid content and fragile seed coat, especially under tropical conditions where high temperature and humidity accelerate this process. The objective of this study is to inve...
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| Language: | English |
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Associação Brasileira de Tecnologia de Sementes
2025-03-01
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| Series: | Journal of Seed Science |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372025000100201&lng=en&tlng=en |
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| author | João Matheus Kafer Mayla Daiane Correa Molinari Fernando Augusto Henning Lucas Vinicius de Sousa Alcantara Alexandre Lima Nepomuceno Liliane Marcia Mertz-Henning |
| author_facet | João Matheus Kafer Mayla Daiane Correa Molinari Fernando Augusto Henning Lucas Vinicius de Sousa Alcantara Alexandre Lima Nepomuceno Liliane Marcia Mertz-Henning |
| author_sort | João Matheus Kafer |
| collection | DOAJ |
| description | ABSTRACT Soybean, the main agricultural commodity of Brazil, is sensitive to deterioration during storage due to its high fatty acid content and fragile seed coat, especially under tropical conditions where high temperature and humidity accelerate this process. The objective of this study is to investigate the mechanisms that are altered during the storage of soybean seeds under uncontrolled environmental conditions. For this purpose, RNA-seq libraries were generated from freshly harvested soybean seeds (FHS) and seeds stored under uncontrolled conditions (UES). The total RNA from seeds of the cultivar BRS 413 was extracted and sequenced for gene expression analysis. Relative expression indicated 325 genes repressed in UES and 150 induced when comparing the genes in stored seeds to freshly harvested ones. Genes related to protein folding, carbohydrate metabolism, lipids, phytohormones, and transcription factors showed a significant reduction in their expression during the storage. Among the genes uniquely expressed in UES, alanine-glyoxylate transaminase (GGAT), cysteine synthase (CSase), aldehyde dehydrogenase (ALDH), thioredoxin peroxidase (Peroxiredoxin), cytochrome P450 and protein phosphatase 2C (PP2C) stood out for making part of protective mechanisms against oxidative stress in plants. |
| format | Article |
| id | doaj-art-4b8ac6c5d1fd4a47b5b0a204008b2f4e |
| institution | OA Journals |
| issn | 2317-1545 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Associação Brasileira de Tecnologia de Sementes |
| record_format | Article |
| series | Journal of Seed Science |
| spelling | doaj-art-4b8ac6c5d1fd4a47b5b0a204008b2f4e2025-08-20T02:08:40ZengAssociação Brasileira de Tecnologia de SementesJournal of Seed Science2317-15452025-03-014710.1590/2317-1545v47289243Transcriptomic insights into soybean seed deterioration in uncontrolled storageJoão Matheus Kaferhttps://orcid.org/0000-0003-2339-8683Mayla Daiane Correa Molinarihttps://orcid.org/0000-0002-9135-0422Fernando Augusto Henninghttps://orcid.org/0000-0002-1705-9047Lucas Vinicius de Sousa Alcantarahttps://orcid.org/0000-0003-0233-914XAlexandre Lima Nepomucenohttps://orcid.org/0000-0003-2012-6201Liliane Marcia Mertz-Henninghttps://orcid.org/0000-0001-7622-0649ABSTRACT Soybean, the main agricultural commodity of Brazil, is sensitive to deterioration during storage due to its high fatty acid content and fragile seed coat, especially under tropical conditions where high temperature and humidity accelerate this process. The objective of this study is to investigate the mechanisms that are altered during the storage of soybean seeds under uncontrolled environmental conditions. For this purpose, RNA-seq libraries were generated from freshly harvested soybean seeds (FHS) and seeds stored under uncontrolled conditions (UES). The total RNA from seeds of the cultivar BRS 413 was extracted and sequenced for gene expression analysis. Relative expression indicated 325 genes repressed in UES and 150 induced when comparing the genes in stored seeds to freshly harvested ones. Genes related to protein folding, carbohydrate metabolism, lipids, phytohormones, and transcription factors showed a significant reduction in their expression during the storage. Among the genes uniquely expressed in UES, alanine-glyoxylate transaminase (GGAT), cysteine synthase (CSase), aldehyde dehydrogenase (ALDH), thioredoxin peroxidase (Peroxiredoxin), cytochrome P450 and protein phosphatase 2C (PP2C) stood out for making part of protective mechanisms against oxidative stress in plants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372025000100201&lng=en&tlng=enGlycine max L.RNA-seqseed viability |
| spellingShingle | João Matheus Kafer Mayla Daiane Correa Molinari Fernando Augusto Henning Lucas Vinicius de Sousa Alcantara Alexandre Lima Nepomuceno Liliane Marcia Mertz-Henning Transcriptomic insights into soybean seed deterioration in uncontrolled storage Journal of Seed Science Glycine max L. RNA-seq seed viability |
| title | Transcriptomic insights into soybean seed deterioration in uncontrolled storage |
| title_full | Transcriptomic insights into soybean seed deterioration in uncontrolled storage |
| title_fullStr | Transcriptomic insights into soybean seed deterioration in uncontrolled storage |
| title_full_unstemmed | Transcriptomic insights into soybean seed deterioration in uncontrolled storage |
| title_short | Transcriptomic insights into soybean seed deterioration in uncontrolled storage |
| title_sort | transcriptomic insights into soybean seed deterioration in uncontrolled storage |
| topic | Glycine max L. RNA-seq seed viability |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372025000100201&lng=en&tlng=en |
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