Development of a novel fluorescent gauze using indocyanine green
Abstract Gauzes are used in various ways, including wiping blood, compressing organs, and as markers during surgery. However, losing gauze during surgery can lead to pieces being left inside the body, causing time-consuming searches and unnecessary radiation exposure. To address this, we developed a...
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Nature Portfolio
2025-03-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-94944-9 |
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| author | Takeshi Urade Shinobu Tsuchida Kentaro Oji Yoshiaki Fujiwara Yasushi Fukuoka Takahiro Yasuda Takumi Fukumoto |
| author_facet | Takeshi Urade Shinobu Tsuchida Kentaro Oji Yoshiaki Fujiwara Yasushi Fukuoka Takahiro Yasuda Takumi Fukumoto |
| author_sort | Takeshi Urade |
| collection | DOAJ |
| description | Abstract Gauzes are used in various ways, including wiping blood, compressing organs, and as markers during surgery. However, losing gauze during surgery can lead to pieces being left inside the body, causing time-consuming searches and unnecessary radiation exposure. To address this, we developed a fluorescent gauze using indocyanine green (ICG), which fluoresces under near-infrared (NIR) light. This study aimed to evaluate the fluorescence intensity (FI) of the gauze and confirm its fluorescence in a porcine abdominal cavity. We dissolved 25 mg of ICG in 10 ml of glycerol, ethanol, distilled water, and 5% bovine serum albumin, then diluted each solution 5- to 106-fold with distilled water. The gauze was dyed using these solutions. A rigid laparoscope observed the fluorescent gauze in a dark room under NIR light, and FI was measured. The gauze treated with a 100-fold dilution of each ICG dyestuff showed the strongest fluorescence. This gauze was then placed in a pig’s abdominal cavity and observed under NIR light, demonstrating that the fluorescence could penetrate two or three layers of the mesentery. Our fluorescent gauze, confirmed both ex vivo and in vivo, should be manufactured for clinical use and further validated for its utility. |
| format | Article |
| id | doaj-art-4b7c3798dceb40a98e4a9a8a733f7b41 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4b7c3798dceb40a98e4a9a8a733f7b412025-08-20T02:10:12ZengNature PortfolioScientific Reports2045-23222025-03-011511710.1038/s41598-025-94944-9Development of a novel fluorescent gauze using indocyanine greenTakeshi Urade0Shinobu Tsuchida1Kentaro Oji2Yoshiaki Fujiwara3Yasushi Fukuoka4Takahiro Yasuda5Takumi Fukumoto6Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of MedicineDepartment of Surgery, Mitsubishi Kobe HospitalDepartment of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of MedicineCenter for Advanced Medical Engineering Research and Development, Kobe University HospitalCenter for Advanced Medical Engineering Research and Development, Kobe University HospitalCenter for Advanced Medical Engineering Research and Development, Kobe University HospitalDepartment of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of MedicineAbstract Gauzes are used in various ways, including wiping blood, compressing organs, and as markers during surgery. However, losing gauze during surgery can lead to pieces being left inside the body, causing time-consuming searches and unnecessary radiation exposure. To address this, we developed a fluorescent gauze using indocyanine green (ICG), which fluoresces under near-infrared (NIR) light. This study aimed to evaluate the fluorescence intensity (FI) of the gauze and confirm its fluorescence in a porcine abdominal cavity. We dissolved 25 mg of ICG in 10 ml of glycerol, ethanol, distilled water, and 5% bovine serum albumin, then diluted each solution 5- to 106-fold with distilled water. The gauze was dyed using these solutions. A rigid laparoscope observed the fluorescent gauze in a dark room under NIR light, and FI was measured. The gauze treated with a 100-fold dilution of each ICG dyestuff showed the strongest fluorescence. This gauze was then placed in a pig’s abdominal cavity and observed under NIR light, demonstrating that the fluorescence could penetrate two or three layers of the mesentery. Our fluorescent gauze, confirmed both ex vivo and in vivo, should be manufactured for clinical use and further validated for its utility.https://doi.org/10.1038/s41598-025-94944-9Fluorescent gauzeGauzeIndocyanine greenNear-infrared fluorescenceNear-infrared imaging |
| spellingShingle | Takeshi Urade Shinobu Tsuchida Kentaro Oji Yoshiaki Fujiwara Yasushi Fukuoka Takahiro Yasuda Takumi Fukumoto Development of a novel fluorescent gauze using indocyanine green Scientific Reports Fluorescent gauze Gauze Indocyanine green Near-infrared fluorescence Near-infrared imaging |
| title | Development of a novel fluorescent gauze using indocyanine green |
| title_full | Development of a novel fluorescent gauze using indocyanine green |
| title_fullStr | Development of a novel fluorescent gauze using indocyanine green |
| title_full_unstemmed | Development of a novel fluorescent gauze using indocyanine green |
| title_short | Development of a novel fluorescent gauze using indocyanine green |
| title_sort | development of a novel fluorescent gauze using indocyanine green |
| topic | Fluorescent gauze Gauze Indocyanine green Near-infrared fluorescence Near-infrared imaging |
| url | https://doi.org/10.1038/s41598-025-94944-9 |
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