Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products
Alkaline protease being active in neutral to alkaline pH has huge demands in food, detergent, leather and pharmaceutical industries. Its production from agro-industrial wastes not only lowers the production costs but also reduces the environmental problems. Hence, the present study aimed to searc...
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2021-06-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_178300_b62a92c721ab3be138eebb73f19166c8.pdf |
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| author | Bikash Chandra Behera Bijay Kumar Sethi Sonali Mohapatra Hrudayanath Thatoi Rashmi Ranjan Mishra |
| author_facet | Bikash Chandra Behera Bijay Kumar Sethi Sonali Mohapatra Hrudayanath Thatoi Rashmi Ranjan Mishra |
| author_sort | Bikash Chandra Behera |
| collection | DOAJ |
| description | Alkaline protease being active in neutral to alkaline pH has huge demands in food,
detergent, leather and pharmaceutical industries. Its production from agro-industrial wastes not
only lowers the production costs but also reduces the environmental problems. Hence, the
present study aimed to search for new potential microbes, which can produce alkaline protease
enzyme, to meet the industrial demands. In this study, 13 fungal spp. were isolated on potato
dextrose agar medium (PDA) from mangrove soil through serial dilution, and then were
streaked on the skim milk agar medium for qualitative screening of protease production. Out
of 13 fungal spp.; only 7 spp. were able to produce proteolytic zones through the proteolytic
assay. The Relative enzymatic index (REI) value (Zone diameter/Colony diameter) of all the
fungal isolates that produced proteolytic zones on skim milk agar medium was evaluated.
Only 2 fungal isolates which showed maximum REI value were selected, and then identified
morphologically and molecularly as Trichoderma longibrachiatum (Accession no. MF144551)
and by Penicillium rubidurum (Accession no. MF144561). Submerged fermentation was
carried out using different agro industrial substrates to quantify for protease production, where
the supernatants obtained were used for alkaline protease estimation. Among the different
tested substrates, soybean powder and wheat bran were the most suitable substrates for
maximum protease production by T. longibrachiatum (233.78±7.12 U/ mg) and P. rubidurum
(228.61±11.13 U/ mg), respectively. The partial purified enzyme from these fungi showed
maximum proteolytic potentials at pH 8.0 (P. rubidurum) and pH 9.0 (T. longibrachiatum),
with optima temperature of 40 °C. Among the tested heavy metals, only Mn2+ expressed
marginal enhancement of the protease enzyme activity. |
| format | Article |
| id | doaj-art-4b5ee6eac0644341adf86c282046f073 |
| institution | OA Journals |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2021-06-01 |
| publisher | ResearchersLinks, Ltd |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-4b5ee6eac0644341adf86c282046f0732025-08-20T02:17:48ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942021-06-01531241125510.21608/nrmj.2021.178300Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial productsBikash Chandra Behera0Bijay Kumar Sethi1Sonali Mohapatra2Hrudayanath Thatoi3Rashmi Ranjan Mishra4Department of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha-751024, IndiaDepartment of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha-751024, IndiaDepartment of Biotechnology, College of Engineering and Technology, Biju Pattnaik University of Technology, Bhubaneswar-751003, IndiaDepartment of Biotechnology, Maharaja Sriram Chandra Bhanja Deo University, Takatpur, Baripada-757003, Odisha, IndiaDepartment of Biotechnology, MITS School of Biotechnology, Bhubaneswar, Odisha-751024, IndiaAlkaline protease being active in neutral to alkaline pH has huge demands in food, detergent, leather and pharmaceutical industries. Its production from agro-industrial wastes not only lowers the production costs but also reduces the environmental problems. Hence, the present study aimed to search for new potential microbes, which can produce alkaline protease enzyme, to meet the industrial demands. In this study, 13 fungal spp. were isolated on potato dextrose agar medium (PDA) from mangrove soil through serial dilution, and then were streaked on the skim milk agar medium for qualitative screening of protease production. Out of 13 fungal spp.; only 7 spp. were able to produce proteolytic zones through the proteolytic assay. The Relative enzymatic index (REI) value (Zone diameter/Colony diameter) of all the fungal isolates that produced proteolytic zones on skim milk agar medium was evaluated. Only 2 fungal isolates which showed maximum REI value were selected, and then identified morphologically and molecularly as Trichoderma longibrachiatum (Accession no. MF144551) and by Penicillium rubidurum (Accession no. MF144561). Submerged fermentation was carried out using different agro industrial substrates to quantify for protease production, where the supernatants obtained were used for alkaline protease estimation. Among the different tested substrates, soybean powder and wheat bran were the most suitable substrates for maximum protease production by T. longibrachiatum (233.78±7.12 U/ mg) and P. rubidurum (228.61±11.13 U/ mg), respectively. The partial purified enzyme from these fungi showed maximum proteolytic potentials at pH 8.0 (P. rubidurum) and pH 9.0 (T. longibrachiatum), with optima temperature of 40 °C. Among the tested heavy metals, only Mn2+ expressed marginal enhancement of the protease enzyme activity.https://nrmj.journals.ekb.eg/article_178300_b62a92c721ab3be138eebb73f19166c8.pdfalkaline proteaseagro-industrial wastefermentationfungi |
| spellingShingle | Bikash Chandra Behera Bijay Kumar Sethi Sonali Mohapatra Hrudayanath Thatoi Rashmi Ranjan Mishra Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products Novel Research in Microbiology Journal alkaline protease agro-industrial waste fermentation fungi |
| title | Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products |
| title_full | Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products |
| title_fullStr | Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products |
| title_full_unstemmed | Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products |
| title_short | Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products |
| title_sort | bio production of alkaline protease by trichoderma longibrachiatum and penicillium rubidurum using different agro industrial products |
| topic | alkaline protease agro-industrial waste fermentation fungi |
| url | https://nrmj.journals.ekb.eg/article_178300_b62a92c721ab3be138eebb73f19166c8.pdf |
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