Bio-production of alkaline protease by Trichoderma longibrachiatum and Penicillium rubidurum using different agro-industrial products
Alkaline protease being active in neutral to alkaline pH has huge demands in food, detergent, leather and pharmaceutical industries. Its production from agro-industrial wastes not only lowers the production costs but also reduces the environmental problems. Hence, the present study aimed to searc...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2021-06-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_178300_b62a92c721ab3be138eebb73f19166c8.pdf |
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| Summary: | Alkaline protease being active in neutral to alkaline pH has huge demands in food,
detergent, leather and pharmaceutical industries. Its production from agro-industrial wastes not
only lowers the production costs but also reduces the environmental problems. Hence, the
present study aimed to search for new potential microbes, which can produce alkaline protease
enzyme, to meet the industrial demands. In this study, 13 fungal spp. were isolated on potato
dextrose agar medium (PDA) from mangrove soil through serial dilution, and then were
streaked on the skim milk agar medium for qualitative screening of protease production. Out
of 13 fungal spp.; only 7 spp. were able to produce proteolytic zones through the proteolytic
assay. The Relative enzymatic index (REI) value (Zone diameter/Colony diameter) of all the
fungal isolates that produced proteolytic zones on skim milk agar medium was evaluated.
Only 2 fungal isolates which showed maximum REI value were selected, and then identified
morphologically and molecularly as Trichoderma longibrachiatum (Accession no. MF144551)
and by Penicillium rubidurum (Accession no. MF144561). Submerged fermentation was
carried out using different agro industrial substrates to quantify for protease production, where
the supernatants obtained were used for alkaline protease estimation. Among the different
tested substrates, soybean powder and wheat bran were the most suitable substrates for
maximum protease production by T. longibrachiatum (233.78±7.12 U/ mg) and P. rubidurum
(228.61±11.13 U/ mg), respectively. The partial purified enzyme from these fungi showed
maximum proteolytic potentials at pH 8.0 (P. rubidurum) and pH 9.0 (T. longibrachiatum),
with optima temperature of 40 °C. Among the tested heavy metals, only Mn2+ expressed
marginal enhancement of the protease enzyme activity. |
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| ISSN: | 2537-0286 2537-0294 |