TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population
Background: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2007/418247 |
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| author | Indu Kohaar Nisha Thakur Sudha Salhan Swaraj Batra Veena Singh Anita Sharma Pushpa Sodhani B. C. Das Debi P. Sarkar Mausumi Bharadwaj |
| author_facet | Indu Kohaar Nisha Thakur Sudha Salhan Swaraj Batra Veena Singh Anita Sharma Pushpa Sodhani B. C. Das Debi P. Sarkar Mausumi Bharadwaj |
| author_sort | Indu Kohaar |
| collection | DOAJ |
| description | Background: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45 precancer and 120 cancer patients and an equal number (165) of healthy controls with normal cervical cytology. PCR-RFLP was employed to analyze TNFα promoter polymorphisms, which were confirmed by direct sequencing. Both patients and controls were screened for Human Papillomavirus (HPV) infection. Results: The frequency of –308 A allele in TNFα was significantly higher in cases compared with control subjects (21% in cases vs. 9% in controls; p < 0.01), with an odds ratio of 2.7 (95% CI = 1.41–5.15). Also, women carrying A allele for this locus presented 3 times increased susceptibility to HPV 16 infection as evident from carrier genotype distribution between HPV positive cases and control subjects (24% in HPV positive cases vs. 9% in controls; p < 0.01; OR = 3.1; 95% CI = 1.60–6.03). No such association was found for TNFα–238 (G/A) polymorphism with the risk of development of cervical cancer. Conclusion: It suggests that SNP at –308 (G/A) of TNFα promoter may represent an increased risk for HPV infection and development of cervical cancer in Indian women. |
| format | Article |
| id | doaj-art-4b87d4d1df544f01afd3d488d3a8a6c6 |
| institution | OA Journals |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-4b87d4d1df544f01afd3d488d3a8a6c62025-08-20T02:38:41ZengWileyCellular Oncology1570-58701875-86062007-01-0129324925610.1155/2007/418247TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian PopulationIndu Kohaar0Nisha Thakur1Sudha Salhan2Swaraj Batra3Veena Singh4Anita Sharma5Pushpa Sodhani6B. C. Das7Debi P. Sarkar8Mausumi Bharadwaj9Division of Molecular Genetics & Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaDivision of Molecular Genetics & Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaDepartment of Obstetrics and Gynaecology, Safdarjung Hospital, New Delhi, IndiaDepartment of Obstetrics & Gynaecology, LNJP Hospital, New Delhi, IndiaDivision of Clinical Oncology, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaDivision of Molecular Genetics & Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaDivision of Cytopathology, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaDivision of Molecular Oncology, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaDepartment of Biochemistry, University of Delhi (South Campus), New Delhi, IndiaDivision of Molecular Genetics & Biochemistry, Institute of Cytology & Preventive Oncology (ICMR), I-7, Sector 39, Noida, IndiaBackground: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at –308 G/A and –238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45 precancer and 120 cancer patients and an equal number (165) of healthy controls with normal cervical cytology. PCR-RFLP was employed to analyze TNFα promoter polymorphisms, which were confirmed by direct sequencing. Both patients and controls were screened for Human Papillomavirus (HPV) infection. Results: The frequency of –308 A allele in TNFα was significantly higher in cases compared with control subjects (21% in cases vs. 9% in controls; p < 0.01), with an odds ratio of 2.7 (95% CI = 1.41–5.15). Also, women carrying A allele for this locus presented 3 times increased susceptibility to HPV 16 infection as evident from carrier genotype distribution between HPV positive cases and control subjects (24% in HPV positive cases vs. 9% in controls; p < 0.01; OR = 3.1; 95% CI = 1.60–6.03). No such association was found for TNFα–238 (G/A) polymorphism with the risk of development of cervical cancer. Conclusion: It suggests that SNP at –308 (G/A) of TNFα promoter may represent an increased risk for HPV infection and development of cervical cancer in Indian women.http://dx.doi.org/10.1155/2007/418247 |
| spellingShingle | Indu Kohaar Nisha Thakur Sudha Salhan Swaraj Batra Veena Singh Anita Sharma Pushpa Sodhani B. C. Das Debi P. Sarkar Mausumi Bharadwaj TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population Cellular Oncology |
| title | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
| title_full | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
| title_fullStr | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
| title_full_unstemmed | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
| title_short | TNFα–308G/A Polymorphism as a Risk Factor for HPV Associated Cervical Cancer in Indian Population |
| title_sort | tnfα 308g a polymorphism as a risk factor for hpv associated cervical cancer in indian population |
| url | http://dx.doi.org/10.1155/2007/418247 |
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