Conserved pattern-based classification of human odorant receptor multigene family
Abstract Conserved protein-coding sequences are critical for maintaining protein function across species. Odorant receptors (ORs), a large poorly understood multigene family responsible for odor detection, lack comprehensive classification methods that reflect their functional diversity. In this stu...
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| Format: | Article |
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-79183-8 |
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| author | Sang Eun Ryu Jisub Bae Tammy Shim Won-Cheol Kim Kwangsu Kim Cheil Moon |
| author_facet | Sang Eun Ryu Jisub Bae Tammy Shim Won-Cheol Kim Kwangsu Kim Cheil Moon |
| author_sort | Sang Eun Ryu |
| collection | DOAJ |
| description | Abstract Conserved protein-coding sequences are critical for maintaining protein function across species. Odorant receptors (ORs), a large poorly understood multigene family responsible for odor detection, lack comprehensive classification methods that reflect their functional diversity. In this study, we propose a new approach called conserved motif-based classification (CMC) for classifying ORs based on amino acid sequence similarities within conserved motifs. Specifically, we focused on three well-conserved motifs: MAYDRYVAIC in TM3, KAFSTCASH in TM6, and PMLNPFIY in TM7. Using an unsupervised clustering technique, we classified human ORs (hORs) into two main clusters with six sub-clusters. CMC partly reflects previously identified subfamilies, revealing altered residue positions among the sub-clusters. These altered positions interacted with specific residues within or adjacent to the transmembrane domain, suggesting functional implications. Furthermore, we found that the CMC correlated with both ligand responses and ectopic expression patterns, highlighting its relevance to OR function. This conserved motif-based classification will help in understanding the functions and features that are not understood by classification based solely on entire amino acid sequence similarity. |
| format | Article |
| id | doaj-art-4b84769f5398451bae37d6485fd6928c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-4b84769f5398451bae37d6485fd6928c2025-01-26T12:34:57ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-79183-8Conserved pattern-based classification of human odorant receptor multigene familySang Eun Ryu0Jisub Bae1Tammy Shim2Won-Cheol Kim3Kwangsu Kim4Cheil Moon5Department of Brain Sciences, Graduate School, Daegu Gyeongbuk Institute of Science and Technology (DGIST)Department of Brain Sciences, Graduate School, Daegu Gyeongbuk Institute of Science and Technology (DGIST)Department of Brain Sciences, Graduate School, Daegu Gyeongbuk Institute of Science and Technology (DGIST)Department of Brain Sciences, Graduate School, Daegu Gyeongbuk Institute of Science and Technology (DGIST)Department of Brain Sciences, Graduate School, Daegu Gyeongbuk Institute of Science and Technology (DGIST)Department of Brain Sciences, Graduate School, Daegu Gyeongbuk Institute of Science and Technology (DGIST)Abstract Conserved protein-coding sequences are critical for maintaining protein function across species. Odorant receptors (ORs), a large poorly understood multigene family responsible for odor detection, lack comprehensive classification methods that reflect their functional diversity. In this study, we propose a new approach called conserved motif-based classification (CMC) for classifying ORs based on amino acid sequence similarities within conserved motifs. Specifically, we focused on three well-conserved motifs: MAYDRYVAIC in TM3, KAFSTCASH in TM6, and PMLNPFIY in TM7. Using an unsupervised clustering technique, we classified human ORs (hORs) into two main clusters with six sub-clusters. CMC partly reflects previously identified subfamilies, revealing altered residue positions among the sub-clusters. These altered positions interacted with specific residues within or adjacent to the transmembrane domain, suggesting functional implications. Furthermore, we found that the CMC correlated with both ligand responses and ectopic expression patterns, highlighting its relevance to OR function. This conserved motif-based classification will help in understanding the functions and features that are not understood by classification based solely on entire amino acid sequence similarity.https://doi.org/10.1038/s41598-024-79183-8Odorant receptorConserved motifClassificationOlfactoryGPCR |
| spellingShingle | Sang Eun Ryu Jisub Bae Tammy Shim Won-Cheol Kim Kwangsu Kim Cheil Moon Conserved pattern-based classification of human odorant receptor multigene family Scientific Reports Odorant receptor Conserved motif Classification Olfactory GPCR |
| title | Conserved pattern-based classification of human odorant receptor multigene family |
| title_full | Conserved pattern-based classification of human odorant receptor multigene family |
| title_fullStr | Conserved pattern-based classification of human odorant receptor multigene family |
| title_full_unstemmed | Conserved pattern-based classification of human odorant receptor multigene family |
| title_short | Conserved pattern-based classification of human odorant receptor multigene family |
| title_sort | conserved pattern based classification of human odorant receptor multigene family |
| topic | Odorant receptor Conserved motif Classification Olfactory GPCR |
| url | https://doi.org/10.1038/s41598-024-79183-8 |
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