Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier
A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Ts...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences Committee of Chemical and Process Engineering
2016-03-01
|
| Series: | Chemical and Process Engineering |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/cpe.2016.37.issue-1/cpe-2016-0014/cpe-2016-0014.xml?format=INT |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850107012230152192 |
|---|---|
| author | Pilarek Maciej Dąbkowska Katarzyna |
| author_facet | Pilarek Maciej Dąbkowska Katarzyna |
| author_sort | Pilarek Maciej |
| collection | DOAJ |
| description | A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass. |
| format | Article |
| id | doaj-art-4b81f0f4fde84a2cb2c471f62ab51233 |
| institution | OA Journals |
| issn | 2300-1925 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Polish Academy of Sciences Committee of Chemical and Process Engineering |
| record_format | Article |
| series | Chemical and Process Engineering |
| spelling | doaj-art-4b81f0f4fde84a2cb2c471f62ab512332025-08-20T02:38:41ZengPolish Academy of Sciences Committee of Chemical and Process EngineeringChemical and Process Engineering2300-19252016-03-0137114915810.1515/cpe-2016-0014cpe-2016-0014Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrierPilarek Maciej0Dąbkowska Katarzyna1Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, PolandWarsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, PolandA mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass.http://www.degruyter.com/view/j/cpe.2016.37.issue-1/cpe-2016-0014/cpe-2016-0014.xml?format=INTliquid perfluorochemical (fluorocarbon)liquid gas carriermathematical modellingcell/tissue culturehybrid culture system |
| spellingShingle | Pilarek Maciej Dąbkowska Katarzyna Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier Chemical and Process Engineering liquid perfluorochemical (fluorocarbon) liquid gas carrier mathematical modelling cell/tissue culture hybrid culture system |
| title | Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier |
| title_full | Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier |
| title_fullStr | Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier |
| title_full_unstemmed | Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier |
| title_short | Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier |
| title_sort | modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier |
| topic | liquid perfluorochemical (fluorocarbon) liquid gas carrier mathematical modelling cell/tissue culture hybrid culture system |
| url | http://www.degruyter.com/view/j/cpe.2016.37.issue-1/cpe-2016-0014/cpe-2016-0014.xml?format=INT |
| work_keys_str_mv | AT pilarekmaciej modellingofahybridculturesystemwithastationarylayerofliquidperfluorochemicalappliedasoxygencarrier AT dabkowskakatarzyna modellingofahybridculturesystemwithastationarylayerofliquidperfluorochemicalappliedasoxygencarrier |